Direct electrochemistry of endonuclease III in the presence and absence of DNA

Journal of the American Chemical Society

Volumn 128, Issue 37, 2006, Pages 12082-12083

  Gorodetsky, Alon A ^a   Boal, Amie K ^a   Barton, Jacqueline K ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BASE EXCISION REPAIR PROTEIN; DEOXYRIBONUCLEOPROTEIN; DNA; ENDONUCLEASE; ENDONUCLEASE III; GRAPHITE; UNCLASSIFIED DRUG;

ARTICLE; DNA SEQUENCE; ELECTROCHEMICAL ANALYSIS; ELECTROCHEMISTRY; ELECTRODE; ENZYME ACTIVITY; EXCISION REPAIR; OXIDATION REDUCTION REACTION; PROTEIN DNA BINDING; PROTEIN FOLDING; PROTEIN FUNCTION;

DEOXYRIBONUCLEASES, TYPE III SITE-SPECIFIC; DNA; ELECTROCHEMISTRY; ELECTRODES; GRAPHITE; NUCLEIC ACID CONFORMATION; OXIDATION-REDUCTION;

EID: 33748790129     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja064784d     Document Type: Article

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43. 3 controls.
44. The potential difference between 3+/2+and 2+/1 +couples is somewhat smaller than expected (ref 5) and may be an underestimate since we are on the edge of the potential window.
45. SWV gives a shift of 280 mV between the cathodic DNA-bound potential and the anodic potential on bare HOPG. The shift in midpoint potentials should be slightly smaller. Given the lack of reversibility, we provide only a lower estimate in the shift.