Improving the activity and stability of GL-7-ACA acylase CA130 by site-directed mutagenesis

Applied and Environmental Microbiology

Volumn 71, Issue 9, 2005, Pages 5290-5296

  Zhang, Wei ^a   Liu, Yuan ^a   Zheng, Huabao ^a   Yang, Sheng ^a   Jiang, Weihong ^a  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; CRYSTAL STRUCTURE; GENES; MUTAGENESIS;

AMINO ACID RESIDUES; CATALYTIC EFFICIENCY; ENZYMATIC ACTIVITY; GLUTARYL-7-AMINO CEPHALOSPORANIC ACID ACYLASE;

ENZYME KINETICS;

ADIPOYL 6 AMINOPENICILLINIC ACID; AMIDASE; AMINE; AMINO ACID; BACTERIAL ENZYME; GLUTARYL 7 AMINOCEPHALOSPORANIC ACID ACYLASE; PENICILLIN DERIVATIVE; UNCLASSIFIED DRUG;

ENZYME ACTIVITY;

ARTICLE; BACTERIAL STRAIN; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME IMMOBILIZATION; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENE EXPRESSION; GENE MUTATION; HALF LIFE TIME; HYDROLYSIS; MUTANT; NONHUMAN; PSEUDOMONAS; SITE DIRECTED MUTAGENESIS; WILD TYPE;

CEPHALOSPORINS; ENZYME STABILITY; ESCHERICHIA COLI; MUTAGENESIS, SITE-DIRECTED; PENICILLIN AMIDASE; PROTEIN ENGINEERING; PSEUDOMONAS; SUBSTRATE SPECIFICITY;

PSEUDOMONAS SP.;

EID: 25144504061     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.9.5290-5296.2005     Document Type: Article

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