Active Site Residues of Cephalosporin Acylase Are Critical Not Only for Enzymatic Catalysis but Also for Post-translational Modification

Journal of Biological Chemistry

Volumn 276, Issue 51, 2001, Pages 48376-48381

  Kim, Sanggu ^a   Kim, Youngsoo ^a  

^a Yeungnam University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ANTIBIOTICS; CATALYSIS; MUTAGENESIS;

PROTEOLYSIS;

ENZYMES;

7 AMINOCEPHALOSPORANIC ACID; AMIDASE; AMINO ACID; CEPHALOSPORIN DERIVATIVE; GLUTARIC ACID; HYDROLASE; SERINE; THREONINE; PENICILLIN AMIDASE;

AMINO ACID SEQUENCE; ARTICLE; BREVUNDIMONAS DIMINUTA; CATALYSIS; DEACYLATION; ENZYME ACTIVE SITE; MODEL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; ACYLATION; BINDING SITE; CHEMISTRY; GENETICS; HYDROLYSIS; METABOLISM; PROTEIN PROCESSING;

BREVUNDIMONAS DIMINUTA; PSEUDOMONAS;

ACYLATION; BINDING SITES; CATALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; PENICILLIN AMIDASE; PROTEIN PROCESSING, POST-TRANSLATIONAL; SUPPORT, NON-U.S. GOV'T;

EID: 0035930509     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M109603200     Document Type: Article

References (23)

1. Murzin, A. G., Brenner, S. E., Hubbard, T., and Chothia, C. (1995) J. Mol. Biol. 247, 536-540
2. Li, Y., Chen, J., Jiang, W., Mao, X., Zhao, G., and Wang, E. (1999) Eur. J. Biochem. 262, 713-719
3. Duggleby, H. J., Tolley, S. P., Hill, C. P., Dodson, E. J., Dodson, G., and Moody, P. C. (1995) Nature 373, 264-268
4. Brannigan, J. A., Dodson, G., Duggleby, H. J., Moody, P. C., Smith, J. L., Tomchick, D. R., and Murzin, A. G. (1995) Nature 378, 416-419
5. Lee, Y. S., Kim, H. W., and Park, S. S. (2000) J. Biol. Chem. 275, 39200-39206
6. Lee, Y. S., and Park, S. S. (1998) J. Bacteriol. 180, 4576-4582
7. Aramori, I., Fukagawa, M., Tsumura, M., Iwani, M., Ono, H., Ishitani, Y., Kojo, H., Kohsaka, M., Ueda, Y., and Imanaka, H. (1992) J. Ferment. Bioeng. 73, 185-192
8. Matsuda, A., Matsuyama, K., Yamamoto, K., Ichikawa, S., and Komatsu, K. (1987) J. Bacteriol. 169, 5815-5820
9. Tischer, W., Giesecke, U., Lang, G., Roder, A., and Wedekind, F. (1992) Ann. N. Y. Acad. Sci. 672, 502-509
10. Kim, Y., Yoon, K. H., Khang, Y., Turley, S., and Hol, W. J. G. (2000) Structure 8, 1059-1068
11. Ishii, Y., Saito, Y., Fujimura, T., Sasaki, H., Noguchi, Y., Yamada, H., Niwa, M., and Shimomura, K. (1995) Eur. J. Biochem. 230, 773-778
12. Saito, Y., Fujimura, T., Ishii, Y., Noguchi, Y., Miura, T., Niwa, M., and Shimomura, K. (1996) Appl. Environ. Microbiol. 62, 2919-2925
13. Kim, D.-W., Kang, S.-M., and Yoon, K.-H. (1999) J. Microbiol. 37, 200-205
14. Yoon, K.-H. (1999) GenBank™ accession number, AF251710
15. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
16. Lee, Y. S., Yang, H. C., and Park, S. S. (1996) J. Microbiol. Biotechnol. 6, 375-380
17. Kwon, T. H., Rhee, S., Lee, Y. S., and Park, S. S. (2000) J. Struct. Biol. 2000, 79-81
18. Jones, T. A., Zou, J. Y., Cowan, S. W., and Kjeldgaard, M. (1991) Acta Crystallogr Sect. A 47, 110-119
19. Wallace, A. C., Laskowski, R. A., and Thornton, J. M. (1995) Protein Eng. 8, 127-134
20. McRee, D. (1999) J. Struct. Biol. 125, 156-165
21. Merritt, E., and Bacon, D. J. (1997) Methods Enzymol. 277, 505-524
22. Nicholls, A., Sharp, K. A., and Honig, B. (1991) Proteins 11, 281-296
23. Kim, Y. and Hol, W. G. J. (2001) Chem. Biol., in press