Enhanced micronucleus formation and modulation of Bcl-2: Bax in MCF-7 cells after exposure to binary mixtures

Environmental Health Perspectives

Volumn 115, Issue SUPPL1, 2007, Pages 129-136

  Hewitt, Rebecca ^a   Forero, Albert ^a   Luncsford, Paz J ^a   Martin, Francis L ^a  

^a LANCASTER UNIVERSITY   (United Kingdom)

Author keywords

17 estradiol; BAX; Bcl 2; Benzo a pyrene; Binary mixture; Clonogenic assay; Cytokinesis block micronucleus assay; Lindane; Micronucleus; PhIP

Indexed keywords

2 AMINO 1 METHYL 6 PHENYLIMIDAZO(4,5 B)PYRIDINE; 2-AMINO-1-METHYL-6-PHENYLIMIDAZO(4,5-B)PYRIDINE; BENZO[A]PYRENE; CYCLIN DEPENDENT KINASE INHIBITOR 1A; CYTOCHROME P450; ESTRADIOL; IMIDAZOLE DERIVATIVE; LINDANE; PROTEIN BAX; PROTEIN BCL 2; UNCLASSIFIED DRUG; XENOBIOTIC AGENT;

ARTICLE; BREAST TUMOR; DRUG EFFECT; DRUG INTERACTION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; METABOLISM; MICRONUCLEUS; MICRONUCLEUS TEST; TUMOR CELL LINE;

BCL-2-ASSOCIATED X PROTEIN; BENZO(A)PYRENE; BREAST NEOPLASMS; CELL LINE, TUMOR; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYTOCHROME P-450 ENZYME SYSTEM; DRUG INTERACTIONS; ESTRADIOL; GENE EXPRESSION REGULATION; HUMANS; IMIDAZOLES; LINDANE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; PROTO-ONCOGENE PROTEINS C-BCL-2; XENOBIOTICS;

EID: 40949153125     PISSN: 00916765     EISSN: 15529924     Source Type: Journal    
DOI: 10.1289/ehp.9361     Document Type: Article

References (48)

