The rag GTPases bind raptor and mediate amino acid signaling to mTORC1

Science

Volumn 320, Issue 5882, 2008, Pages 1496-1501

  Sancak, Yasemin ^a,b   Peterson, Timothy R ^a,b   Shaul, Yoav D ^a,b   Lindquist, Robert A ^a,b   Thoreen, Carson C ^a,b   Bar Peled, Liron ^a   Sabatini, David M ^a,b,c  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; MAMMALIAN TARGET OF RAPAMYCIN; RAG1 PROTEIN; RHEB PROTEIN; GUANOSINE TRIPHOSPHATE; INSULIN; LEUCINE; MONOMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; MTORC1 PROTEIN, HUMAN; MUTANT PROTEIN; NEUROPEPTIDE; PROTEIN; PROTEIN KINASE; RAPTOR PROTEIN, HUMAN; RHEB PROTEIN, HUMAN; RRAGC PROTEIN, HUMAN; TRANSCRIPTION FACTOR;

AMINO ACID; CANCER; GROWTH RESPONSE; PHOSPHATE; PROTEIN;

ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; CELL LINE; CELL NUCLEUS; CYTOPLASM; DIMERIZATION; GENETICS; METABOLISM; MUTATION; PHOSPHORYLATION;

RAPTORES;

AMINO ACIDS; CELL LINE; CELL NUCLEUS; CYTOPLASM; DIMERIZATION; GUANOSINE TRIPHOSPHATE; HUMANS; INSULIN; LEUCINE; MONOMERIC GTP-BINDING PROTEINS; MUTANT PROTEINS; MUTATION; NEUROPEPTIDES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN KINASES; PROTEINS; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS;

EID: 45849105156     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1157535     Document Type: Article

References (30)

1. D. A. Guertin, D. M. Sabatini, Cancer Cell 12, 9 (2007).
2. D. D. Sarbassov, S. M. Ali, D. M. Sabatini, Curr. Opin. Cell Biol. 17, 596 (2005).
3. M. P. Byfield, J. T. Murray, J. M. Backer, J. Biol. Chem. 280, 33076 (2005).
4. T. Nobukuni et al., Proc. Natl. Acad. Sci. U.S.A. 102, 14238 (2005).
5. E. M. Smith, S. G. Finn, A. R. Tee, G. J. Browne, C. G. Proud, J. Biol. Chem. 280, 18717 (2005).
6. Materials and methods are available as supporting material on Science Online.
7. D.-H. Kim et al., Cell 110, 163 (2002).
8. R. Loewith et al., Mol. Cell 10, 457 (2002).
9. K. P. Wedaman et al., Mol. Biol. Cell 14, 1204 (2003).
10. K. Hara et al., Cell 110, 177 (2002).
11. M. Bun-Ya, S. Harashima, Y. Oshima, Mol. Cell. Biol. 12, 2958 (1992).
12. A. Schurmann, A. Brauers, S. Massmann, W. Becker, H. G. Joost, J. Biol. Chem. 270, 28982 (1995).
13. E. Hirose, N. Nakashima, T. Sekiguchi, T. Nishimoto, J. Cell Sci. 111, 11 (1998).
14. N. Nakashima, E. Noguchi, T. Nishimoto, Genetics 152, 853 (1999).
15. T. Sekiguchi, E. Hirose, N. Nakashima, M. Ii, T. Nishimoto, J. Biol. Chem. 276, 7246 (2001).
16. M. Gao, C. A. Kaiser, Nat. Cell Biol. 8, 657 (2006).
17. F. Dubouloz, O. Deloche, V. Wanke, E. Cameroni, C. De Virgilio, Mol. Cell 19, 15 (2005).
18. J. B. Kunz, H. Schwarz, A. Mayer, J. Biol. Chem. 279, 9987 (2004).
19. K. J. Roberg, N. Rowley, C. A. Kaiser, J. Cell Biol. 137, 1469 (1997).
20. E. J. Chen, C. A. Kaiser, J. Cell Biol. 161, 333 (2003).
21. A. Beugnet, A. R. Tee, P. M. Taylor, C. G. Proud, Biochem. J. 372, 555 (2003).
22. G. R. Christie, E. Hajduch, H. S. Hundal, C. G. Proud, P. M. Taylor, J. Biol. Chem. 277, 9952 (2002).
23. D. J. Price, R. A. Nemenoff, J. Avruch, J. Biol. Chem. 264, 13825 (1989).
24. Y. Sancak et al., Mol. Cell 25, 903 (2007).
25. X. Long, Y. Lin, S. Ortiz-Vega, K. Yonezawa, J. Avruch, Curr. Biol. 15, 702 (2005).
26. R. M. Drenan, X. Liu, P. G. Bertram, X. F. Zheng, J. Biol. Chem. 279, 772 (2004).
27. M. Mavrakis, J. Lippincott-Schwartz, C. A. Stratakis, I. Bossis, Autophagy 3, 151 (2007).
28. K. Saito, Y. Araki, K. Kontani, H. Nishina, T. Katada, J. Biochem. 137, 423 (2005).
29. C. Buerger, B. DeVries, V. Stambolic, Biochem. Biophys. Res. Commun. 344, 869 (2006).
30. Supported by grants from the NIH (R01 CA103866 and AI47389), Department of Defense (W81XWH-07-0448), and W.M. Keck Foundation to D.M.S. as well as an EMBO fellowship to Y.D.S. We thank T. Kang for the preparation of FLAG-raptor and members of the Sabatini lab for helpful suggestions.