Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite

Environmental Health Perspectives

Volumn 110, Issue 12, 2002, Pages 1225-1232

  Rossi, Michael R ^a   Somji, Seema ^a,b   Garrett, Scott H ^a,b   Sens, Mary Ann ^a   Nath, Joginder ^a   Sens, Donald A ^a,b  

^a WEST VIRGINIA UNIVERSITY   (United States)

^b WEST VIRGINIA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Arsenic; Arsenite; Bladder; Bladder cancer; Bladder cell line; Heat shock proteins; Hsp; Immortalization; Stress response; Urothelium

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ARSENITE SODIUM; HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN 60; HEAT SHOCK PROTEIN 70; MESSENGER RNA;

ARTICLE; BLADDER; CELL DEATH; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION FUNCTION; CYTOTOXICITY; EXPOSURE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; LETHALITY; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STRESS; UROTHELIUM; WESTERN BLOTTING;

EUKARYOTA;

EID: 0036913861     PISSN: 00916765     EISSN: None     Source Type: Journal    
DOI: 10.1289/ehp.021101225     Document Type: Article

References (33)

1. ATSDR. 1997. Top 20 Hazardous Substances: ATSDR/EPA Priority List for 1997. Atlanta, GA:Agency for Toxic Substances and Disease Registry.
2. Aufricht C, Ardito T, Thulin G, Kashgarian M, Sieger NJ, Van Why SK. 1998. Heat-shock protein 25 induction and redistribution during actin reorganization after renal ischemia. Am J Physiol 274:F215-F222.
3. Bernstam L, Nriagu J. 2000. Molecular aspects of arsenic-stress. J Toxicol Environ Health B Crit Rev 3:293-322.
4. Bukau B, Horwich AL. 1998. The hsp 70 and hsp 60 chaperone machines. Cell 92:351-366.
5. Chiou HY, Hsueh Y, Liaw KF, Hong SF, Chiang MH, Pu YS, et al. 1995. Incidence of internal cancers and ingested inorganic arsenic: a 7-year follow-up study in Taiwan. Cancer Res. 55:1296-1300.
6. Ciocca DR, Oesterreich S, Chamness GC, McGuire WL, Fuqua SAW. 1993. Biological and clinical implications of heat shock protein 27000 (Hsp 27): a review. J Natl Cancer Inst 85:1558-1570.
7. Clark JI, Muchowski PJ. 2000. Small heat-shock proteins and their potential role in human disease. Curr Opin Struct Biol 10:52-59.
8. Craig EA, Weissman JS, Horwich AL. 1994. Heat shock proteins and molecular chaperones: mediators of protein conformation and turnover in the cell. Cell 78:365-372.
9. Denizot F, Lang R. 1986. Rapid colorimetic assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89:271-277.
10. 2+ induces metallothionein protein accumulation, but not metallothionein isoform 2 mRNA. Environ Health Perspect 106:587-595.
11. Georgopoulos C, Welch WJ. 1993. Role of the major heat shock proteins as molecular chaperones. Annu Rev Cell Biol 9:601-634.
12. Hopenhayn-Rich C, Biggs ML, Fuchs A, Bergoglio R, Tello EE, Nicolli H, et al. 1996. Bladder cancer mortality associated with arsenic in drinking water in Argentina. Epidemiology 7:117-124.
13. Jager J, Ostrosky-Wegman P. 1997. Arsenic: a paradoxical human carcinogen. Mutat Res 386:181-184.
14. Karagas MR, Tosteson TD, Blum J, Morris JS, Baron JA, Klaue B. 1998. Design of an epidemiologic study of drinking-water arsenic exposure and skin- and bladder-cancer risk in a U.S. population. Environ Health Perspect 106:1047-1050.
15. Kim D, Somji S, Garrett SH, Sens MA, Shukla S, Sens DA. 2001. Expression of hsp 27, hsp 50, hsc 70 and hsp 70 by immortalized human proximal tubule cells (HK-2) following exposure to heat shock, sodium arsenite or cadmium chloride. Toxicol Environ Health 63:475-493.
16. Kitchin KT. 2001. Recent advances in arsenic carcinogenesis: modes of action, animal model systems, and methylated arsenic metabolites. Toxicol Appl Pharmacol 172:249-261.
17. Macario AJL. 1995. Heat-shock proteins and molecular chaperones; implications for pathogenesis, diagnostics, and therapeutics. Int J Clin Lab Res 25:59-70.
18. Petzoldt JL, Leigh IM, Duffy PG, Masters JRW. 1994. Culture and characterization of human urothelium in vivo and in vitro. Urol Res 22:67-74.
19. Petzoldt JL, Leigh IM, Duffy PC, Sexton C, Masters JRW. 1995. Immortalization of human urothelial cells. Urol Res 23:377-380.
20. Rossi MR, Masters JRW, Park S, Todd JH, Garrett SH, Sens MA, et al. 2001. The immortalized UROtsa cell line as a cell culture model of human urothelium. Environ Health Perspect 109:801-808.
21. Schlesinger MJ. 1994. How the cell copes with stress and the function of heat shock proteins. Pediatr Res 36:1-6.
22. Schober A, Burger-Kentischer A, Müller E, Beck FX. 1998. Effect of ischemia on localization of heat shock protein 25 in kidney. Kidney Int 54:S174-S176.
23. Schober A, Müller E, Thurau K, Beck FX. 1997. The response of heat shock proteins 25 and 72 to ischaemia in different kidney zones. Pfluegers Arch Eur J Physiol 434:292-299.
24. Smith AH, Goycolea M, Haque R, Biggs ML. 1998. Marked increase in bladder and lung cancer mortality in a region of northern Chile due to arsenic in drinking water. Am J Epidemiol 147:660-669.
25. 2. Environ Health Perspect 107:545-552.
26. Somji S, Sens DA, Todd JH, Garrett SH, Nseyo UO, Sens MA. 1998. Expression of heat shock protein 27 in human bladder. Urol Pathol 9:1-15.
27. Somji S, Sens DA, Todd JH, Garrett SH, Nseyo UO, Sens MA. 1999b. Expression of heat shock protein 70 in the bladder from controls and patients with interstitial cystitis-like syndrome. Urol Pathol 10:9-21.
28. Somji S, Sens DA, Todd JH, Garrett SH, Nseyo UO, Sens MA. 1999c. The expression of heat shock protein 60 is reduced in the bladder of patients with interstitial cystitis. Urol Pathol 10:97-108.
29. 2. Environ Health Perspect 107:887-894.
30. 2. Toxicol Lett 115:127-136.
31. Steinmaus C, Moore L, Hopenhayn-Rich C, Biggs ML, Smith AH. 2000. Arsenic in drinking water and bladder cancer. Cancer Invest 18:174-182.
32. Tsuda T, Babazono A, Yamamoto E, Kurumatini N, Mino Y, Ogawa T, et al. 1995. Ingested arsenic and internal cancer: a historical cohort study followed for 33 years. Am J Epidemiol 141:198-209.
33. Valberg PA, Beck BD, Boardman PD, Chen JT. 1998. Liklihood ratio analysis of skin cancer prevalence associated with arsenic in drinking water in the US. Environ Geochem Health 20:61-66.