A survey on the cytogenetic status of the Croatian general population by use of the cytokinesis-block micronucleus assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 649, Issue 1-2, 2008, Pages 91-100

  Garaj Vrhovac, Verica ^a   Durinec, Martina ^a   Kopjar, Nevenka ^a   Oreščanin, Višnja ^b  

^a INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

^b RUDER BO KOVI INSTITUTE   (Croatia)

Author keywords

Age; Cytokinesis block proliferation index; Gender; Healthy volunteers; Micronucleus assay; Nuclear buds; Nucleoplasmic bridges; Smoking

Indexed keywords

BIOLOGICAL MARKER;

ADULT; ARTICLE; BIOLOGICAL MONITORING; CELL NUCLEUS INCLUSION BODY; CELL PROLIFERATION; CHROMOSOME ANALYSIS; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; CROATIA; CYTOKINESIS; FEMALE; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; MALE; MICRONUCLEUS; MICRONUCLEUS TEST; NORMAL HUMAN; POPULATION RESEARCH; PRIORITY JOURNAL; RANK SUM TEST; SEX DIFFERENCE; SMOKING HABIT; STATISTICAL SIGNIFICANCE; TOBACCO DEPENDENCE; VOLUNTEER;

ADULT; AGE FACTORS; CELL PROLIFERATION; CROATIA; CYTOKINESIS; DNA DAMAGE; FEMALE; HUMANS; LYMPHOCYTES; MALE; MICRONUCLEUS TESTS; MIDDLE AGED; SEX FACTORS; SMOKING;

EID: 36849022082     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2007.08.008     Document Type: Article

References (68)

