Engineering of Pseudomonas aeruginosa lipase by directed evolution for enhanced amidase activity: Mechanistic implication for amide hydrolysis by serine hydrolases

Protein Engineering, Design and Selection

Volumn 20, Issue 7, 2007, Pages 339-346

  Nakagawa, Yuichi ^a   Hasegawa, Atsuko ^a   Hiratake, Jun ^a   Sakata, Kanzo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Amidase activity; Catalytic triad; Directed evolution; Leaving group protonation; Pseudomonas aeruginosa lipase

Indexed keywords

AMIDASE ACTIVITY; CATALYTIC TRIAD; DIRECTED EVOLUTION; LEAVING GROUP PROTONATION; PSEUDOMONAS AERUGINOSA LIPASE;

CATALYST ACTIVITY; ENZYME ACTIVITY; HYDROLYSIS; MUTAGENESIS;

LIPASES;

2 NAPHTHYL OLEATE; AMIDASE; AMIDE; BACTERIAL ENZYME; ESTERASE; N (2 NAPHTHYL)OLEAMIDE; OLEAMIDE; OLEIC ACID; SERINE DEHYDRATASE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; BINDING SITES; DIRECTED MOLECULAR EVOLUTION; KINETICS; LIPASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PSEUDOMONAS AERUGINOSA; SERINE ENDOPEPTIDASES;

PSEUDOMONAS AERUGINOSA;

EID: 34548289533     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm025     Document Type: Article

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