Sensitive assay for laboratory evolution of hydroxylases toward aromatic and heterocyclic compounds

Journal of Biomolecular Screening

Volumn 10, Issue 3, 2005, Pages 246-252

  Wong, T S ^a   Wu, N ^a   Roccatano, D ^a   Zacharias, M ^a   Schwaneberg, Ulrich ^a  

^a JACOBS UNIVERSITY BREMEN   (Germany)

Author keywords

Directed evolution; High throughput screening; Hydroxylation; Monooxygenase; P450

Indexed keywords

4 AMINOPHENAZONE; 4 CHROMANONE DERIVATIVE; AROMATIC COMPOUND; BENZOFURAN DERIVATIVE; CYTOCHROME P450; ETHER; HETEROCYCLIC COMPOUND; NAPHTHALENE; OCTANOL; OXYGENASE; PHENETOLE; PHENOL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TOLUENE; UNSPECIFIC MONOOXYGENASE;

ARTICLE; CATALYST; CELL LYSATE; CHEMICAL BOND; CHEMICAL STRUCTURE; COLORIMETRY; DIRECTED MOLECULAR EVOLUTION; ENZYME ASSAY; ENZYME SUBSTRATE; HYDROXYLATION; MUTAGENESIS; PRIORITY JOURNAL; ULTRAVIOLET SPECTROPHOTOMETRY;

BACTERIAL PROTEINS; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; HETEROCYCLIC COMPOUNDS; HYDROCARBONS, AROMATIC; MIXED FUNCTION OXYGENASES; MUTATION; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 18144363212     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057104273336     Document Type: Article

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