Small interfering RNA-induced transcriptional gene silencing in human cells

Science

Volumn 305, Issue 5688, 2004, Pages 1289-1292

  Morris, Kevin V ^a,d   Chan, Simon W L ^b   Jacobsen, Steven E ^b   Looney, David J ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c VA SAN DIEGO HEALTHCARE SYSTEM   (United States)

^d CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; PROTEINS; RNA; TISSUE;

PERMEABITIZATION; POSTTRANSCRIPTIONAL; TISSUE CULTURE CELLS; TRANSDUCTION;

GENES;

DNA; ELONGATION FACTOR 1ALPHA; SMALL INTERFERING RNA;

BIOLOGY; RNA;

ARTICLE; DNA METHYLATION; GENE FUNCTION; GENE SILENCING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; LENTIVIRINAE; MEMBRANE PERMEABILITY; PRIORITY JOURNAL; PROMOTER REGION; RNA TRANSLATION; RNA TRANSPORT; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION;

ACTIVE TRANSPORT, CELL NUCLEUS; CELL LINE; CELL NUCLEUS; DNA METHYLATION; DNA-BINDING PROTEINS; DOWN-REGULATION; EUKARYOTIC INITIATION FACTOR-1; GENE SILENCING; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNODEFICIENCY VIRUS, FELINE; LUMINESCENT PROTEINS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; TRANSCRIPTION, GENETIC; TRANSDUCTION, GENETIC; TRANSFECTION; TRANSGENES;

LENTIVIRUS; MAMMALIA;

EID: 4344705410     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1101372     Document Type: Article

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32. We thank C. Chung and W. Witke for technical support and K. Susuki for comments regarding transcriptional gene silencing. S.W-L.C is a U.S. Department of Energy (DOE) Energy Biosciences fellow of the Life Sciences Research Foundation Supported by NIH AIDS training grant no. AI07384, NIH grant no. P01 AI45992 (D.J.L.), the UCSD Center for AIDS Research, NIH grant no. P30 AI36214, and a Beckman Young Investigator Award (S.E J.).