Mesenchymal stem cell tissue engineering: Techniques for isolation, expansion and application

Injury

Volumn 38, Issue 4 SUPPL., 2007, Pages

  Pountos, Ippokratis ^a   Corscadden, Diane ^a   Emery, Paul ^a   Giannoudis, Peter V ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Bone repair; Culture; Implantation; Mesenchymal stem cells

Indexed keywords

CELL ADHESION MOLECULE; CYTOKINE; GROWTH FACTOR; INTEGRIN; LYMPHOCYTE ANTIGEN; SCAFFOLD PROTEIN;

APOPTOSIS; ARTICLE; BIOREACTOR; BONE MARROW CELL; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL PROLIFERATION; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ISOLATION; EX VIVO STUDY; HUMAN; MESENCHYMAL STEM CELL; PHENOTYPE; PRIORITY JOURNAL; TISSUE ENGINEERING;

ANIMALS; BONE DISEASES; BONE SUBSTITUTES; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; FRACTURES, BONE; HUMANS; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STEM CELLS; TISSUE ENGINEERING; TREATMENT OUTCOME;

EID: 38549090983     PISSN: 00201383     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0020-1383(08)70006-8     Document Type: Article

References (125)

1. Abdel-Hamid M., Hussein M., Ahmad A., and Elgezawi E. Enhancement of the repair of meniscal wounds in the red-white zone (middle third) by the injection of bone marrow cells in canine animal model. Int J Exp Pathol 86 (2005) 117-123
2. Arinzeh T., Peter S., Archambault M., et al. Allogeneic mesenchymal stem cells regenerate bone in a criticalsized canine segmental defect. J Bone Joint Surg Am 85A (2003) 1927-1935
3. Banfi A., Muraglia A., Dozin B., et al. Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: Implications for their use in cell therapy. Exp Hematol 28 (2000) 707-715
4. Barbash I., Chouraqui P., Baron J., et al. Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium: feasibility, cell migration, and body distribution. Circulation 108 (2003) 863-868
5. Baumgarth N., and Roederer M. A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 243 (2000) 77-97
6. Bensaid W., Triffitt J., Blanchat C., et al. A biodegradable fibrin scaffold for mesenchymal stem cell transplantation. Biomaterials 24 (2003) 2497-2502
7. Benya P., and Shaffer J. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30 (1982) 215-224
8. Braccini A., Wendt D., Jaquiery C., et al. Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts. Stem Cells 23 (2005) 1066-1072
9. Brekke J., and Toth J. Principles of tissue engineering applied to programmable osteogenesis. J Biomed Mater Res 43 (1998) 380-398
10. Brodie J., Goldie E., Connel G., Merry J., and Grant M. Osteoblast interactions with calcium phosphate ceramics modified by coating with type I collagen. J Biomed Mater Res A 73 (2005) 409-421
11. Buhring H., Battula V., Treml S., et al. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci (2007) [Epub ahead of print].
12. Cai X., Lin Y., Ou G., et al. Ectopic osteogenesis and chondrogenesis of bone marrow stromal stem cells in alginate system. Cell Biol Int (2007) [Epub ahead of print].
13. Centeno C., Kisiday J., Freeman M., and Schultz J. Partial regeneration of the human hip via autologous bone marrow nucleated cell transfer: A case study. Pain Physician 9 (2006) 253-256
14. Chachques J., Herreros J., Trainini J., et al. Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular cardiomyo-plasty. Int J Cardiol 95 (2004) S29-S33
15. Chang C., Stanton R., and Glutting J. Unicameral bone cysts treated by injection of bone marrow or methylprednisolone. J Bone Joint Surg Br 84 (2002) 407-412
16. Chen D., Zeng H., Du S., et al. Extracts from Plastrum testudinis promote proliferation of rat bone-marrow-derived mesenchymal stem cells. Cell Prolif 40 (2007) 196-212
17. Chen F., Mao T., Tao K., et al. Bone graft in the shape of human mandibular condyle reconstruction via seeding marrow-derived osteoblasts into porous coral in a nude mice model. J Oral Maxillofac Surg 60 (2002) 1155-1159
18. Chen S., Fang W., Ye F., et al. Effect on left ventricular function of intracoronary transplantation of autologous bone marrow mesenchymal stem cell in patients with acute myocardial infarction. Am J Cardiol 94 (2004) 92-95
19. Cho B., Kim T., Yang J., et al. Effect of calcium sulfate-chitosan composite: pellet on bone formation in bone defect. J Craniofac Surg 16 (2005) 213-224
