Development of a high-level expression system for deuterium-labeled human serum albumin

Japanese Journal of Physiology

Volumn 53, Issue 1, 2003, Pages 65-69

  Tomida, Mihoko ^a   Kimura, Masashi ^a   Kuwata, Kazuo ^a   Hayashi, Tomoya ^a   Okano, Yukio ^a   Era, Seiichi ^a  

^a GIFU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Human serum albumin; Partial D labeling; Pichia pastoris

Indexed keywords

DEUTERIUM; METHANOL; RECOMBINANT HUMAN SERUM ALBUMIN;

ARTICLE; CIRCULAR DICHROISM; CONCENTRATION (PARAMETERS); HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; NUCLEOTIDE SEQUENCE; PHYSICAL CHEMISTRY; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; YEAST;

DEUTERIUM; GENE EXPRESSION REGULATION; HUMANS; ISOTOPE LABELING; MAGNETIC RESONANCE IMAGING; PICHIA; RECOMBINANT PROTEINS; SERUM ALBUMIN; TRANSFORMATION, GENETIC;

EID: 0242432456     PISSN: 0021521X     EISSN: None     Source Type: Journal    
DOI: 10.2170/jjphysiol.53.65     Document Type: Article

References (6)

1. Peters T Jr: All about Albumin; Biochemistry, Genetics, and Medical Applications, Academic Press, New York, pp 79-132, 1996
2. Goodey AR: The production of heterologous plasma proteins. Trends Biotechnol 11: 430-433, 1993
3. Carter DC and Ho JX: Structure of serum albumin. Adv Protein Chem 45: 153-203, 1994
4. Flaux J, Bertelsen EB, Horwich AL, and Wüthrich K: NMR analysis of a 900K GroEL-GroES complex. Nature 418: 207-211, 2002
5. Kuwata K, Era S, and Sogami M: The kinetic studies on the intramolecular SH, S-S exchange reaction of bovine mercaptalbumin. Biochim Biophys Acta 1205: 317-324, 1994
6. Kim TR, Goto Y, Hirota N, Kuwata K, Denton H, Wu S-Y, Sawyer L, and Batt CA: High-level expression of bovine β-lactoglobulin in Pichia pastoris and characterization of its physical properties. Protein Eng 10: 1339-1345, 1997