Obtainment and characterization of the evolved enzymes from Arabidopsis thaliana glutathione S-transferase zeta class

Progress in Biochemistry and Biophysics

Volumn 35, Issue 2, 2008, Pages 208-216

  Tao, Su Dan ^a   Chen, Xi Wen ^a   Liu, Jia ^a   Jia, Xiang Dong ^a   Chen, De Fu ^a  

^a NANKAI UNIVERSITY   (China)

Author keywords

Arabidopsis thaliana glutathione S transferases zeta class (AiGSTZ); Dichloroacetic acid dechlorination (DCA DC) activity; Evolved mutants; Renaturability; Thermostability

Indexed keywords

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 44449146958     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Armstrong R N. Structure, catalytic mechanism, and evolution of the glutathione transferases. Chem Res Toxicol, 1997, 10(1): 2-18
2. Dixon D P, Lapthorn A, Edwards R. Plant glutathione transferases. Genome Biol, 2002, 3(3): 1-10
3. Edwards R. Characterisation of glutathione transferases and glutathione peroxidases in pea (Pisum sativum). Physiol Plant, 1996, 98(3): 594-604
4. Dixon D P, Cole D J, Edwards R. Characterisation of a zeta class glutathione transferase from Arabidopsis thaliana with a putative role in tyrosine catabolism. Arch Biochem Biophys, 2000, 384(2): 407-412
5. Thorn R, Dixon D P, Edwards R. The structure of a zeta class glutathione 5-transferase from Arabidopsis thaliana: characterisation of a GST with novel active-site architecture and a putative role in tyrosine catabolism. J Mol Biol, 2001, 308(5): 949-962
6. Zeng Q Y, Wang X R. Catalytic properties of glutathione-binding residues in a tau class glutathione transferase (PtGSTU1) from Pinus tabulaeformis. FEBS Lett, 2005, 579(12): 2657-2662
7. Winayanuwattikun P, Ketterman A J. Catalytic and structural contributions for glutathione-binding residues in a delta class glutathione S-transferase. Biochem J, 2004, 382(Pt 2): 751-757
8. Wongsantichon J, Ketterman A J. An intersubunit lock-and-key 'clasp' motif in the dimer interface of delta class glutathione transferase. Biochem J, 2006, 394(Pt 1): 135-144
9. Tong Z, Board P G, Anders M W. Glutathione transferase zeta catalyses the oxygenation of the carcinogen dichloroacetic acid to glyoxylic acid. Biochem J, 1998,331(pt2): 371-374
10. Ricci G, Turella P, De Maria F, et al. Binding and kinetic mechanisms of the zeta class glutathione transferase. J Biol Chem, 2004, 279(32): 33336-33342
11. Anderson W B, Board P G, Anders M W. Glutathione transferase zeta-catalyzed bioactivation of dichloroacetic acid: reaction of glyoxylate with amino acid nucleophiles. Chem Res Toxicol, 2004, 17(5): 650-662
12. You L, Arnold F H. Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide. Protein Eng, 1996, 9(1): 77-83
13. Cirino P C, Mayer K M, Umeno D. Generating mutant libraries using error-prone PCR. In: Arnold F H, Georgiou G, eds. Methods in Molecular Biology 231-Directed Evolution Library Creation. Totowa, NJ: Humana Press Inc, 2003. 3-10
14. Joem J M. DNA shuffling. In: Arnold F H, Georgiou G, eds. Methods in Molecular Biology, 231-Directed Evolution Library Creation. Totowa, NJ: Humana Press Inc, 2003. 85-90
15. Holloway P, Trevors J T, Lee H. A colorimetric assay for detecting haloalkane dehalogenase activity. J Microbiol Methods, 1998, 32(1): 31-36
16. Chen D, Kawarasaki Y, Nakano H, et al. Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genes. J Biosci Bioeng, 2003, 95(6): 594-600
17. Lee P C, Petri R, Mijts B N, et al. Directed evolution of Escherichia coli farnesyl diphosphate synthase (IspA) reveals novel structural determinants of chain length specificity. Metab Eng, 2005, 7(1): 18-26
18. Sclimenti C R, Thyagarajan B, Calos M P. Directed evolution of a recombinase for improved genomic integration at a native human sequence. Nucleic Acids Res, 2001, 29(24): 5044-5051
19. Guo H H, Choe J, Loeb L A. Protein tolerance to random amino acid change. Proc Natl Acad Sci USA, 2004, 101(25): 9205-9210
20. Bloom J D, Meyer M M, Meinhold P, et al. Evolving strategies for enzyme engineering. Curr Opin Strucl Biol, 2005, 15(4): 447-452
21. Matsumura I, Ellington A D. In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates. J Mol Biol, 2001, 305(2): 331-339