20. Choi Y., Park S., and Suh H. Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres. Biomaterials 26 (2005) 5855-5863
21. Connolly J. Injectable bone marrow preparations to stimulate osteogenic repair. Clin Orthop 313 (1995) 8-18
22. Crisostomo P., Wang M., Wairiuko G., et al. High passage number of stem cells adversely affects stem cell activation and myocardial protection. Shock 26 (2006) 575-580
23. Cristino S., Grassi F., Toneguzzi S., et al. Analysis of mesenchymal stem cells grown on a three-dimensional HYAFF 11-based prototype ligament scaffold. J Biomed Mater Res A 73 (2005) 275-283
24. Deans R., and Moseley A. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol 28 (2000) 875-884
25. Devine M., Mierisch C., Jang E., et al. Transplanted bone marrow cells localize to fracture callus in a mouse model. J Orthop Res 20 (2002) 1232-1239
26. Devine S. Mesenchymal stem cells: will they have a role in the clinic?. J Cell Biochem Suppl 38 (2002) 73-79
27. Dietel M., Arps H., Gerding D., Trapp M., and Niendorf A. Establishment of primary cell cultures: experiences with 155 cell strains. Klin Wochenschr 65 (1987) 507-512
28. Digirolamo C., Stokes D., Colter D., et al. Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br J Haematol 107 (1999) 275-281
29. Eagle H. Nutrition Needs of Mammalian Cells in Culture. Science 122 (1955) 501-514
30. Edwards S., Mitchell W., Matthews J., et al. Design of nonwoven scaffold structures for tissue engineering of the anterior cruciate ligament. Research Journal 4 (2004) 86-94
31. Fickert S., Fiedler J., and Brenner R. Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers. Arthritis Res Ther 6 (2004) R422-R432
32. Frenkel S., Toolan B., Menche D., et al. Chondrocyte transplantation using a collagen bilayer matrix for cartilage repair. J Bone Joint Surg Br 79 (1997) 831-836
33. Friedenstein A., Gorskaja J., and Kulagina N. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 4 (1976) 267-274
34. Gronthos S., and Simmons P. The growth factor requirements of STRO-1-positive human bone marrow stromal precursors under serum-deprived conditions in vitro. Blood 85 (1995) 929-940
35. Guo X., Du J., Zheng Q., et al. Molecular tissue engineering: applications for modulation of mesenchymal stem cells proliferation by transforming growth factor beta 1 gene transfer. J Tongji Med Univ 21 (2001) 314-317
36. Guo Z., Li H., Li X., et al. In vitro characteristics and in vivo immunosuppressive activity of compact bone-derived murine mesenchymal progenitor cells. Stem Cells 24 (2006) 992-1000
37. Hankey D., McCabe R., Doherty M., et al. Enhancement of human osteoblast proliferation and phenotypic expression when cultured in human serum. Acta Orthop Scand 72 (2001) 395-403
38. Hasegawa S., Neo M., Tamura J., et al. In vivo evaluation of a porous hydroxyapatite/poly-DL-lactide composite for bone tissue engineering. J Biomed Mater Res A 81A (2007) 930-938
39. Hernigou P., and Beaujean F. Treatment of osteonecrosis with autologous bone marrow grafting. Clin Orthop Relat Res 405 (2002) 14-23
40. Hernigou P., Poignard A., Beaujean F., and Rouard H. Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells. J Bone Joint Surg Am 87 (2005) 1430-1437
41. Hou R., Chen F., Yang Y., et al. Comparative study between coral-mesenchymal stem cells-rhBMP-2 composite and auto-bone-graft in rabbit critical-sized cranial defect model. J Biomed Mater Res A 80 (2007) 85-93
42. Hunziker E. Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. Osteoarthritis Cartilage 10 (2002) 432-463
43. nd sensor symposium, Oct. 20-21, 2005, Tokyo, pp 125-128.
44. Jorgensen C., Gordeladze J., and Noel D. Tissue engineering through autologous mesenchymal stem cells. Curr Opin Biotechnol 15 (2004) 406-410
45. Khanal G., Garg M., and Singh G. A prospective randomized trial of percutaneous marrow injection in a series of closed fresh tibial fractures. Int Orthop 28 (2004) 167-170
46. Kim J., Kim I., Cho T., et al. Bone regeneration using hyaluronic acid-based hydrogel with bone morphogenic protein-2 and human mesenchymal stem cells. Biomaterials 28 (2007) 1830-1837
