Protein engineering in bioelectrocatalysis

Current Opinion in Biotechnology

Volumn 14, Issue 6, 2003, Pages 590-596

  Wong, Tuck Seng ^a   Schwaneberg, Ulrich ^a  

^a JACOBS UNIVERSITY BREMEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOFUEL; PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

BIOCATALYST; BIOSENSOR; CATALYSIS; ELECTROCHEMICAL ANALYSIS; ELECTRODE; ELECTRON TRANSPORT; ENZYME IMMOBILIZATION; ENZYME STABILITY; MUTATION; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN TRANSPORT; REVIEW; SYNTHESIS;

EID: 0345549552     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.copbio.2003.09.008     Document Type: Review

References (40)

1. Willner I., Katz E. Integration of layered redox proteins and conductive supports for bioelectronic application. Angew. Chem. Int. Ed. Engl. 39:2000;1180-1218 This comprehensive review presents various methods for the integration of redox proteins with electrode supports. Pictures are nicely drawn to illustrate the principles of various techniques and tables are provided to summarize experimental data and results.
2. Scheller F.W., Wollenberger U., Warsinke A., Lisdat F. Research and development in biosensors. Curr. Opin. Biotechnol. 12:2001;35-40.
3. Scheller F.W., Wollenberger U., Lei C., Jin W., Ge B., Lehmann C., Lisdat F., Fridman V. Bioelectrocatalysis by redox enzymes at modified electrodes. J. Biotechnol. 82:2002;411-424 This elegant paper provides a comprehensive summary on bioelectrocatalysis of glutathione peroxidases, cytochrome c and P450s at modified electrodes. The authors compare the bioelectrochemical behaviors when immobilized under various conditions and explain experimental results from a protein structure point of view.
4. Schuhmann W. Amperometric enzyme biosensors based on optimised electron-transfer pathways and non-manual immobilisation procedures. J. Biotechnol. 82:2002;425-441 This paper summarizes strategies for optimal electron transfer from electrodes to redox proteins or vice versa. Four main strategies with detailed examples are presented: direct electron transfer between redox proteins and electrodes modified with self-assembled monolayers; electron transfer cascades via redox hydrogels; anisotropic orientation of redox proteins at monolayer-modified electrodes; and electron transfer via conducting polymer films.
5. Marcus R.A., Sutin N. Electron transfers in chemistry and biology. Biochim. Biophys. Acta. 811:1985;265-322.
6. Urlacher V., Schmid R.D. Biotransformations using prokaryotic P450 monooxygenases. Curr. Opin. Biotechnol. 13:2002;557-564.
7. Niki K., Sprinkle J.R., Margoliash E. Intermolecular biological electron transfer: an electrochemical approach. Bioelectrochemistry. 55:2002;37-40.
8. Moosavi-Movahedi A.A., Chamani J., Ghourchian H., Shafiey H., Sorenson C.M., Sheibani N. Electrochemical evidence for the molten globule states of cytochrome c induced by N-alkyl sulfates at low concentrations. J. Protein Chem. 22:2003;23-30.
9. Bartlett P.N., Simon E., Toh C.S. Modified electrodes for NADH oxidation and dehydrogenase-based biosensors. Bioelectrochemistry. 56:2002;117-122.
10. Zhang S., Wright G., Yang Y. Materials and techniques for electrochemical biosensor design and construction. Biosens. Bioelectron. 15:2000;273-282.
11. Tiziani S., Sussich F., Cesaro A. The kinetics of periodate oxidation of carbohydrates 2. Polymeric substrates. Carbohydr Res. 338:2003;1083-1095.
12. Munro A.W., Leys D.G., McLean K.J., Marshall K.R., Ost T.W.B., Daff S., Miles C.S., Chapman S.K., Lysek D.A., Moser C.C.et al. P450 BM3: the very model of a modern flavocytochrome. Trends Biochem. Sci. 27:2002;250-257 This review summarizes structure-function relationships of a well-studied monooxygenase P450 BM-3 from Bacillus megaterium. The authors describe in detail the electron transfer within this monooxygenase and control mechanisms involved in avoiding futile cycling and hydrogen peroxide production. A short summary of prospective applications of P450 BM-3 is also provided.
13. Chaubey A., Malhotra B.D. Mediated biosensors. Biosens. Bioelectron. 17:2002;441-456.
14. +, to an electrode surface. The enzyme electrodes are remarkably stable and no leakage of the cofactor was observed during operation.
15. Kurtzman A.L., Govindarajan S., Vahle K., Jones J.T., Heinrichs V., Patten P.A. Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins. Curr. Opin. Biotechnol. 12:2001;361-370 This interesting review explains the principles of directed protein evolution and, in particular, advances in gene recombination methods. The pros and cons of each recombination method are considered. A complete list of recombinant protein therapeutics is included.
16. Katz E., Sheeney-Haj-Ichia L., Willner I. Magneto-switchable electrocatalytic and bioelectrocatalytic transformations. Chemistry. 8:2002;4138-4148.
