Expression of the Trichoderma reesei tyrosinase 2 in Pichia pastoris: Isotopic labeling and physicochemical characterization

Protein Expression and Purification

Volumn 55, Issue 1, 2007, Pages 147-158

  Westerholm Parvinen, Ann ^a   Selinheimo, Emilia ^a   Boer, Harry ^a   Kalkkinen, Nisse ^b   Mattinen, Maija ^a   Saloheimo, Markku ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Cu enzymes; Heterologous expression; NMR; Pichia pastoris; Tyrosinase

Indexed keywords

COPPER; MONOPHENOL MONOOXYGENASE; NITROGEN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; ENZYMOLOGY; GENETICS; GLYCOSYLATION; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; ISOTOPE LABELING; METABOLISM; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; PICHIA; PROTEIN FOLDING; PROTEIN PROCESSING; TRICHODERMA;

AMINO ACID SEQUENCE; COPPER; CULTURE MEDIA; GLYCOSYLATION; ISOTOPE LABELING; MOLECULAR SEQUENCE DATA; MONOPHENOL MONOOXYGENASE; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PICHIA; PROTEIN FOLDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; TRICHODERMA;

HYPOCREA JECORINA; PICHIA PASTORIS;

EID: 34547856995     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.04.014     Document Type: Article

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