1. Major J., Jakab M.G., and Tompa A. Genotoxicological monitoring of 175 subjects living in the green belts, inner town of near chemical estates in Greatest Budapest agglomeration. Mutat. Res. 412 (1998) 9-16
2. Maluf S.W. Monitoring DNA damage following radiation exposure using cytokinesis-block micronucleus method and alkaline single-cell gel electrophoresis. Clin. Chim. A 347 (2004) 15-24
3. Natarajan A.T., Boel J.J.W.A., Darroudi F., Van Diemen P.C.M., Dolout F., Hande M.P., and Ramalho A.T. Current cytogenetic methods for detecting exposure and effects of mutagens and carcinogens. Environ. Health Perspect. 104 (1996) 445-448
4. Albertini R.J., Anderson D., Douglas G.R., Hagmar L., Hemmiki K., Merlo F., Natarajan A.T., Norpa H., Shuker D.E.G., Tice R., Waters M.D., and Aitio A. ICPS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutat. Res. 463 (2000) 111-172
5. Anderson D., Jenkinson P.C., Dewdney R.S., Francis A.J., Godbert P., and Butterworth K.R. Chromosome aberrations, mitogen-induced blastogenesis and proliferative rate index in peripheral lymphocytes from 106 control individuals of the UK population. Mutat. Res. 204 (1988) 407-420
6. Everett R.D. Interactions between DNA viruses. ND10 and the DNA damage response. Cell. Microbiol. 8 (2006) 365-374
7. Wiesmuller L., Ford J.M., and Schiestl R.H. DNA damage, repair, and diseases. J. Biomed. Biotechnol. 2 (2002) 45-145
8. Andreassi M.G., Cioppa A., Bolto N., Joksic G., Manfredi S., Federici C., Ostojic M., Rubino P., and Picano E. Somatic DNA damage in interventional cardiologist: a case-control study. FASE J. 19 (2005) 998-999
9. Smolkova B., Dusinska M., Raslova K., McNeill G., Spustova V., Blazicek P., Horska A., and Collins A. Seasonal changes in markers of oxidative damage to lipids and DNA: correlation with seasonal variation in diet. Mutat. Res. 551 (2004) 135-144
10. Hagmar L., Bonassi S., Stromberg U., Mikoczy Z., Lando C., Hanstein I.L., Huici Montagud A., Knudsen L., Norpa H., Reuterwall C., Tinnerberg H., Brogger A., Forni A., Hogstedt B., Lambert B., Mitelman F., Norderson I., Salomaa S., and Skerfving S. Cancer predictive value of cytogenetic markers used in occupational health surrveilance programs: a report from an ongoing study by the European Study Group on Cytogenetic Biomakers and Health. Mutat. Res. 405 (1998) 171-178
11. Kirsch-Volders M., Sofuni T., Aardema M., Albertini S., Eastmond D., Fenech M., Ishidate M., Lorge E., Norppa H., Surralles J., von der Hude W., and Wakata A. Report from the in vitro micronucleus assay working group. Environ. Mol. Mutagen. 35 (2000) 167-172
12. Avlasevich S.L., Bryce S.M., Cairns S.E., and Dertinger S.D. In vitro micronucleus scoring by flow cytometry: differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability. Environ. Mol. Mutagen. 47 (2005) 56-66
13. Kirsch-Volders M., Sofuni T., Aardema M., Albertini S., Eastmond D., Fenech M., Ishidate M., Kirchner S., Lorge E., Morita T., Norppa H., Surralles J., Vanhauwaert A., and Wakata A. Report from the in vitro micronucleus assay working group. Mutat. Res. 540 (2003) 153-163
14. Fenech M., Holland N., Chang W.P., Zeiger E., and Bonassi S. The Human MicroNucleus Project-an international collaborative study on the use of micronucleus technique for measuring DNA damage in humans. Mutat. Res. 428 (1999) 271-283
15. Fenech M., and Morley A.A. Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low-dose X-irradiation. Mutat. Res. 161 (1986) 193-198
16. Huber R., Streng S., and Bauchinger M. The suitability of the human lymphocyte micronucleus assay system for biological dosimetry. Mutat. Res. 111 (1983) 185-193
17. Thierens H., Vral A., and de Ridder L. Biological dosimetry using the micronucleus assay for lymphocytes:interindividual differences in dose response. Health Phys. 61 (1991) 623-630
18. Countryman P.I., and Heddle J.A. The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutat. Res. 41 (1976) 321-332
19. Ramalho A., Sunjevaric I., and Natarajan A.T. Use of the frequencies of micronuclei as quantitative mediators of X-ray induced chromosomal aberrations in human peripheral blood lymphocytes: comparison of two methods. Mutat. Res. 207 (1988) 141-146
20. Lebana J.J., Uma N.P., and Aban M.S. Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionising radiation. Mutat. Res. 564 (2004) 83-88
21. Celik A., Cavas T., and Gozukara S.E. Cytogenetic biomonitoring in petrol station attendants: micronucleus test in exfoliated buccal cells. Mutagenesis 18 (2003) 417-421
22. Roma-Torres J., Teixeria J.P., Silva S., Laffou B., Cunha L.M., Mendez J., and Mangan O. Evaluation of genotoxicity in a group of workers from a petroleum refinery aromatics plants. Mutat. Res. 604 (2006) 19-27
