Expression of the GM2-activator protein in the methylotrophic yeast Pichia pastoris, purification, isotopic labeling, and biophysical characterization

Protein Expression and Purification

Volumn 34, Issue 1, 2004, Pages 147-157

  Wendeler, Michaela ^a   Hoernschemeyer, Joerg ^a,c   John, Michael ^b   Werth, Norbert ^a   Schoeniger, Maike ^a   Lemm, Thorsten ^a,d   Hartmann, Rudolf ^a,e   Kessler, Horst ^b   Sandhoff, Konrad ^a  

^a UNIVERSITY OF BONN   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c F HOFFMANN LA ROCHE LTD   (Switzerland)

^d ROCHE INNOVATION CENTER MUNICH   (Germany)

^e Thetis IBN GmbH   (Germany)

Author keywords

GM2 activator; Isotopic labeling; Mass spectrometry; Pichia pastoris; Sphingolipids

Indexed keywords

EUKARYOTA; PICHIA; PICHIA PASTORIS; VECTORS;

EID: 1442264832     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.11.010     Document Type: Article

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