1. Adams JM, Cory S. 1998. The Bcl-2 protein family: arbiters of cell survival. Science 281:1322-1326.
2. Cheng SC, Hilton BD, Roman JM, Dipple A. 1989. DNA adducts from carcinogenic and noncarcinogenic enantiomers of benzo[a]pyrene dihydrodiol epoxide. Chem Res Toxicol 2:334-340.
3. Cranmer M, Louie S, Kennedy RH, Kern PA, Fonseca VA. 2000. Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is associated with hyperinsulinemia and insulin resistance. Toxicol Sci 56:431-436.
4. Creton S, Zhu H, Gooderham NJ. 2005. A mechanistic basis for the role of cycle arrest in the genetic toxicology of the dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Toxicol Sci 84:335-343.
5. Crofts FG, Sutter TR, Strickland PT. 1998. Metabolism of 2-amino-1- methyl-6-phenylimidazo[4,5-b]pyridine by human cytochrome P4501A1, P4501A2 and P4501B1. Carcinogenesis 19:1969-1973.
6. Culp SJ, Warbritton AR, Smith BA, Li EE, Beland FA. 2000. DNA adduct measurements, cell proliferation and tumor mutation induction in relation to tumor formation in B6C3F1 mice fed coal tar or benzo[a]pyrene. Carcinogenesis 21:1433-1440.
7. Davis C, Bhana S, Shorrocks AJ, Martin FL. 2002. Oestrogens induce G1 arrest in benzo[a]pyrene-treated MCF-7 breast cells whilst enhancing genotoxicity and clonogenic survival. Mutagenesis 17:431-438.
8. Döhr O, Vogel C, Abel J. 1995. Different response of 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD)-sensitive genes in human breast cancer MCF-7 and MDA-MB 231 cells. Arch Biochem Biophys 321:405-412.
9. Eltom SE, Larsen MC, Jefcoate CR. 1998. Expression of CYP1B1 but not CYP1A1 by primary cultured human mammary stromal fibroblasts constitutively and in response to dioxin exposure: role of the Ah receptor. Carcinogenesis 19:1437-1444.
10. Felton JS, Knize MG, Bennett LM, Malfatti MA, Colvin ME, Kulp KS. 2004. Impact of environmental exposures on the mutagenicity/ carcinogenicity of heterocyclic amines. Toxicology 198:135-145.
11. GenBank. 2006. Home Page. Bethesda, MD:National Center for Biotechnology Information. Available: http://www.ncbi.nlm.nih.gov/ [accessed 24 July 2006].
12. Gooderham NJ, Zhu H, Lauber S, Boyce A, Creton S. 2002. Molecular and genetic toxicology of 2-amino-1-methyl-6- phenylimidazo[4,5-b]pyridine (PhIP). Mutat Res 506-507: 91-99.
13. Grover PL, Martin FL. 2002. The initiation of breast and prostate cancer. Carcinogenesis 23:1095-1102.
14. Jeffy BD, Chirnomas RB, Romagnolo DF. 2002. Epigenetics of breast cancer: polycyclic aromatic hydrocarbons as risk factors. Environ Mol Mutagen 39:235-244.
15. Jiao H, Allinson SL, Walsh MJ, Hewitt R, Cole KJ, Phillips DH, Martin FL. 2007. Growth kinetics in MCF-7 cells modulate benzo[a]pyrene-induced CYP1A1 up-regulation. Mutagenesis 22:111-116.
16. Joosten HF, van Acker FA, van den Dobbelsteen DJ, Horbach GJ, Krajnc EI. 2004. Genotoxicity of hormonal steroids. Toxicol Lett 151:113-134; doi:10.1016/j.toxlet.2004.01.018.
17. Kalantzi OI, Hewitt R, Ford KJ, Alcock RE, Thomas GO, Morris JA, et al. 2004a. Inter-individual differences in the ability of human milk-fat extracts to enhance the genotoxic potential of the procarcinogen benzo[a]pyrene in MCF-7 breast cells. Environ Sci Technol 38:3614-3622.
18. Kalantzi OI, Hewitt R, Ford KJ, Cooper L, Alcock RE, Thomas GO, et al. 2004b. Low dose induction of micronuclei by lindane. Carcinogenesis 25:613-622.
19. Kalantzi OI, Martin FL, Thomas GO, Alcock RE, Tang HR, Drury SC, et al. 2004c. Different levels of polybrominated diphenyl ethers (PBDEs) and chlorinated compounds in breast milk from two U.K. regions. Environ Health Perspect 112: 1085-1091.
20. Kamel F, Hoppin JA. 2004. Association of pesticide exposure with neurologic dysfunction and disease. Environ Health Perspect 112:950-958.
21. Kaspin LC, Baird WM. 1996. Anti-benzo[a]pyrene-7,8-diol-9,10- epoxide treatment increases levels of proteins p53 and p21WAF1 in the human carcinoma cell line MCF-7. Polycyclic Aromatic Compounds 10:299-306.
22. Lauber SN, Ali S, Gooderham NJ. 2004. The cooked food derived carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5- b] pyridine is a potent oestrogen: a mechanistic basis for its tissue-specific carcinogenicity. Carcinogenesis 25:2509-2517.
23. Leung LK, Wang TT. 1999. Paradoxical regulation of Bcl-2 family proteins by 17β-oestradiol in human breast cancer cells MCF-7. Br J Cancer 81:387-392.
24. Maier A, Schumann BL, Chang X, Talaska G, Puga A. 2002. Arsenic co-exposure potentiates benzo[a]pyrene genotoxicity. Mutat Res 517:101-111.