17. Gelo-Pujic M., Kim H.H., Butlin N.G., Palmore G.T. Electrochemical studies of a truncated laccase produced in Pichia pastoris. Appl. Environ Microbiol. 65:1999;5515-5521.
18. Ferapontova E., Gorton L. Effect of pH on direct electron transfer in the system gold electrode-recombinant horseradish peroxidase. Bioelectrochemistry. 55:2002;83-87.
19. Ferapontova E., Schmengler K., Borchers T., Ruzgas T., Gorton L. Effect of cysteine mutations on direct electron transfer of horseradish peroxidase on gold. Biosens. Bioelectron. 17:2002;953-963.
20. Chen L.Q., Zhang X.E., Xie W.H., Zhou Y.F., Zhang Z.P., Cass A.E.G. Genetic modification of glucose oxidase for improving performance of an amperometric glucose biosensor. Biosens. Bioelectron. 17:2002;851-857.
21. Halliwell C.M., Simon E., Toh C.S., Cass A.E.G., Bartlett P.N. The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes. Bioelectrochemistry. 55:2002;21-23.
22. Davidson V.L. Probing mechanisms of catalysis and electron transfer by methylamine dehydrogenase by site-directed mutagenesis of αPhe55. Biochim. Biophys. Acta. 1647:2003;230-233.
23. Bao L., Sun D., Tachikawa H., Davidson V.L. Improved sensitivity of a histamine sensor using an engineered methylamine dehydrogenase. Anal. Chem. 74:2002;1144-1148.
24. Gilardi G., Meharenna Y.T., Tsotsou G.E., Sadeghi S.J., Fairhead M., Giannini S. Molecular lego: design of molecular assemblies of P450 enzymes for nanobiotechnology. Biosens. Bioelectron. 17:2002;133-145 An innovative approach to tailoring bioelectrochemical properties of redox proteins is presented by assembling catalytic and electron transfer 'domains' from closely related proteins. Examples of assembled proteins with enhanced bioelectrochemical performances are provided.
25. Wilson J.R., Caruana D.J., Gilardi G. Engineering redox functions in a nucleic acid binding protein. Chem. Commun. (Camb.). 7:2003;356-357.
26. Glieder A., Farinas E.T., Arnold F.H. Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase. Nat. Biotechnol. 20:2002;1135-1139 This outstanding paper describes how a fatty acid hydroxylating monooxygenase is converted into a powerful alkane hydroxylase and represents a remarkable example of directed evolution at its best. Alkane monooxygenases are of significant economical interest.
27. Cadwell R.C., Joyce G.F. Mutagenic PCR. PCR Methods Appl. 3:1994;S136-S140.
28. Bittker J.A., Le B.V., Liu D.R. Nucleic acid evolution and minimization by nonhomologous random recombination. Nat. Biotechnol. 20:2002;1024-1029.
29. Li Q.S., Schwaneberg U., Fischer M., Schmitt J., Pleiss J., Lutz-Wahl S., Schmid R.D. Rational evolution of a medium chain-specific cytochrome P450 BM-3 variant. Biochim. Biophys. Acta. 1545:2001;114-121.
30. Mano N., Mao F., Shin W., Chen T., Heller A. A miniature biofuel cell operating at 0.78 V. Chem. Commun. (Camb.). 21:2003;518-519.
31. Katz E., Willner I. A biofuel cell with electrochemically switchable and tunable power output. J. Am. Chem. Soc. 125:2003;6803-6813.
32. Mano N., Mao F., Heller A. Characteristics of a miniature compartment-less glucose-O-2 biofuel cell and its operation in a living plant. J. Am. Chem. Soc. 125:2003;6558-6594 This paper represents a significant leap in designing miniature biofuel cells for putative medical applications within the human body. Measuring the performance of the biofuel cell in a grape as living organism is creative and entertaining.
33. Kim H.H., Mano N., Zhang X.C., Heller A. A miniature membrane-less biofuel cell operating under physiological conditions at 0.5 V. J. Electrochem. Soc. 150:2003;209-213.
34. Tsujimura S., Kano K., Ikeda T. Glucose/O-2 biofuel cell operating at physiological conditions. Electrochemistry. 70:2002;940-942.
35. -1 with the natural cosubstrate oxygen.
36. Mao F., Mano N., Heller A. Long tethers binding redox centers to polymer backbones enhance electron transport in enzyme 'wiring' hydrogels. J. Am. Chem. Soc. 125:2003;4951-4957.
37. Munge B., Estavillo C., Schenkman J.B., Rusling J.F. Optimization of electrochemical and peroxide-driven oxidation of styrene with ultrathin polyion films containing cytochrome P450(cam) and myoglobin. Chembiochem. 4:2003;82-89.
38. Mayhew M.P., Reipa V., Holden M.J., Vilker V.L. Improving the cytochrome P450 enzyme system for electrode-driven biocatalysis of styrene epoxidation. Biotechnol. Prog. 16:2000;610-616.
39. Loechel C., Basran A., Basran J., Scrutton N.S., Hall E.A.H. Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode - Part 2: Rational design engineering of a 'wired' mutant. Analyst. 128:2003;889-898.
40. Mak K.K.W., Wollenberger U., Scheller F.W., Renneberg R. An amperometric bi-enzyme sensor for determination of formate using cofactor regeneration. Biosens. Bioelectron. 18:2003;1095-1100.