Kindling fluorescent proteins for precise in vivo photolabeling

Nature Biotechnology

Volumn 21, Issue 2, 2003, Pages 191-194

  Chudakov, Dmitriy M ^a,b   Belousov, Vsevolod V ^b   Zaraisky, Andrey G ^a   Novoselov, Vladimir V ^a   Staroverov, Dmitry B ^a,b   Zorov, Dmitry B ^c   Lukyanov, Sergey ^a,b   Lukyanov, Konstantin A ^a,b  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b Evrogen JSC   (Russian Federation)

^c MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMARKERS; CELLS; FLUORESCENCE; PROTEINS; RNA;

PHOTOLABELING;

BIOTECHNOLOGY;

CELL PROTEIN; CHROMOPROTEIN; KINDLING FLUORESCENT PROTEIN; MESSENGER RNA; MUTANT PROTEIN; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL CELL; ARTICLE; BLEACHING; CELL LABELING; CELL MOTION; CELL STRUCTURE; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; IN VIVO STUDY; INTRACELLULAR TRANSPORT; IRRADIATION; MICROINJECTION; MITOCHONDRION; NONHUMAN; PHOTOACTIVATION; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; SEA ANEMONE; SPECTROSCOPY; VELOCITY; WAVEFORM; XENOPUS LAEVIS;

ANIMALS; CELL MOVEMENT; ESCHERICHIA COLI; FLUORESCENCE; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SEA ANEMONES; SPECTROMETRY, FLUORESCENCE; STAINING AND LABELING; XENOPUS LAEVIS;

ACTINIARIA; ANEMONE; ANEMONIA; ANEMONIA SULCATA; ANIMALIA; MAMMALIA; XENOPUS LAEVIS;

EID: 0347928662     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt778     Document Type: Article

References (25)

1. White, J. & Stelzer, E. Photobleaching GFP reveals protein dynamics inside live cells. Trends. Cell Biol. 9, 61-65 (1999).
2. Reits, E.A. & Neefjes, J.J. From fixed to FRAP: measuring protein mobility and activity in living cells. Nat. Cell Biol. 3, 145-147 (2001).
3. Lippincott-Schwartz, J., Snapp, E. & Kenworthy, A. Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2, 444-456 (2001).
4. Yokoe, H. & Meyer, T. Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nat. Biotechnol. 14, 1252-1256 (1996).
5. Elowitz, M.B., Surette, M.G., Wolf, P., Stock, J. & Leibler, S. Photoactivation turns green fluorescent protein red. Curr. Biol. 7, 809-812 (1997).
6. Marchant, J.S., Stutzmann, G.E., Leissring, M.A., LaFerla, F.M., & Parker, I. Multiphoton-evoked color change of DsRed as an optical highlighter for cellular and subcellular labeling. Nat. Biotechnol. 19, 645-649 (2001).
7. Dunn, G.A., Dobbie, I.M., Monypenny, J., Holt, M.R. & Zicha, D. Fluorescence localization after photobleaching (FLAP): a new method for studying protein dynamics in living cells. J. Microsc. 205, 109-112 (2002).
8. Lukyanov, K.A. et al. Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog. J. Biol. Chem. 275, 25879-25882 (2000).
9. Yang, F., Moss, L.G. & Phillips, G.N. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14, 1246-1251 (1996).
10. Ormö, M. et al. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395 (1996).
11. Wall, M.A., Socolich, M. & Ranganathan, R. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nat. Struct. Biol. 7, 1133-1138 (2000).
12. Yarbrough, D., Wachter, R.M., Kallio, K., Matz, M.V. & Remington, S.J. Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0 Å resolution. Proc. Natl. Acad. Sci. USA 98, 462-467 (2001).
13. Bulina, M., Chudakov, D., Mudrik, N. & Lukyanov, K. Interconversion of Anthozoa GFP-like fluorescent and non-fluorescent proteins by mutagenesis. BMC Biochem. 3, 7 (2002).
14. Yanushevich, Y.G. et al. A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins. FEBS Lett. 511, 11-14 (2002).
15. Keller, R. et al. Mechanisms of convergence and extension by cell intercalation. Phil. Trans. R. Soc. Lond. B Biol. Sci. 355, 897-922 (2000).
16. Condeelis, J.S., Wyckoff, J. & Segall, J.E. Imaging of cancer invasion and metastasis using green fluorescent protein. Eur. J. Cancer 36, 1671-1680 (2000).
17. Vajkoczy, P., Ullrich, A. & Menger, M.D. Intravital fluorescence videomicroscopy to study tumor angiogenesis and microcirculation. Neoplasia 2, 53-61 (2000).
18. Parish, C.R. Fluorescent dyes for lymphocyte migration and proliferation studies. Immunol. Cell Biol. 77, 499-508 (1999).
19. Campbell, R.E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 11, 7877-7882 (2002).
20. Matz, M.V. et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17, 969-973 (1999).
21. Patterson, G.H. & Lippincott-Schwartz, J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 13, 1873-1877 (2002).
22. Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H. & Miyawaki, A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 12651-12656 (2002).
23. Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K. & Pease, L.R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59 (1989).
24. Nieuwkoop, P.D. & Faber, J. Normal Table of Xenopus laevis (Daudin) (North-Holland, Amsterdam, 1967).
25. Patterson, G., Day, R. & N. Piston D. Fluorescent protein spectra. J. Cell Sci. 114, 837-838 (2001).