Toxicity assays in nanodrops combining bioassay and morphometric endpoints

PLoS ONE

Volumn 2, Issue 1, 2007, Pages

  Lemaire, Frédéric ^a   Mandon, Céline A ^b   Reboud, Julien ^a   Papine, Alexandre ^c   Angulo, Jesus ^d   Pointu, Hervé ^a   Diaz Latoud, Chantal ^b   Lajaunie, Christian ^d   Chatelain, François ^a   Arrigo, André Patrick ^b   Schaack, Béatrice ^a  

^a DIF   (France)

^b UNIVERSITÉ LYON 1   (France)

^c IMSTAR SA   (France)

^d PSL RESEARCH UNIVERSITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARSENATE SODIUM; CADMIUM CHLORIDE; ENHANCED GREEN FLUORESCENT PROTEIN; EPIDERMAL GROWTH FACTOR; HEAT SHOCK PROTEIN; PARAQUAT; ARSENIC ACID DERIVATIVE; GREEN FLUORESCENT PROTEIN; HERBICIDE; NANOMATERIAL;

ARTICLE; BIOASSAY; CELL ASSAY; CELL ON CHIP DEVICE; CELL STRAIN HEPG2; CELL STRUCTURE; CELLULAR STRESS RESPONSE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DEVICE; GENE ACTIVATION; GENE EXPRESSION; GENETIC ENGINEERING; HUMAN; HUMAN CELL; IC 50; IMAGE ANALYSIS; IN VITRO STUDY; LIVER TOXICITY; MOLECULAR IMAGING; MORPHOMETRICS; PROMOTER REGION; TOXICITY TESTING; ANIMAL; CELL DEATH; CELL LINE; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; HIGH THROUGHPUT SCREENING; INSTRUMENTATION; LIVER CELL; METABOLISM; METHODOLOGY; MICROARRAY ANALYSIS; POLLUTANT;

ANIMALIA;

ANIMALS; ARSENATES; BIOLOGICAL ASSAY; CADMIUM CHLORIDE; CELL DEATH; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENVIRONMENTAL POLLUTANTS; GENE EXPRESSION REGULATION; GREEN FLUORESCENT PROTEINS; HEAT-SHOCK PROTEINS; HEPATOCYTES; HERBICIDES; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROARRAY ANALYSIS; NANOSTRUCTURES; PARAQUAT; PROMOTER REGIONS, GENETIC; TOXICITY TESTS;

EID: 38149123329     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0000163     Document Type: Article

References (42)

