Engineering Δ9-16:0-Acyl Carrier Protein (ACP) Desaturase Specificity Based on Combinatorial Saturation Mutagenesis and Logical Redesign of the Castor Δ9-18:0-ACP Desaturase

Journal of Biological Chemistry

Volumn 276, Issue 24, 2001, Pages 21500-21505

  Whittle, Edward ^b   Shanklin, John ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ANTIBODIES; FATTY ACIDS; SUBSTRATES;

MUTANTS;

BIOCHEMISTRY;

ACYL CARRIER PROTEIN; ACYL COENZYME A DESATURASE; UNSATURATED FATTY ACID; ACYL (ACYL CARRIER PROTEIN)DESATURASE; ACYL-(ACYL-CARRIER-PROTEIN)DESATURASE; FATTY ACID; MIXED FUNCTION OXIDASE; PRIMER DNA; RECOMBINANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; AUXOTROPHY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; GENE DUPLICATION; GENETIC COMPLEMENTATION; HYDROPHOBICITY; MEMBRANE FLUIDITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN LOCALIZATION; SOLVATION; CHEMICAL STRUCTURE; CHEMISTRY; COMBINATORIAL CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETIC VARIABILITY; GENETICS; KINETICS; METABOLISM; PLANT; POINT MUTATION; PROTEIN SECONDARY STRUCTURE; RICINUS COMMUNIS; SITE DIRECTED MUTAGENESIS;

CASTOR;

AMINO ACID SUBSTITUTION; CASTOR BEAN; COMBINATORIAL CHEMISTRY TECHNIQUES; DNA PRIMERS; ESCHERICHIA COLI; FATTY ACIDS; GENETIC COMPLEMENTATION TEST; KINETICS; MIXED FUNCTION OXYGENASES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PLANTS, TOXIC; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; VARIATION (GENETICS);

EID: 0035877632     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M102129200     Document Type: Article

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