Molecular characterization of the thi3 gene involved in thiamine biosynthesis in Zea mays: CDNA sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2- methylpyrimidine (phosphate) kinase and thiamine monophosphate synthase activities

Biochemical Journal

Volumn 408, Issue 2, 2007, Pages 149-159

  Rapala Kozik, Maria ^a   Olczak, Mariusz ^b   Ostrowska, Katarzyna ^a   Starosta, Agata ^a   Kozik, Andrzej ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

^b UNIVERSITY OF WROC AW   (Poland)

Author keywords

cDNA cloning; Enzyme kinetics; Protein overexpression; Site directed mutagenesis; Structural modelling; Thiamine phosphate diphosphorylase

Indexed keywords

PROTEIN OVEREXPRESSION; SITE-DIRECTED MUTAGENESIS; STRUCTURAL MODELING; THIAMINE PHOSPHATE DIPHOSPHORYLASE;

BIOSYNTHESIS; CHARACTERIZATION; CLONING; DNA SEQUENCES; ENZYME KINETICS; ESCHERICHIA COLI; MUTAGENESIS; PLANTS (BOTANY); THREE DIMENSIONAL;

GENES;

4 AMINO 5 HYDROXYMETHYL 2 METHYLPYRIMIDINE (PHOSPHATE) KINASE; ADENOSINE TRIPHOSPHATE; PROTEIN THI3; REGULATOR PROTEIN; THIAMINE; THIAMINE MONOPHOSPHATE SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE IDENTIFICATION; MAIZE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALKYL AND ARYL TRANSFERASES; AMINO ACID SEQUENCE; DNA, COMPLEMENTARY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PHOSPHOTRANSFERASES (PHOSPHATE GROUP ACCEPTOR); PLANT PROTEINS; PYRIMIDINES; RECOMBINANT PROTEINS; THIAMINE; ZEA MAYS;

BACILLUS SUBTILIS; ESCHERICHIA COLI; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM; ZEA MAYS;

EID: 36749024726     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070677     Document Type: Article

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