Fingers-closing and other rapid conformational changes in DNA polymerase I (Klenow fragment) and their role in nucleotide selectivity

Biochemistry

Volumn 47, Issue 23, 2008, Pages 6103-6116

  Joyce, Catherine M ^a   Potapova, Olga ^a   DeLucia, Angela M ^a,b   Huang, Xuanwei ^a,c   Basu, Vandana Purohit ^a   Grindley, Nigel D F ^a  

^a YALE UNIVERSITY   (United States)

^b NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

^c Barr Pharmaceuticals Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CALCIUM; DNA; EXPERIMENTS; GENES; NUCLEIC ACIDS; NUCLEOTIDES; ORGANIC ACIDS;

BINARY COMPLEXES; CHEMICAL QUENCH EXPERIMENTS; CONFORMATIONAL CHANGES; CONFORMATIONAL TRANSITIONS; DNA POLYMERASES; FLUORESCENCE ASSAYS; KINETIC STUDIES; KLENOW FRAGMENT (KF); RATE LIMITING; REACTION PATHWAYS;

RATE CONSTANTS;

CALCIUM ION; DEOXYRIBONUCLEOTIDE; DNA DIRECTED DNA POLYMERASE BETA; DNA FRAGMENT; MAGNESIUM ION; METAL ION; NUCLEOTIDE; PARATHION; PRIMER DNA; RIBONUCLEOTIDE;

ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; DNA BINDING; DNA DEGRADATION; DNA TEMPLATE; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME BINDING; ENZYME CONFORMATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; REACTION ANALYSIS;

CRYSTALLOGRAPHY, X-RAY; DNA; DNA POLYMERASE I; KINETICS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; PEPTIDE FRAGMENTS; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; TRANSCRIPTION, GENETIC;

EID: 44949114390     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi7021848     Document Type: Article

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