25. Marston CP, Pereira C, Ferguson J, Fischer K, Hedstrom O, Dashwood W, et al. 2001. Effect of a complex environmental mixture from coal tar containing polycyclic aromatic hydrocarbons (PAH) on the tumor initiation, PAH-DNA binding and metabolic activation of carcinogenic PAH in mouse epidermis. Carcinogenesis 22:1077-1086.
26. Martin FL, Semple KT. 2004. Environmental health impacts: occurrence, exposure and significance, Lancaster University, UK, 9-10 September 2003. Mutagenesis 19:423-429.
27. McLuckie KIE, Gaskell M, Farmer PB, Martin EA, Jones GDD, Routledge MN. 2004. Effects of the order of exposure to a binary mixture of mutagens on the induced mutation spectra in the supF gene. Mutagenesis 19:137-141.
28. Moggs JG, Orphanides G. 2001. Estrogen receptors: orchestrators of pleiotropic cellular responses. EMBO Reports 2:775-781.
29. Mueller SO, Simon S, Chae K, Metzler M, Korach KS. 2004. Phytoestrogens and their human metabolites show distinct agonistic and antagonistic properties on estrogen receptor alpha (ER) and ERβ in human cells. Toxicol Sci 80:14-25.
30. Oikawa K, Ohbayashi T, Mimura J, Iwata R, Kameta A, Evine K, et al. 2001. Dioxin suppresses the checkpoint protein, MAD2, by aryl hydrocarbon receptor-independent pathway. Cancer Res 61:5707-5709.
31. Peto J. 2001. Cancer epidemiology in the last century and the next decade. Nature 411:390-395.
32. Plíšková M, Vondráček J, Vojtěšek B, Kozubík A, Machala M. 2004. Deregulation of cell proliferation by polycyclic aromatic hydrocarbons in human breast carcinoma MCF-7 cells reflects both genotoxic and nongenotoxic events. Toxicol Sci 83:246-256.
33. Ragavan N, Grover PL, Balasubramanian SP, Hindley AC, Matanhelia SS, Martin FL. 2006. An observational study of cancers among female partners of UK-resident prostate cancer patients. Cancer Lett 242:88-94.
34. Ragavan N, Hewitt R, Cooper LJ, Ashton KM, Hindley AC, Nicholson CM, et al. 2004. CYP1B1 expression in prostate is higher in the peripheral than in the transition zone. Cancer Lett 215:69-78.
35. Rajapakse N, Butterworth M, Kortenkamp A. 2005. Detection of DNA strand breaks and oxidized DNA bases at the singlecell level resulting from exposure to estradiol and hydroxylated metabolites. Environ Mol Mutagen 45:397-404.
36. Rubin H. 2001. Synergistic mechanisms in carcinogenesis by polycyclic aromatic hydrocarbons and by tobacco smoke: a bio-historical perspective with updates. Carcinogenesis 22:1903-1930.
37. Safe S. 2004. Endocrine disruptors and human health: is there a problem. Toxicology 205:3-10.
38. Schut HA, Snyderwine EG. 1999. DNA adducts of heterocyclic amine food mutagens: implications for mutagenesis and carcinogenesis. Carcinogenesis 20:353-368.
39. Sims P, Grover PL, Swaisland A, Pal K, Hewer A. 1974. Metabolic activation of benzo(a)pyrene proceeds by a diol-epoxide. Nature 252:326-328.
40. Slaga TJ, Jecker L, Braken WM, Weeks CE. 1979. The effects of weak or non-carcinogenic polycyclic hydrocarbons on 7,12-dimethylbenz[a]anthracene and benzo[a]pyrene skin tumor-initiation. Cancer Lett 7:51-59.
41. Theodorakis P, Lomonosova E, Chinnadurai G. 2002. Critical requirement of BAX for manifestation of apoptosis induced by multiple stimuli in human epithelial cancer cells. Cancer Res 62:3373-3376.
42. Vaux DL, Korsmeyer SJ. 1999. Cell death in development. Cell 96:245-254.
43. Venugopal M, Callaway A, Snyderwine EG. 1999. 2-Amino-1- methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) retards mammary gland involution in lactating Sprague-Dawley rats. Carcinogenesis 20:1309-1314.
44. Wang W, Smith R, Safe S. 1998. Aryl hydrocarbon receptormediated antiestrogenicity in MCF-7 cells: modulation of hormone-induced cell cycle enzymes. Arch Biochem Biophys 356:239-248.
45. Welshons WV, Thayer KA, Judy BM, Taylor JA, Curran EM, vom Saal FS. 2003. Large effects from small exposures. I. Mechanisms for endocrine-disrupting chemicals with estrogenic activity. Environ Health Perspect 111:994-1006.
46. Yared E, McMillan TJ, Martin FL. 2002. Genotoxic effects of oestrogens in breast cells detected by the micronucleus and the Comet assay. Mutagenesis 17:345-352.
47. Zhang YJ, Weksler BB, Wang L, Schwartz J, Santella RM. 1998. Immunohistochemical detection of polycyclic aromatic hydrocarbon-DNA damage in human blood vessels of smokers and non-smokers. Atherosclerosis 140:325-331.
48. Zhao K, Murray S, Davies DS, Boobis AR, Gooderham NJ. 1994. Metabolism of the food derived mutagen and carcinogen 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) by human liver microsomes. Carcinogenesis 15:1285-1288.