47. Kobayashi T., Watanabe H., Yanagawa T., et al. Motility and growth of human bone-marrow mesenchymal stem cells during ex vivo expansion in autologous serum. J Bone Joint Surg Br 87 (2005) 1426-1433
48. Kocaoemer A., Kern S., Kluter H., and Bieback K. Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue. Stem Cells 25 (2007) 1270-1278
49. Koller M., Maher R., Manchel I., et al. Alternatives to animal sera for human bone marrow cell expansion: human serum and serum-free media. J Hematother 7 (1998) 413-423
50. Lennon D., and Caplan A. Isolation of human marrow-derived mesenchymal stem cells. Exp Hematol 34 (2006) 1604-1605
51. Lennon D., Osdoby P., Carrino D., et al. Isolation and characterization of chondrocytes and non-chondrocytes from high-density chick limb bud cell cultures. J Craniofac Genet Dev Biol 3 (1983) 235-251
52. Li Y., Chen J., Chen X., et al. Human marrow stromal cell therapy for stroke in rat: neurotrophins and functional recovery. Neurology 59 (2002) 514-523
53. Lieberman J., Daluiski A., Stevenson S., et al. The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats. J Bone Joint Surg Am 81 (1999) 905-917
54. Liechty K., MacKenzie T., Shaaban A., et al. Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep. Nat Med 6 (2000) 1282-1286
55. Lin H., Tarng Y., Chen Y., et al. Using human plasma supplemented medium to cultivate human bone marrow-derived mesenchymal stem cell and evaluation of its multiple-lineage potential. Transplant Proc 37 (2005) 4504-4505
56. Logeart-Avramoglou D., Anagnostou F., Bizios R., and Petite H. Engineering bone: challenges and obstacles. J Cell Mol Med 9 (2005) 72-84
57. Markusen J., Mason C., Hull D., et al. Behavior of adult human mesenchymal stem cells entrapped in alginate-GRGDY beads. Tissue Eng 12 (2006) 821-830
58. McAlinden M., and Wilson D. Comparison of cancellous bone-derived cell proliferation in autologous human and fetal bovine serum. Cell Transplant 9 (2000) 445-451
59. Meinel L., Hofmann S., Karageorgiou V., et al. Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffolds. Biotechnol Bioeng 88 (2004) 379-391
60. Meuleman N., Tondreau T., Delforge A., et al. Human marrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical alpha-MEM medium. Eur J Haematol 76 (2006) 309-316
61. Minguell J., Erices A., and Conget P. Mesenchymal stem cells. Exp Biol Med (Maywood) 226 (2001) 507-520
62. Muller I., Kordowich S., Holzwarth C., et al. Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy 8 (2006) 437-444
63. Muraglia A., Cancedda R., and Quarto R. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. J Cell Sci 113 (2000) 1161-1166
64. Murphy J., Fink D., Hunziker E., and Barry F. Stem cell therapy in a caprine model of osteoarthritis. Arthritis Rheum 48 (2003) 3464-3474
65. Muschler G., Boehm C., and Easley K. Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume. J Bone Joint Surg Am 79 (1997) 1699-1709
66. Muschler G., Nitto H., Matsukura Y., et al. Spine fusion using cell matrix composites enriched in bone marrow-derived cells. Clin Orthop Relat Res 407 (2003) 102-118
67. Na K., Kim S., Sun B., et al. Osteogenic differentiation of rabbit mesenchymal stem cells in thermo-reversible hydrogel constructs containing hydroxyapatite and bone morphogenic protein-2 (BMP-2). Biomaterials 28 (2007) 2631-2637
68. Odorico J., Kaufman D., and Thomson J. Multilineage differentiation from human embryonic stem cell lines. Stem Cells 19 (2001) 193-204
69. Oest M., Dupont K., Kong H., et al. Quantitative assessment of scaffold and growth factor-mediated repair of critically sized bone defects. J Orthop Res (2007) [Epub ahead of print].
70. Otto W., and Rao J. Tomorrow's skeleton staff: mesenchymal stem cells and the repair of bone and cartilage. Cell Prolif 37 (2004) 97-110
71. Palermo A., Labarge M., Doyonnas R., et al. Bone marrow contribution to skeletal muscle: a physiological response to stress. Dev Biol 279 (2005) 336-344
72. Peng L., Li H., Gu L., et al. Comparison of biological characteristics of marrow mesenchymal stem cells in hepatitis B patients and normal adults. World J Gastroenterol 13 (2007) 1743-1746
73. Peterson B., Zhang J., Iglesias R., et al. Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue. Tissue Eng 11 (2005) 120-129
74. Pittenger M., Mackay A., Beck S., et al. Multilineage potential of adult human mesenchymal stem cells. Science 284 (1999) 143-147
