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Volumn 70, Issue 12, 2007, Pages 1003-1009

Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration

Author keywords

Dronpa; Fluorescence correlation spectroscopy; Fluorescence intensity distribution analysis; Fluorescence microscopy; Photoswitching; Reversibly switchable fluorescent proteins; Single molecule detection

Indexed keywords

FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; IRRADIATION; MOLECULES; SPECTROSCOPIC ANALYSIS;

EID: 37149025067     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20505     Document Type: Article
Times cited : (18)

References (50)
  • 1
    • 0036789916 scopus 로고    scopus 로고
    • An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
    • Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. 2002. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci USA 99:12651-12656.
    • (2002) Proc Natl Acad Sci USA , vol.99 , pp. 12651-12656
    • Ando, R.1    Hama, H.2    Yamamoto-Hino, M.3    Mizuno, H.4    Miyawaki, A.5
  • 2
    • 8844260411 scopus 로고    scopus 로고
    • Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting
    • Ando R, Mizuno H, Miyawaki A. 2004. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306:1370-1373.
    • (2004) Science , vol.306 , pp. 1370-1373
    • Ando, R.1    Mizuno, H.2    Miyawaki, A.3
  • 4
    • 33645731679 scopus 로고    scopus 로고
    • Fluorescence fluctuation spectroscopy in reduced detection volumes
    • Blom H, Kastrup L, Eggeling C. 2006. Fluorescence fluctuation spectroscopy in reduced detection volumes. Curr Pharm Biotechnol 7:51-66.
    • (2006) Curr Pharm Biotechnol , vol.7 , pp. 51-66
    • Blom, H.1    Kastrup, L.2    Eggeling, C.3
  • 5
    • 0344603641 scopus 로고    scopus 로고
    • The photon counting histogram in fluorescence fluctuation spectroscopy
    • Chen Y, Müller JD, So PTC, Gratton E. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys J 77:553-567.
    • (1999) Biophys J , vol.77 , pp. 553-567
    • Chen, Y.1    Müller, J.D.2    So, P.T.C.3    Gratton, E.4
  • 9
    • 33747199189 scopus 로고    scopus 로고
    • Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy
    • Dedecker P, Hotta J, Ando R, Miyawaki A, Engelborghs Y, Hofkens J. 2006. Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy. Biophys J 91:L45-L47.
    • (2006) Biophys J , vol.91
    • Dedecker, P.1    Hotta, J.2    Ando, R.3    Miyawaki, A.4    Engelborghs, Y.5    Hofkens, J.6
  • 10
    • 0030832226 scopus 로고    scopus 로고
    • On/off blinking and switching behaviour of single molecules of green fluorescent protein
    • Dickson RM, Cubitt AB, Tsien RY, Moerner WE. 1997. On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature 388:355-358.
    • (1997) Nature , vol.388 , pp. 355-358
    • Dickson, R.M.1    Cubitt, A.B.2    Tsien, R.Y.3    Moerner, W.E.4
  • 11
    • 27744441676 scopus 로고    scopus 로고
    • Characterization of the photoconversion on reaction of the fluorescent protein kaede on the single-molecule level
    • Dittrich PS, Schafer SP, Schwille P. 2005. Characterization of the photoconversion on reaction of the fluorescent protein kaede on the single-molecule level. Biophys J 89:3446-3455.
    • (2005) Biophys J , vol.89 , pp. 3446-3455
    • Dittrich, P.S.1    Schafer, S.P.2    Schwille, P.3
  • 12
    • 0032128026 scopus 로고    scopus 로고
    • Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis
    • Eggeling C, Widengren J, Rigler R, Seidel CAM. 1998. Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis. Anal Chem 70:2651-2659.
    • (1998) Anal Chem , vol.70 , pp. 2651-2659
    • Eggeling, C.1    Widengren, J.2    Rigler, R.3    Seidel, C.A.M.4
  • 13
    • 27744530935 scopus 로고    scopus 로고
    • Performance of fluorescence correlation spectroscopy for measuring diffusion and concentration
    • Enderlein J, Gregor I, Patra D, Dertinger T, Kaupp UP. 2005. Performance of fluorescence correlation spectroscopy for measuring diffusion and concentration. Chem Phys Chem 6:2324-2336.
    • (2005) Chem Phys Chem , vol.6 , pp. 2324-2336
    • Enderlein, J.1    Gregor, I.2    Patra, D.3    Dertinger, T.4    Kaupp, U.P.5
  • 15
    • 0242290934 scopus 로고    scopus 로고
    • Crystal gazing in optical microscopy
    • Fraser SE. 2003. Crystal gazing in optical microscopy. Nat Biotechnol 21:1272-1273.
    • (2003) Nat Biotechnol , vol.21 , pp. 1272-1273
    • Fraser, S.E.1
  • 18
    • 33745077089 scopus 로고    scopus 로고
    • Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: Mechanism responsible for the reversible photoswitching
    • Habuchi S, Dedecker P, Hotta J, Flors C, Ando R, Mizuno H, Miyawaki A, Hofkens J. 2006. Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: Mechanism responsible for the reversible photoswitching. Photochem Photobiol Sci 5:567-576.
    • (2006) Photochem Photobiol Sci , vol.5 , pp. 567-576
    • Habuchi, S.1    Dedecker, P.2    Hotta, J.3    Flors, C.4    Ando, R.5    Mizuno, H.6    Miyawaki, A.7    Hofkens, J.8
  • 19
    • 0242322565 scopus 로고    scopus 로고
    • Toward fluorescence nanoscopy
    • Hell SW. 2003. Toward fluorescence nanoscopy. Nat Biotechnol 21:1347-1355.
    • (2003) Nat Biotechnol , vol.21 , pp. 1347-1355
    • Hell, S.W.1
  • 20
    • 34249945258 scopus 로고    scopus 로고
    • Far-field optical nanoscopy
    • Hell SW. 2007. Far-field optical nanoscopy. Science 316:1153-1158.
    • (2007) Science , vol.316 , pp. 1153-1158
    • Hell, S.W.1
  • 21
    • 33845360042 scopus 로고    scopus 로고
    • Ultra-high resolution imaging by fluorescence photoactivation localization microscopy
    • Hess ST, Girirajan TPK, Mason MD. 2006. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91:4258-4272.
    • (2006) Biophys J , vol.91 , pp. 4258-4272
    • Hess, S.T.1    Girirajan, T.P.K.2    Mason, M.D.3
  • 22
    • 29144447893 scopus 로고    scopus 로고
    • Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
    • Hofmann M, Eggeling C, Jakobs S, Hell SW. 2005. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc Natl Acad Sci USA 102:17565-17569.
    • (2005) Proc Natl Acad Sci USA , vol.102 , pp. 17565-17569
    • Hofmann, M.1    Eggeling, C.2    Jakobs, S.3    Hell, S.W.4
  • 24
    • 0035248838 scopus 로고    scopus 로고
    • Two-color fluorescence correlation spectroscopy of one chromophore: Application to the E222Q mutant of the green fluorescent protein
    • Jung G, Bräuchle C, Zumbusch A. 2001. Two-color fluorescence correlation spectroscopy of one chromophore: Application to the E222Q mutant of the green fluorescent protein. J Chem Phys 114:3149-3156.
    • (2001) J Chem Phys , vol.114 , pp. 3149-3156
    • Jung, G.1    Bräuchle, C.2    Zumbusch, A.3
  • 25
    • 0033598837 scopus 로고    scopus 로고
    • Fluorescence-intensity distribution analysis and its application in biomolecular detection technology
    • Kask P, Palo K, Ullmann D, Gall K. 1999. Fluorescence-intensity distribution analysis and its application in biomolecular detection technology. Proc Natl Acad Sci USA 96:13756-13761.
    • (1999) Proc Natl Acad Sci USA , vol.96 , pp. 13756-13761
    • Kask, P.1    Palo, K.2    Ullmann, D.3    Gall, K.4
  • 26
    • 27144500688 scopus 로고    scopus 로고
    • Fluorescence fluctuation spectroscopy in subdiffraction focal volumes
    • Kastrup L, Blom H, Eggeling C, Hell SW. 2005. Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. Phys Rev Lett 94:178104.
    • (2005) Phys Rev Lett , vol.94 , pp. 178104
    • Kastrup, L.1    Blom, H.2    Eggeling, C.3    Hell, S.W.4
  • 27
    • 0343729418 scopus 로고    scopus 로고
    • Imaging and tracking of single GFP molecules in solution
    • Kubitscheck U, Kückmann O, Kues T, Peters R. 2000. Imaging and tracking of single GFP molecules in solution. Biophys J 78:2170-2179.
    • (2000) Biophys J , vol.78 , pp. 2170-2179
    • Kubitscheck, U.1    Kückmann, O.2    Kues, T.3    Peters, R.4
  • 29
    • 31144439832 scopus 로고    scopus 로고
    • Confining the sampling volume for fluorescence correlation spectroscopy using a sub-wavelength sized aperture
    • Leutenegger M, Gösch M, Perentes A, Hoffmann P, Martin OJF, Lasser T. 2006. Confining the sampling volume for fluorescence correlation spectroscopy using a sub-wavelength sized aperture. Opt Express 14:956-969.
    • (2006) Opt Express , vol.14 , pp. 956-969
    • Leutenegger, M.1    Gösch, M.2    Perentes, A.3    Hoffmann, P.4    Martin, O.J.F.5    Lasser, T.6
  • 31
    • 35949038838 scopus 로고
    • Thermodynamic fluctuations in a reacting system - measurement by fluorescence correlation spectroscopy
    • Magde D, Elson EL, Webb WW. 1972. Thermodynamic fluctuations in a reacting system - measurement by fluorescence correlation spectroscopy. Phys Rev Lett 29:705-708.
    • (1972) Phys Rev Lett , vol.29 , pp. 705-708
    • Magde, D.1    Elson, E.L.2    Webb, W.W.3
  • 32
    • 4244084400 scopus 로고
    • Optical detection and spectroscopy of single molecules in a solid