1. Balls M, Goldberg AM, Fentem JH, Broadhead CL, Burch RL, et al. (1995) The three Rs: the way forward: the report and recommendations of ECVAM Workshop 11. Altern Lab Anim 23: 838-866.
2. David CA, Middleton T, Montgomery D, Lim HB, Kati W, et al. (2002) Microarray compound screening (microARCS) to identify inhibitors of HIV integrase. J Biomol Screen 7: 259-266.
3. Schaack B Reboud J, Combe S, Fouqué B, Berger F, Boccard S, Filhol-Cochet O, Chatelain F (2005) A 'drop-chip' cell array for high throughput DNA and siRNA transfection combined with drug screening. NanoBiotechnology 1: 166-194.
4. Selinger DW, Wright MA, Church GM (2003) On the complete determination of biological systems. Trends Biotechnol 21: 251-254.
5. Baghdoyan S, Roupioz Y, Pitaval A, Castel D, Khomyakova E, et al. (2004) Quantitative analysis of highly parallel transfection in cell microarrays. Nucleic Acids Res 32: e77.
6. Yu H, Meyvantsson I, Shkel IA, Beebe DJ (2005) Diffusion dependent cell behavior in microenvironments. Lab Chip 5: 1089-1095.
7. Eldar A, Elowitz M (2005) Systems biology: deviations in mating. Nature 437: 631-632.
8. Wilson CJ, Si Y, Thompsons CM, Smellie A, Ashwell MA, et al. (2006) Identification of a small molecule that induces mitotic arrest using a simplified high-content screening assay and data analysis method. J Biomol Screen 11: 21-28.
9. Mandon CA, Diaz C, Arrigo AP, Blum LJ (2005) Chemical stress sensitive luminescent human cells: molecular biology approach using inducible Drosophila melanogaster hsp22 promoter. Biochem Biophys Res Commun 335: 536-544.
10. Mandon CA, Diaz-Latoud C, Arrigo AP, Blum LJ (2006) Dithiocarbamate fungicide thiram detection: comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction. J Biotechnol 124: 392-402.
11. Ait-Aissa S, Pandard P, Magaud H, Arrigo AP, Thybaud E, et al. (2003) Evaluation of an in vitro hsp70 induction test for toxicity assessment of complex mixtures: comparison with chemical analyses and ecotoxicity tests. Ecotoxicol Environ Saf 54: 92-104.
12. Vayssier M, Favatier F, Pinot F, Bachelet M, Polla BS (1998) Tobacco smoke induces coordinate activation of HSF and inhibition of NFkappaB in human monocytes: effects on TNFalpha release. Biochem Biophys Res Commun 252: 249-256.
13. Wong HR (1999) Heat shock proteins. Facts, thoughts, and dreams. A. De Maio. Shock 11:1-12, 1999. Shock 12: 323-325.
14. Knasmuller S, Mersch-Sundermann V, Kevekordes S, Darroudi F, Huber WW, et al. (2004) Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge. Toxicology 198: 315-328.
15. Fotakis G, Timbrell JA (2006) In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 160: 171-177.
16. Scheers EM, Ekwall B, Dierickx PJ (2001) In vitro long-term cytotoxicity testing of 27 MEIC chemicals on Hep G2 cells and comparison with acute human toxicity data. Toxicol In Vitro 15: 153-161.
17. Rossi MR, Somji S, Garrett SH, Sens MA, Nath J, et al. (2002) Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite. Environ Health Perspect 110: 1225-1232.
18. Elez D, Dundjerski J, Matic G (2001) Cadmium affects the redox state of rat liver glucocorticoid receptor. Cell Biol Toxicol 17: 169-177.
19. Vernhet L, Seite MP, Allain N, Guillouzo A, Fardel O (2001) Arsenic induces expression of the multidrug resistance-associated protein 2 (MRP2) gene in primary rat and human hepatocytes. J Pharmacol Exp Ther 298: 234-239.
20. Dragin N, Smani M, Arnaud-Dabernat S, Dubost C, Moranvillier I, et al. (2006) Acute oxidative stress is associated with cell proliferation in the mouse liver. FEBS Lett 580: 3845-3852.
21. Gottschalg E, Moore NE, Ryan AK, Travis LC, Waller RC, et al. (2006) Phenotypic anchoring of arsenic and cadmium toxicity in three hepatic-related cell systems reveals compound- and cell-specific selective up-regulation of stress protein expression: Implications for fingerprint profiling of cytotoxicity. Chem Biol Interact..
22. Pourahmad J, O'Brien PJ, Jokar F, Daraei B (2003) Carcinogenic metal induced sites of reactive oxygen species formation in hepatocytes. Toxicol In Vitro 17: 803-810.
23. Del Razo LM, Quintanilla-Vega B, Brambila-Colombres E, Calderon-Aranda ES, Manno M, et al. (2001) Stress proteins induced by arsenic. Toxicol Appl Pharmacol 177: 132-148.
24. Romero D, Gomez-Zapata M, Luna A, Garcia-Fernandez AJ (2003) Morphological characterisation of BGM (Buffalo Green Monkey) cell line exposed to low doses of cadmium chloride. Toxicol In Vitro 17: 293-299.
25. Thery M, Racine V, Pepin A, Piel M, Chen Y, et al. (2005) The extracellular matrix guides the orientation of the cell division axis. Nat Cell Biol 7: 947-953.
26. Aydin HH, Celik HA, Deveci R, Terzioglu E, Karacali S, et al. (2003) Characterization of the cellular response during apoptosis induction in cadmium-treated Hep G2 human hepatoma cells. Biol Trace Elem Res 95: 139-153.
27. Young IT (1977) Proof without prejudice: use of the Kolmogorov-Smirnov test for the analysis of histograms from flow systems and other sources. J Histochem Cytochem 25: 935-941.
28. Giuliano KA, Chen YT, Taylor DL (2004) High-content screening with siRNA optimizes a cell biological approach to drug discovery: defining the role of P53 activation in the cellular response to anticancer drugs. J Biomol Screen 9: 557-568.
29. Giuliano KA, Cheung WS, Curran DP, Day BW, Kassick AJ, et al. (2005) Systems cell biology knowledge created from high content screening. Assay Drug Dev Technol 3: 501-514.
30. Tencza SB, Sipe MA (2004) Detection and classification of threat agents via high-content assays of mammalian cells. J Appl Toxicol 24: 371-377.
31. Lee JH, Mitchell RJ, Kim BC, Cullen DC, Gu MB (2005) A cell array biosensor for environmental toxicity analysis. Biosens Bioelectron 21: 500-507.
32. Flaim CJ, Chien S, Bhatia SN (2005) An extracellular matrix microarray for probing cellular differentiation. Nat Methods 2: 119-125.
33. Lee MY, Park CB, Dordick JS, Clark DS (2005) Metabolizing enzyme toxicology assay chip (MetaChip) for high-throughput microscale toxicity analyses. Proc Natl Acad Sci U S A 102: 983-987.
34. Arrigo AP (2000) sHsp as novel regulators of programmed cell death and tumorigenicity. Pathol Biol (Paris) 48: 280-288.
35. Ait-Aissa S, Porcher J, Arrigo A, Lambre C (2000) Activation of the hsp70 promoter by environmental inorganic and organic chemicals: relationships with cytotoxicity and lipophilicity. Toxicology 145: 147-157.
36. Dudley RE, Svoboda DJ, Klaassen C (1982) Acute exposure to cadmium causes severe liver injury in rats. Toxicol Appl Pharmacol 65: 302-313.
37. Kondoh M, Tsukada M, Kuronaga M, Higashimoto M, Takiguchi M, et al. (2004) Induction of hepatic metallothionein synthesis by endoplasmic reticulum stress in mice. Toxicol Lett 148: 133-139.
38. Alexandrova AY, Kopnin PB, Vasiliev JM, Kopnin BP (2006) ROS up-regulation mediates Ras-induced changes of cell morphology and motility. Exp Cell Res.
39. Meyer F (2001) An Overview of Morphological Segmentation. International Journal of Pattern Recognition and Artificial Intelligence Vol. 15: 1089-1118.
40. Meyer F (2004) Levelings, Image Simplification Filters for Segmentation. Journal of Mathematical Imaging and Vision Vol. 20: 59-72.
41. Serra J (1999) Course on morphological operators. http://cmmensmpfr/~serra/acourshtm; Centre de Morphologie Mathématique, Ecole des Mines de Paris.
42. Angulo J, Serra J (2003) Automatic analysis of DNA microarray images using mathematical morphology. Bioinformatics 19: 553-562.