75. Pittenger M., and Martin B. Mesenchymal stem cells and their potential as cardiac therapeutics. Circ Res 95 (2004) 9-20
76. Ponticiello M., Schinagl R., Kadiyala S., and Barry F. Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. J Biomed Mater Res 52 (2000) 246-255
77. Pountos I, Georgouli T, Giannoudis PV. The effect of autologous serum obtained after fracture on the proliferation and osteogenic differentiation of Mesenchymal Stem Cells. British Trauma Society Annual meeting. 2006, Leeds, UK.
78. Pountos I., and Giannoudis P. Biology of mesenchymal stem cells. Injury 36 (2005) S8-S12
79. Pountos I., Jones E., Tzioupis C., et al. Growing bone and cartilage. The role of mesenchymal stem cells. J Bone Joint Surg Br 88 (2006) 421-426
80. Quarto R., Mastrogiacomo M., Cancedda R., et al. Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med 344 (2001) 385-386
81. Rahfoth B., Weisser J., Sternkopf F., et al. Transplantation of allograft chondrocytes embedded in agarose gel into cartilage defects of rabbits. Osteoarthritis Cartilage 6 (1998) 50-65
82. Robey P. Collagenase-treated trabecular bone fragments: a reproducible source of cells in the osteoblastic lineage. Calcif Tissue Int 56 (1995) S11-S12
83. Rubio D., Garcia-Castro J., Martin M., et al. Spontaneous human adult stem cell transformation. Cancer Res 65 (2005) 3035-3039
84. Saini S., and Wick T. Concentric cylinder bioreactor for production of tissue engineered cartilage: effect of seeding density and hydrodynamic loading on construct development. Biotechnol Prog 19 (2003) 510-521
85. Sakaguchi Y., Sekiya I., Yagishita K., et al. Suspended cells from trabecular bone by collagenase digestion become virtually identical to mesenchymal stem cells obtained from marrow aspirates. Blood 104 (2004) 2728-2735
86. Sakaguchi Y., Sekiya I., Yagishita K., and Muneta T. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum 52 (2005) 2521-2529
87. Schecroun N., and Delloye C. In vitro growth and osteoblastic differentiation of human bone marrow stromal cells supported by autologous plasma. Bone 35 (2004) 517-524
88. Seebach C., Henrich D., Tewksbury R., et al. Number and proliferative capacity of human mesenchymal stem cells are modulated positively in multiple trauma patients and negatively in atrophic nonunions. Calcif Tissue Int 80 (2007) 294-300
89. Selvaggi T., Walker R., and Fleisher T. Development of antibodies to fetal calf serum with arthus-like reactions in human immunodeficiency virus-infected patients given syngeneic lymphocyte infusions. Blood 89 (1997) 776-779
90. Shahdadfar A., Fronsdal K., Haug T., et al. In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells 23 (2005) 1357-1366
91. Shi E., Kazui T., Jiang X., et al. Intrathecal injection of bone marrow stromal cells attenuates neurologic injury after spinal cord ischemia. Ann Thorac Surg 81 (2006) 2227-2233
92. Sikavitsas V., Bancroft G., and Mikos A. Formation of three-dimensional cell/polymer constructs for bone tissue engineering in a spinner flask and a rotating wall vessel bioreactor. J Biomed Mater Res 62 (2002) 136-148
93. Simmons P., and Torok-Storb B. Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 78 (1991) 55-62
94. Simonsen J., Rosada C., Serakinci N., et al. Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol 20 (2002) 592-596
95. Siwach R., Sangwan S., Singh R., and Goel A. Role of percutaneous bone marrow grafting in delayed unions, non-unions and poor regenerates. Indian J Med Sci 55 (2001) 326-336
96. Sotiropoulou P., Perez S., Salagianni M., et al. Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells. Stem Cells 24 (2006) 462-471
97. Sotiropoulou P., Perez S., Salagianni M., et al. Cell culture medium composition and translational adult bone marrow-derived stem cell research. Stem Cells 24 (2006) 1409-1410
98. Spees J., Gregory C., Singh H., et al. Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy. Mol Ther 9 (2004) 747-756
99. Stenderup K., Justesen J., Clausen C., and Kassem M. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone 33 (2003) 919-926
100. Stute N., Holtz K., Bubenheim M., et al. Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use. Exp Hematol 32 (2004) 1212-1225