    • Moerner WE, Kador L. 1989. Optical detection and spectroscopy of single molecules in a solid. Phys Rev Lett 62:2535-2538.
    • (1989) Phys Rev Lett , vol.62 , pp. 2535-2538
    • Moerner, W.E.1    Kador, L.2
  • 34
    • 0040391480 scopus 로고
    • Single pentacene molecules detected by fluorescence excitation in a p-terphenyl crystal
    • Orrit M, Bernard J. 1990. Single pentacene molecules detected by fluorescence excitation in a p-terphenyl crystal. Phys Rev Lett 65:2716-2719.
    • (1990) Phys Rev Lett , vol.65 , pp. 2716-2719
    • Orrit, M.1    Bernard, J.2
  • 35
    • 0037072602 scopus 로고    scopus 로고
    • A photoactivatable GFP for selective photolabeling of proteins and cells
    • Patterson GH, Lippincott-Schwartz J. 2002. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297:1873-1877.
    • (2002) Science , vol.297 , pp. 1873-1877
    • Patterson, G.H.1    Lippincott-Schwartz, J.2
  • 36
    • 0021410769 scopus 로고
    • Optical stethoscopy: Image recording with resolution λ/20
    • Pohl DW, Denk W, Lanz M. 1984. Optical stethoscopy: Image recording with resolution λ/20. Appl Phys Lett 44:651-653.
    • (1984) Appl Phys Lett , vol.44 , pp. 651-653
    • Pohl, D.W.1    Denk, W.2    Lanz, M.3
  • 37
    • 11844263986 scopus 로고    scopus 로고
    • One- and two-photon photoactivation of a paGFP-fusion protein in live Drosophila embryos
    • Post JN, Lidke KA, Rieger B, Arndt-Jovin DJ. 2005. One- and two-photon photoactivation of a paGFP-fusion protein in live Drosophila embryos. FEBS Lett 579:325-330.
    • (2005) FEBS Lett , vol.579 , pp. 325-330
    • Post, J.N.1    Lidke, K.A.2    Rieger, B.3    Arndt-Jovin, D.J.4
  • 38
    • 1642588184 scopus 로고    scopus 로고
    • Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy
    • Ruckstuhl T, Seeger S. 2004. Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy. Opt Lett 29:569-571.
    • (2004) Opt Lett , vol.29 , pp. 569-571
    • Ruckstuhl, T.1    Seeger, S.2
  • 39
    • 33749026335 scopus 로고    scopus 로고
    • Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
    • Rust MJ, Bates M, Zhuang X. 2006. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3:793-796.
    • (2006) Nat Methods , vol.3 , pp. 793-796
    • Rust, M.J.1    Bates, M.2    Zhuang, X.3
  • 41
    • 23244453533 scopus 로고    scopus 로고
    • Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region
    • Schneider M, Barozzi S, Testa I, Faretta M, Diaspro A. 2005. Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region. Biophys J 89:1346-1352.
    • (2005) Biophys J , vol.89 , pp. 1346-1352
    • Schneider, M.1    Barozzi, S.2    Testa, I.3    Faretta, M.4    Diaspro, A.5
  • 44
    • 33846850271 scopus 로고    scopus 로고
    • 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
    • Stiel AC, Trowitzsch S, Weber G, Andresen M, Eggeling C, Hell SW, Jakobs S, Wahl MC. 2007. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants. Biochem J 402:35-42.
    • (2007) Biochem J , vol.402 , pp. 35-42
    • Stiel, A.C.1    Trowitzsch, S.2    Weber, G.3    Andresen, M.4    Eggeling, C.5    Hell, S.W.6    Jakobs, S.7    Wahl, M.C.8
  • 45
    • 0019366933 scopus 로고
    • Measuring surface dynamics of biomolecules by total internal reflection fluorescence with photobleaching recovery or correlation spectroscopy
    • Thompson NL, Burghardt TP, Axelrod D. 1981. Measuring surface dynamics of biomolecules by total internal reflection fluorescence with photobleaching recovery or correlation spectroscopy. Biophys J 33:435-454.
    • (1981) Biophys J , vol.33 , pp. 435-454
    • Thompson, N.L.1    Burghardt, T.P.2    Axelrod, D.3
  • 48
    • 0039025943 scopus 로고
    • Fluorescence correlation spectroscopy of triplet states in solution: A theoretical and experimental study
    • Widengren J, Mets Ü, Rigler R. 1995. Fluorescence correlation spectroscopy of triplet states in solution: A theoretical and experimental study. J Phys Chem 99:13368-13379.
    • (1995) J Phys Chem , vol.99 , pp. 13368-13379
    • Widengren, J.1    Mets, U.2    Rigler, R.3
  • 50
    • 0037832404 scopus 로고    scopus 로고
    • Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization
    • Yildiz A, Forkey JN, McKinney SA, Ha T, Goldman YE, Selvin PR. 2003. Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization. Science 300:2061-2065.
    • (2003) Science , vol.300 , pp. 2061-2065
    • Yildiz, A.1    Forkey, J.N.2    McKinney, S.A.3    Ha, T.4    Goldman, Y.E.5    Selvin, P.R.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.