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DNA was synthesized using standard phosphoramidite chemistry and characterized by MALDI-TOF mass spectrometry, UV-visible spectroscopy, and HPLC The 5′c C6 Thiol Modifier S-S and NHS-Carboxy-dT phosphoramidites were purchased from Glen Research. On solid support, NB was coupled to the 5-[3-acrylate NHS Ester]-deoxy uridine to form an amide bond. See Supporting Information for the NB coupling and characterization
-
DNA was synthesized using standard phosphoramidite chemistry and characterized by MALDI-TOF mass spectrometry, UV-visible spectroscopy, and HPLC The 5′c C6 Thiol Modifier S-S and NHS-Carboxy-dT phosphoramidites were purchased from Glen Research. On solid support, NB was coupled to the 5-[3-acrylate NHS Ester]-deoxy uridine to form an amide bond. See Supporting Information for the NB coupling and characterization.
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Significant hypochromicity of the NB chromophore is seen in the NB-DNA conjugate which likely indicates that NB is stacked in the duplex Supporting Information, For further spectroscopic characterization of the interaction of free NB with DNA, see: Lakowicz, J. R, Piszczek, G, Kang, J. S. Anal. Biochem. 2001, 288, 62
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Significant hypochromicity of the NB chromophore is seen in the NB-DNA conjugate which likely indicates that NB is stacked in the duplex (Supporting Information). For further spectroscopic characterization of the interaction of free NB with DNA, see: Lakowicz, J. R.; Piszczek, G.; Kang, J. S. Anal. Biochem. 2001, 288, 62.
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We have found that the midpoint potential of NB-DNA monolayers on macroelectrodes vary depending upon the gold surface utilized, leading to an uncertainty of ∼50 mV in the potentials, although they have a higher certainty within a given surface. Moreover, there is a systematic negative shift in potential of ∼100 mV on the microelectrodes versus the macroelectrodes. Comparable shifts have been seen by others (see ref 18 b,c) and may reflect the different geometries of DNA monolayers on the different surfaces. See Supporting Information for further details
-
We have found that the midpoint potential of NB-DNA monolayers on macroelectrodes vary depending upon the gold surface utilized, leading to an uncertainty of ∼50 mV in the potentials, although they have a higher certainty within a given surface. Moreover, there is a systematic negative shift in potential of ∼100 mV on the microelectrodes versus the macroelectrodes. Comparable shifts have been seen by others (see ref 18 b,c) and may reflect the different geometries of DNA monolayers on the different surfaces. See Supporting Information for further details.
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At small scales, we observe a partially sigmoidal shape for the voltammetry of Nile Blue-DNA films. We attribute the sigmoidal shape to a small catalytic contribution to the NB signal from trace oxygen, as reported in Ju, H, Shen, C. Electroanalysis 2001, 13, 789. See Supporting Information
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At small scales, we observe a partially sigmoidal shape for the voltammetry of Nile Blue-DNA films. We attribute the sigmoidal shape to a small catalytic contribution to the NB signal from trace oxygen, as reported in Ju, H.; Shen, C. Electroanalysis 2001, 13, 789. See Supporting Information.
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See Supporting Information for details of protein preparation.
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See Supporting Information for details of protein preparation.
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