Assessment of DNA damage in peripheral blood of heavy smokers with the comet assay and the micronucleus test

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 581, Issue 1-2, 2005, Pages 105-114

  Hoffmann, Heike ^a   Speit, Günter ^a  

^a UNIVERSITY HOSPITAL ULM   (Germany)

Author keywords

Aphidicolin; FPG protein; Gamma radiation; Human biomonitoring; Lymphocytes; Smoking

Indexed keywords

APC; APHIDICOLIN; BACTERIAL PROTEIN; DNA FORMAMIDOPYRIMIDINE GLYCOSYLASE;

ARTICLE; BLOOD SAMPLING; CELL ISOLATION; CIGARETTE SMOKING; COMET ASSAY; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DAMAGE; DNA DETERMINATION; DNA REPAIR; EXPERIMENTAL TEST; GAMMA RADIATION; GENE DUPLICATION; GENE FREQUENCY; GENETIC ANALYSIS; GENETIC VARIABILITY; GENOTOXICITY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LYMPHOCYTE; MALE; MICRONUCLEUS TEST; NORMAL HUMAN; PRIORITY JOURNAL; RADIATION EXPOSURE; SENSITIVITY ANALYSIS; STANDARD; STATISTICAL SIGNIFICANCE; TECHNIQUE;

BACTERIA (MICROORGANISMS);

EID: 13844271931     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2004.11.015     Document Type: Article

References (25)

1. F. Kassie, W. Parzefall, and S. Knasmuller Single cell gel electrophoresis assay: a new technique for human biomonitoring studies Mutat. Res. 463 2000 13 31
2. P. Moller, L.E. Knudsen, S. Loft, and H. Wallin The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors Cancer Epidemiol. Biomarkers Prev. 9 2000 1005 1015
3. G. Speit, and A. Hartmann The comet assay (single-cell gel test). A sensitive genotoxicity test for the detection of DNA damage and repair Methods Mol. Biol. 113 1999 203 212
4. G. Speit, K. Trenz, P. Schutz, A. Rothfuss, and O. Merk The influence of temperature during alkaline treatment and electrophoresis on results obtained with the comet assay Toxicol. Lett. 110 1999 73 78
5. R. Tice The single cell gel/comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells D.H. Phillips S. Venitt Environmental Mutagenesis 1995 BIOS Scientific Publishers Ltd. Oxford 315 339
6. G. Speit, T. Witton-Davies, W. Heepchantree, K. Trenz, and H. Hoffmann Investigations on the effect of cigarette smoking in the comet assay Mutat. Res. 542 2003 33 42
7. A.B. Nia, F.J. Van Schooten, P.A. Schilderman, T.M. De Kok, G.R. Haenen, M.H. Van Herwijnen, E. Van Agen, D. Pachen, and J.C. Kleinjans A multi-biomarker approach to study the effects of smoking on oxidative DNA damage and repair and antioxidative defense mechanisms Carcinogenesis 22 2001 395 401
8. A. Dhawan, N. Mathur, and P.K. Seth The effect of smoking and eating habits on DNA damage in Indian population as measured in the comet assay Mutat. Res. 474 2001 121 128
9. M. Dusinska, A. Ficek, A. Horska, K. Raslova, H. Petrovska, B. Vallova, M. Drlickova, S.G. Wood, A. Stupakova, J. Gasparovic, P. Bobek, A. Nagyova, Z. Kovacikova, P. Blazicek, U. Liegebel, and A.R. Collins Glutathione S-transferase polymorphisms influence the level of oxidative DNA damage and antioxidant protection in humans Mutat. Res. 482 2001 47 55
10. E. Diem, S. Ivancsits, and H.W. Rudiger Basal levels of DNA strand breaks in human leukocytes determined by comet assay J. Toxicol. Environ. Health A 65 2002 641 648
11. M.N. Mohankumar, S. Janani, B.K. Prabhu, P.R. Kumar, and R.K. Jeevanram DNA damage and integrity of UV-induced DNA repair in lymphocytes of smokers analysed by the comet assay Mutat. Res. 520 2002 179 187
12. I. Hininger, A. Chollat-Namy, S. Sauvaigo, M. Osman, H. Faure, J. Cadet, A. Favier, and A.M. Roussel Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers Mutat. Res. 558 2004 75 80
13. P. Vodicka, R. Kumar, R. Stetina, S. Sanyal, P. Soucek, V. Haufroid, M. Dusinska, M. Kuricova, M. Zamecnikova, L. Musak, J. Buchancova, H. Norppa, A. Hirvonen, L. Vodickova, A. Naccarati, Z. Matousu, and K. Hemminki Genetic polymorphisms in DNA repair genes and possible links with DNA repair rates, chromosomal aberrations and single-strand breaks in DNA Carcinogenesis 25 2004 757 763
14. W.W. Au, N. Cajas-Salazar, and S. Salama Factors contributing to discrepancies in population monitoring studies Mutat. Res. 400 1998 467 478
15. S. Bonassi, M. Neri, C. Lando, M. Ceppi, Y.P. Lin, W.P. Chang, N. Holland, M. Kirsch-Volders, E. Zeiger, and M. Fenech Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project Mutat. Res. 543 2003 155 166
16. G. Speit, P. Schutz, I. Bonzheim, K. Trenz, and H. Hoffmann Sensitivity of the FPG protein towards alkylation damage in the comet assay Toxicol. Lett. 146 2004 151 158
17. A. Rothfuss, P. Schutz, S. Bochum, T. Volm, E. Eberhardt, R. Kreienberg, W. Vogel, and G. Speit Induced micronucleus frequencies in peripheral lymphocytes as a screening test for carriers of a BRCA1 mutation in breast cancer families Cancer Res. 60 2000 390 394
18. M. Fenech The in vitro micronucleus technique Mutat. Res. 455 2000 81 95
19. G. Speit, P. Schutz, and H. Hoffmann Enhancement of genotoxic effects in the comet assay with human blood samples by aphidicolin Toxicol. Lett. 153 2004 303 310
20. M. De Boeck, N. Touil, G. De Visscher, P.A. Vande, and M. Kirsch-Volders Validation and implementation of an internal standard in comet assay analysis Mutat. Res. 469 2000 181 197
21. G. Speit, and A. Hartmann The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay) Mutagenesis 10 1995 555 559
22. R. Crebelli, P. Carta, C. Andreoli, G. Aru, G. Dobrowolny, S. Rossi, and A. Zijno Biomonitoring of primary aluminium industry workers: detection of micronuclei and repairable DNA lesions by alkaline SCGE Mutat. Res. 516 2002 63 70
23. W.W. Au, D.M. Walker, J.B. Ward Jr., E. Whorton, M.S. Legator, and V. Singh Factors contributing to chromosome damage in lymphocytes of cigarette smokers Mutat. Res. 260 1991 137 144
24. T. Albrecht, C.Z. Deng, S.Z. Abdel-Rahman, M. Fons, P. Cinciripini, and R.A. El Zein Differential mutagen sensitivity of peripheral blood lymphocytes from smokers and nonsmokers: effect of human cytomegalovirus infection Environ. Mol. Mutagen. 43 2004 169 178
25. W.W. Au Mutagen sensitivity assays in population studies Mutat. Res. 544 2003 273 277