A tightly regulated and reversibly inducible siRNA expression system for conditional RNAi-mediated gene silencing in mammalian cells

Journal of Gene Medicine

Volumn 9, Issue 7, 2007, Pages 620-634

  Wu, Ren Huang ^a   Cheng, Tsung Lin ^a   Lo, San Ren ^a   Hsu, Hui Chun ^a   Hung, Chuan Fu ^a   Teng, Chiao Fang ^a   Wu, Ming Ping ^a,b   Tsai, Wen Hui ^a,b   Chang, Wen Tsan ^a  

^a NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

^b CHI MEI MEDICAL CENTER   (Taiwan)

Author keywords

Conditional RNAi system; Human H1 promoter; Inducible siRNA expression system; IPTG; Lac repressor operator; p53

Indexed keywords

DNA DIRECTED RNA POLYMERASE III; ISOPROPYL THIOGALACTOSE; LACTOSE; LUCIFERASE; PROTEIN P53; RIBONUCLEASE P; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE SILENCING; GENE SWITCHING; GENETIC REGULATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; OPERATOR GENE; PRIORITY JOURNAL; REPRESSOR GENE; RNA INTERFERENCE;

ANIMALS; CELL LINE; CRICETINAE; GENE EXPRESSION; GENETIC VECTORS; HELA CELLS; HUMANS; ISOPROPYL THIOGALACTOSIDE; LUCIFERASES; OPERATOR REGIONS (GENETICS); PROMOTER REGIONS (GENETICS); REPRESSOR PROTEINS; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION; TUMOR SUPPRESSOR PROTEIN P53;

MAMMALIA;

EID: 34447534388     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.1048     Document Type: Article

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