Conditional gene silencing utilizing the lac repressor reveals a role of SHP-2 in cagA-positive Helicobacter pylori pathogenicity

Cancer Science

Volumn 95, Issue 5, 2004, Pages 442-447

  Higucchi, Megumi ^a   Tsutsumi, Ryouhei ^a   Higashi, Hideaki ^a   Hatakeyama, Masanori ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CAGA PROTEIN; GALACTOSE; LACTOSE; PROTEIN TYROSINE PHOSPHATASE; PROTEIN TYROSINE PHOSPHATASE SHP 2; PYRANOSIDE; REPRESSOR PROTEIN; RNA; RNA POLYMERASE II; THIOL DERIVATIVE;

ARTICLE; BACTERIAL VIRULENCE; CELL STRUCTURE; CELL TRANSFORMATION; CONTROLLED STUDY; EPITHELIUM CELL; GENE EXPRESSION; GENE SILENCING; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; MORPHOGENESIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; RNA INTERFERENCE; STOMACH EPITHELIUM;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC VECTORS; HELICOBACTER PYLORI; REPRESSOR PROTEINS; RNA INTERFERENCE; RNA POLYMERASE III;

EID: 3042545272     PISSN: 13479032     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1349-7006.2004.tb03229.x     Document Type: Article

References (31)

1. Hannon GJ. RNA interference. Nature 2002; 418: 244-51.
2. Paddison PJ, Hannon GJ. RNA interference: the new somatic cell genetics? Cancer Cell 2002; 2: 17-23.
3. Dykxhoorn DM, Novina CD, Sharp PA. Killing the messenger: short RNAs that silence gene expression. Nat Rev Mol Cell Biol 2003; 4: 457-67.
4. Tijsterman M, Ketting RF, Plasterk RH. The genetics of RNA silencing. Annu Rev Genet 2002; 36: 489-519.
5. Carthew RW. Gene silencing by double-stranded RNA. Curr Opin Cell Biol 2001; 13: 244-8.
6. Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 2002; 296: 550-3.
7. Yu JY, DeRuiter SL, Turner DL. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci USA 2002; 99: 6047-52.
8. Miyagishi M, Taira K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat Biotechnol 2002; 20: 497-500.
9. Blaser MJ, Perez-Perez GI, Kleanthous H, Cover TL, Peek RM, Chyou PH, Stemmermann GN, Nomura A. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res 1995; 55: 2111-5.
10. Personnet J, Friedman GD, Orentreich N, Vogelman H. Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection. Gut 1997; 40: 297-301.
11. Nomura N, Lee J, Stemmermann GN, Nomura RY, Perez-Perez GI, Blaser MJ. Helicobacter pylori CagA seropositivity and gastric carcinoma risk in a Japanese American population. J Infect Dis 2002; 186: 1138-44.
12. Covacci A, Censini S, Bugnoli M, Petracca R, Burroni D, Macchia G, Massone A, Papini E, Xiang Z, Figura N, Rappuoli R. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci USA 1993; 90: 5791-5.
13. Segal ED, Cha J, Lo J, Falkow S, Tompkins LS. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori. Proc Natl Acad Sci USA 1999; 96: 14559-64.
14. Asahi M, Azuma T, Ito S, Ito Y, Suto H, Nagai Y, Tsubokawa M, Tohyama Y, Maeda S, Omata M, Suzuki T, Sasakawa C. Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells. J Exp Med 2000; 191: 593-602.
15. Stein M, Rappuoli R, Covacci A. Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation. Proc Natl Acad Sci USA 2000; 97: 1263-8.
16. Odenbreit S, Puls J, Sedlmaier B, Gerland E, Fischer W, Haas R. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion. Science 2000; 287: 1497-500.
17. Backert S, Ziska E, Brinkmann V, Zimny-Arndt U, Fauconnier A, Jungblut PR, Naumann M, Meyer TF. Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus. Cell Microbiol 2000; 2: 155-64.
18. Higashi H, Tsutsumi R, Muto S, Sugiyama T, Azuma T, Asaka M, Hatakeyama M. SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein. Science 2002; 295: 683-6.
19. Higashi H, Tsutsumi R, Fujita A, Yamazaki S, Asaka M, Azuma T, Hatakeyama, M. Biological activity of the Helicobacter pylori virulence factor CagA is determined by variation in the tyrosine phosphorylation sites. Proc Natl Acad Sci USA 2002; 99: 14428-33.
20. Yamazaki S, Yamakawa A, Ito Y, Ohtani M, Higashi H, Hatakeyama M, Azuma T. The CagA protein of Helicobacter pylori is translocated into epithelial cells and binds to SHP-2 in human gastric mucosa. J Infect Dis 2003; 187: 334-7.
21. Hu MC, Davidson N. The inducible lac operator-repressor system is functional in mammalian cells. Cell 1987; 48: 555-66.
22. Brown M, Figge J, Hansen U, Wright C, Jeang KT, Khoury G, Livingston DM, Roberts TM. lac repressor can regulate expression from a hybrid SV40 early promoter containing a lac operator in animal. Cell 1987; 49: 603-12.
23. Hoshikawa Y, Amimoto K, Mizuguchi R, Hatakeyama M. Highly controlled heterologous gene expression through combined utilization of the tetracycline-repressible transactivator and the lac repressor. Anal Biochem 1998; 261: 211-8.
24. Saxton TM, Henkemeyer M, Gasca S, Shen R, Shalaby F, Feng G-S, Pawson T. Abnormal mesoderm patterning in mouse embryos mutant for the SH2 tyrosine phosphatase Shp-2. EMBO J 1994; 16: 2352-64.
25. Hannon GJ, Chubb A, Maroney P, Hannon G, Altman S, Nilsen TE. Multiple cis-acting elements are required for RNA polymerase III transcription of the gene encoding H1 RNA, the RNA component of human RNase P. J Biol Chem 1991; 266: 22796-9.
26. Neel BG. Structure and function of SH2-domain containing tyrosine phosphatases. Semin Cell Biol 1993; 6: 419-32.
27. Feng GS, Pawson T. Phosphotyrosine phosphatases with SH2 domains: regulators of signal transduction. Trend Genet 1994; 10: 54-8.
28. Czauderna F, Santel A, Hinz M, Fechtner M, Durieux B, Fisch G, Leenders F, Arnold W, Giese K, Klippel A, Kaufmann J. Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res 2003; 31: e127.
29. Wiznerowicz M, Trono D. Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. J Virol 2003; 77: 8957-61.
30. Matsukura S, Jones PA, Takai D. Establishment of conditional vectors for hairpin siRNA knockdowns. Nucleic Acids Res 2003; 31: e77.
31. van de Wetering M, Oving I, Muncan V, Pon Fong MT, Brantjes H, van Leenen D, Holstege FC, Brummelkamp TR, Agami R, Clevers H. Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. EMBO Rep 2003; 4: 609-15.