23. Hesel H., Radon K., Pethran A., Maisch B., Grobmair S., Sautter I., and Fruhmann G. The genotoxic risk of hospital, pharmacy and medical personnel occupationally exposed cytostatic drugs-evaluation by the micronucleus assay. Mutat. Res. 497 (2001) 101-109
24. Orsiere T., Sari-Minodier I., Iarmacovai G., and Botta A. Genotoxic risk assessment of pathology and anatomy laboratory workers exposed to formaldehyde by use of personal air sampling and analysis of DNA damage in peripheral lymophocytes. Mutat. Res. 605 (2006) 30-41
25. Angelini S., Kumar R., Carbone F., Maffei F., Forti G.C., Violante F.S., Lodi V., Curti S., Hemminki K., and Patrizia Hrelia. Micronuclei in humans induced by exposure to low level of ionizing radiation: influence of polymorphisms in DNA repair genes. Mutat. Res. 570 (2005) 105-117
26. Migliore L., Parrini M., Sbrana I., Biagini C., Battaglia A., and Loprieno N. Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: the age effect. Mutat. Res. 256 (1991) 13-20
27. Goud K.I., Hasan Q., Balakrishna N., Prabhakar Rao K., and Ahuja Y.R. Genotoxicity evaluation of individuals working with photocopying machines. Mutat. Res. 563 (2004) 151-158
28. Thierens H., Vral A., and De Ridder L. A cytogenetic study of radiological workers: effect of age, smoking and radiation burden on the micronucleus frequency. Mutat. Res. 360 (1996) 75-82
29. Garaj-Vrhovac V.V. Micronucleus assay and lymphocyte mitotic activity in risk assessment of occupational exposure to microwave radiation. Chemosphere 39 (1999) 2301-2312
30. Garaj-Vrhovac V., Kopjar N., Besendorfer V., and Papeš D. Papeš Induction of micronuclei in human lymphocytes after occupational exposure to ultrasound. Chemosphere 38 (1999) 3541-3553
31. Garaj-Vrhovac V., and Kopjar N. Cytogenetic monitoring of cardiology unit hospital workers exposed to ultrasound. J. Appl. Toxicol. 20 (2000) 259-264
32. Köteles G.J., Bojtor I., Szirmal S., Berces J., and Otos M. Micronucleus frequency in cultured lymphocytes of an urban population. Mutat. Res. 319 (1993) 267-271
33. Bukvic N., Gentile M., Susca F., Fanelli M., Serio G., Buonadonna L., Capurso A., and Guanti G. Sex chromosome loss, micronuclei, sister chromatid exchange and aging: a study including 16 centenarians. Mutat. Res. 498 (2001) 159-167
34. Maffei F., Fimognari C., Cantelli-Forti G., and Hrelia P. Induction of aneuploidy in human lymphocytes by alcohol abuse. Mutat. Res. 379 (1997) S143
35. Cheng T.J., Christiani D.C., Xu X., Wain J.C., Wiencke J.K., and Kelsey K.T. Increased micronucleus frequency in lymphocytes from smokers with lung cancer. Mutat. Res. 349 (1996) 43-50
36. Holmén A., Karlsson A., Bratt I., and Högstedt B. Increased frequencies of micronuclei in T8 lymphocytes of smokers. Mutat. Res. 334 (1995) 205-208
37. Fenech M., and Morley A.A. The effect of donor age on spontaneous and induced micronuclei. Mutat. Res. 148 (1985) 99-105
38. Ganguly B.B. Cell division, chromosomal damage and micronucleus formation in peripheral lymphocytes of healthy donors: related to donor's age. Mutat. Res. 295 (1993) 135-148
39. Kirsch-Volders M., Sofuni T., Aardema M., Albertini S., Eastmond D., Fenech M., Ishidate M.J.R., Kirchner S., Lorge E., Morita T., Norppa H., Surralles J., Vanhauwaert A., and Wakata A. Corrigendum to report from the in vitro micronucleus assay working group. Mutat. Res. 564 (2004) 92-100
40. Kim J.S., Cho E.W., Chung H.W., and Kim I.G. Effects of tiron 4,5-dihydroxy-1,3-benzene disulfonic acid, on human promyelotic HL-60 leukemia cell differentiation and death. Toxicology 223 (2006) 36-45
41. Fenech M., Chang W.P., Kirsch-Volders M., Holland N., Bonassi S., and Zeiger E. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 534 (2003) 65-75
42. Zeljezic D., and Garaj-Vrhovac V. Chromosomal aberrations, micronuclei and nuclear buds induced in human lymphocytes by 2,4-dichlorophenoxyacetic acid pesticide formulation. Toxicology 200 (2004) 39-47
43. Kopjar N., and Garaj-Vrhovac V. Cytogenetic study of the Ag-NOR activity in micronuclei following the exposure to antineoplastic drugs. Toxic. Lett. (1998) 88
44. Fucic A., Garaj-Vrhovac V., Barkovic D., and Kubelka D. The sensitivity of the micronucleus assay for the detection of occupational exposure to vinyl chloride monomer. Mutat. Res. Lett. 325 (1994) 53-56
45. Zotti-Martelli L., Peccatori M., Maggini V., Ballardin M., and Barale R. Individual responsiveness to induction of micronuclei in human lymphocytes after exposure in vitro to 1800-MHz microwave radiation. Mutat. Res. 582 (2005) 42-52
