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As a reviewer has pointed out, flocculation of the QDs might be expected as the result of charge neutralization of the surface owing to the ion pairing with the cationic Cr(III) complexes. Such flocculation was indeed observed to occur very slowly upon standing for solutions at the highest quencher concentrations used. For this reason, photophysics measurements were only made with freshly prepared buffer solutions that did not indicate formation of insoluble particles
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As a reviewer has pointed out, flocculation of the QDs might be expected as the result of charge neutralization of the surface owing to the ion pairing with the cationic Cr(III) complexes. Such flocculation was indeed observed to occur very slowly upon standing for solutions at the highest quencher concentrations used. For this reason, photophysics measurements were only made with freshly prepared buffer solutions that did not indicate formation of insoluble particles.
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