101. Thomas C., Kellam J., and Burg K. Comparative Study of Bone Cell Culture Methods for Tissue Engineering Applications. Journal of ASTM international 1 (2004) 1-17
102. Tsutsumi S., Shimazu A., Miyazaki K., et al. Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun 288 (2001) 413-419
103. Tuby H., Maltz L., and Oron U. Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med 39 (2007) 373-378
104. Tuli R., Seghatoleslami M., Tuli S., et al. A simple, highyield method for obtaining multipotential mesenchymal progenitor cells from trabecular bone. Mol Biotechnol 23 (2003) 37-49
105. Tuli R., Tuli S., Nandi S., et al. Characterization of multipotential mesenchymal progenitor cells derived from human trabecular bone. Stem Cells 21 (2003) 681-693
106. Tung J., Parks D., Moore W., et al. New approaches to fluorescence compensation and visualization of FACS data. Clin Immunol 110 (2004) 277-283
107. Tuschong L., Soenen S., Blaese R., et al. Immune response to fetal calf serum by two adenosine deaminase-deficient patients after T cell gene therapy. Hum Gene Ther 13 (2002) 1605-1610
108. Urdzikova L., Jendelova P., Glogarova K., et al. Transplantation of bone marrow stem cells as well as mobilization by granulocyte-colony stimulating factor promotes recovery after spinal cord injury in rats. J Neurotrauma 23 (2006) 1379-1391
109. Wakitani S., Goto T., Pineda S., et al. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg Am 76 (1994) 579-592
110. Walsh S., Jefferiss C., Stewart K., et al. Expression of the developmental markers STRO-1 and alkaline phosphatase in cultures of human marrow stromal cells: regulation by fibroblast growth factor (FGF)-2 and relationship to the expression of FGF receptors 1-4. Bone 27 (2000) 185-195
111. Wan C., He Q., McCaigue M., Marsh D., and Li G. Nonadherent cell population of human marrow culture is a complementary source of mesenchymal stem cells (MSCs). J Orthop Res 24 (2006) 21-28
112. Wang L., Li Y., Chen J., et al. Ischemic cerebral tissue and MCP-1 enhance rat bone marrow stromal cell migration in interface culture. Exp Hematol 30 (2002) 831-836
113. Wexler S., Donaldson C., Denning-Kendall P., et al. Adult bone marrow is a rich source of human mesenchymal 'stem' cells but umbilical cord and mobilized adult blood are not. Br J Haematol 121 (2003) 368-374
114. Wilkins R., Chimenti B., and Rifkin R. Percutaneous treatment of long bone nonunions: the use of autologous bone marrow and allograft bone matrix. Orthopedics 26 (2003) s549-s554
115. Wong G., Frantz K., and Lam C. Isolation and characterization of highly serum-dependent cells released early from collagenase digested calvaria. J Bone Miner Res 1 (1986) 417-424
116. Wu C., Chang J., and Zhai W. A novel hardystonite bioceramic: preparation and characteristics. Ceramics International 31 (2005) 27-31
117. Xi C., Haibo X., Chao W., et al. Bioreactor Expansion of Human Adult Bone Marrow-Derived Mesenchymal Stem Cells. Stem Cells 24 (2006) 2052-2059
118. Yamada Y., Boo J., Ozawa R., et al. Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold. J Craniomaxillofac Surg 31 (2003) 27-33
119. Yamamoto N., Isobe M., Negishi A., et al. Effects of autologous serum on osteoblastic differentiation in human bone marrow cells. J Med Dent Sci 50 (2003) 63-69
120. Yang S., Leong K., Du Z., and Chua C. The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Eng 7 (2001) 679-689
121. Yang X., Bhatnagar R., Li S., and Oreffo R. Biomimetic collagen scaffolds for human bone cell growth and differentiation. Tissue Eng 10 (2004) 1148-1159
122. Yokoyama S., Fukuda N., Li Y., et al. A strategy of retrograde injection of bone marrow mononuclear cells into the myocardium for the treatment of ischemic heart disease. J Mol Cell Cardiol 40 (2006) 24-34
123. Yu S., Tanabe T., Dezawa M., et al. Effects of bone marrow stromal cell injection in an experimental glaucoma model. Biochem Biophys Res Commun 344 (2006) 1071-1079
124. Zhao F., Grayson W., Ma T., et al. Effects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell construct development. Biomaterials 27 (2006) 1859-1867
125. Zhao F., and Ma T. Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: dynamic cell seeding and construct development. Biotechnol Bioeng 91 (2005) 482-493