46. Hoffmann H., and Speit G. Assessment of DNA damage in peripheral blood of heavy smokers with the comet assay and the micronucleus test. Mutat. Res. 581 (2005) 105-114
47. Fenech M. Important variables that influence base-line micronucleus frequency in cytokinesis-blocked lymphocytes-a biomarker for DNA damage in human populations. Mutat. Res. 404 (1998) 155-165
48. Kopjar N., Želježić D., and Garaj-Vrhovac V. Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test A. Biochim. Pol. 53 (2006) 321-336
49. Baeyens A., Vandersickel V., Thierens H., De Ridder L., and Vral A. Effects of estradiol and progesterone on the variability of the micronucleus assay. Mutat. Res. 578 (2005) 308-316
50. Fenech M., Neville S., and Rinaldi J. Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesis-blocked lymphocytes. Mutat. Res. 313 (1994) 203-207
51. Bolognesi C., Parrini M., Bonassi S., Ianello G., and Salanitto A. Cytogenetic analysis of a human population occupationally exposed to pesticides. Mutat. Res. 285 (1993) 239-249
52. Norman A., Bass D., and Roe D. Screening human populations for chromosome aberrations. Mutat. Res. 143 (1985) 155-160
53. Barale R., Chelotti L., Davini T., Del Ry S., Andreassi M.G., Ballardin M., Bulleri M., He J., Baldacci S., Di Pede F., Germignani F., and Landi S. Sister chromatid exchange and micronucleus frequency in human lymphocytes of 1650 subjects in an Italian population II. Contribution of sex, age and lifestyle. Environ. Mol. Mutagen. 31 (1998) 228-242
54. Bolognesi C., Lando C., Forni A., Landini E., Scarpato R., Migliore L., and Bonassi S. Chromosomal damage and ageing: effect on micronucleus frequency in peripheral blood lymphocytes. Age Ageing 28 (1999) 393-397
55. Bonassi S., Bolognesi C., Abbondandolo A., Barale R., Bigatti P., Camurri L., Dalpra L., De Ferrari M., Forni A., and Lando C. Influence of sex on cytogenetic end points: evidence from a large human sample and review of the literature. Cancer Epidemiol. Biomarkers Prev. 4 (1995) 671-679
56. Au W.W., Walker D.M., Ward J.B., Whorton E., Legator M.S., and Singh V. Factors contributing to the chromosome damage in lymphocytes of cigarette smokers. Mutat. Res. 260 (1991) 137-144
57. Wojda A., Zietkiewicz E., and Witt M. Effects of age and gender on micronucleus and chromosome nondisjunction frequencies and younger subjects. Mutagenesis 22 (2007) 195-200
58. Kašuba V., Rozgaj R., and Garaj-Vrhovac V. Micronucleus frequency in nurses occupationally exposed to cytostatic drugs. Toxic. Lett. 95 (1998) 88
59. Kašuba V., Šentija K., Garaj-Vrhovac V., and Fucic A. Chromosome aberrations in peripheral blood lymphocytes from control populations. Mutat. Res. Lett. 346 (1995) 187-193
60. Ishikawa H., Ishikawa T., Miyatsu Y., Kurihara K., Fukao A., and Yokoyama K. A polymorphism of the methionine synthase reductase gene increases chromosomal damage in peripheral lymphocytes in smokers. Mutat. Res. 599 (2006) 135-143
61. Donbak L., Rencuzogullari{dotless} E., Yavuz A., and Topaktas M. The genotoxic risk of underground coal miners from Turkey. Mutat. Res. 588 (2005) 82-87
62. Bonassi S., Neri M., Lando C., Ceppi M., Lin Y., Chang W.P., Holland N., Kirsch-Volders M., Zeiger E., and Fenech M. The HUMN collaborative group. Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project. Mutat. Res. 543 (2003) 155-166
63. Holz O., Scherer G., Brodtmeier S., Koops F., Warncke K., Krause T., Austen A., Angerer J., Tricker A.R., Adlkofer F., and Rüdiger H.W. Determination of low level exposure to volatile aromatic hydrocarbons and genotoxic effects in workers at a styrene plant. Occup. Environ. Med. 52 (1995) 420-428
64. Lemasters G..K., Livingston G.K., Lockey J.E., Olsen D.M., Shukia R., New G., Selevan S.G., and Ylin J.H. Genotoxic changes after low-level solvent and fuel exposure on aircraft maintenance personnel. Mutagenesis 12 (1997) 237-243
65. Lucero L., Pastor S., Suárez S., Durbán R., Gómez C., Parrón T., Creus A., and Marcos R. Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides: micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells. Mutat. Res. 464 (2000) 255-262
66. Mäki-Paakkanen J., Walles S., Osterman-Golkar S., and Norppa H. Single-strand breaks, chromosome aberrations, sister-chromatid exchanges, and micronuclei in blood lymphocytes of workers exposed to styrene during the production of reinforced plastics. Environ. Mol. Mutagen. 17 (1991) 27-31
67. Vaglenov A.K., and Karadjov A.G. Micronucleus frequencies in Bulgarian control population. Cent. Eur. J. Occup. Environ. Med. 3 (1997) 187-194
68. Minozzo R., Deimling L.I., Petrucci Gigante L., and Santos-Mello R. Micronucleus in peripheral blood lymphocytes of workers exposed to lead. Mutat. Res. 56 (2004) 53-60