Stabilization of antibody structure upon association to a human carbonic anhydrase IX epitope studied by X-ray crystallography, microcalorimetry, and molecular dynamics simulations

Proteins: Structure, Function and Genetics

Volumn 71, Issue 3, 2008, Pages 1275-1287

  Král, Vlastimil ^a,b   Mader, Pavel ^a,b   Collard, Renata ^a,d   Fábry, Milan ^a   Hořejší, Magdaléna ^a   Řezáčová, Pavlína ^a   Kožíšek, Milan ^b,c   Závada, Jan ^c   Sedláček, Juraj ^a   Rulíšek, Lubomír ^c   Brynda, Jiří ^a,c  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

^c INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY   (Czech Republic)

^d UNIVERSITY OF COLORADO   (United States)

Author keywords

Antigen antibody recognition; CDR loop rearrangement; Fab crystal structure; Microcalorimetry, MM GBSA calculations; TLS method

Indexed keywords

ANTIBODY; CARBONATE DEHYDRATASE IX;

ANTIBODY STRUCTURE; ANTIGEN ANTIBODY REACTION; ARTICLE; CRYSTAL STRUCTURE; ENTHALPY; ISOTHERMAL TITRATION CALORIMETRY; LIGHT SCATTERING; MICROCALORIMETRY; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN DOMAIN; SIMULATION; THERMODYNAMICS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ANTIGENS, NEOPLASM; BINDING SITES, ANTIBODY; CALORIMETRY; CARBONIC ANHYDRASES; CELL LINE, TUMOR; COMPUTER SIMULATION; CRYSTALLOGRAPHY, X-RAY; EPITOPES; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; ISOENZYMES; MOLECULAR SEQUENCE DATA; THERMODYNAMICS;

EID: 42449122268     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.21821     Document Type: Article

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