RNA interference in Trypanosoma brucei: a high-throughput engine for functional genomics in trypanosomatids?

Trends in Parasitology

Volumn 23, Issue 8, 2007, Pages 348-351

  Balaña Fouce, Rafael ^a   Reguera, Rosa M ^a  

^a UNIVERSITY OF LEÓN   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOME 1; EXPRESSION VECTOR; FUNCTIONAL GENOMICS; GENE FUNCTION; GENE SILENCING; HIGH THROUGHPUT SCREENING; LEISHMANIA MAJOR; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PHENOTYPE; RNA INTERFERENCE; SHORT SURVEY; TRANSPOSON; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

ANIMALS; GENE SILENCING; GENES, PROTOZOAN; GENOME, PROTOZOAN; OPEN READING FRAMES; RNA INTERFERENCE; RNA, PROTOZOAN; TRYPANOSOMA BRUCEI BRUCEI;

LEISHMANIA MAJOR; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI; TRYPANOSOMATIDAE;

EID: 34447503700     PISSN: 14714922     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pt.2007.06.008     Document Type: Short Survey

References (26)

1. Berriman M., et al. The genome of the African trypanosome Trypanosoma brucei. Science 309 (2005) 416-422
2. El-Sayed N.M., et al. The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science 309 (2005) 409-415
3. Ivens A.C., et al. The genome of the kinetoplastid parasite, Leishmania major. Science 309 (2005) 436-442
4. El-Sayed N.M., et al. Comparative genomics of trypanosomatid parasitic protozoa. Science 309 (2005) 404-409
5. Ngo H., et al. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 14687-14692
6. Clayton J. RNA interference: the silent treatment. Nature 431 (2004) 599-605
7. Volpe T.A., et al. Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi. Science 297 (2002) 1833-1837
8. Shi H., et al. An unusual Dicer-like1 protein fuels the RNA interference pathway in Trypanosoma brucei. RNA 12 (2006) 2063-2072
9. Djikeng A., et al. RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs. RNA 7 (2001) 1522-1530
10. Durand-Dubief M., and Bastin P. TbAGO1, an Argonaute protein required for RNA interference, is involved in mitosis and chromosome segregation in Trypanosoma brucei. BMC Biol. 1 (2003) 2
11. Shi H., et al. Function of the trypanosome Argonaute 1 protein in RNA interference requires the N-terminal RGG domain and arginine 735 in the Piwi domain. J. Biol. Chem. 279 (2004) 49889-49893
12. Shi H., et al. Argonaute protein in the early divergent eukaryote Trypanosoma brucei: control of small interfering RNA accumulation and retroposon transcript abundance. Mol. Cell. Biol. 24 (2004) 420-427
13. Shi H., et al. Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA 6 (2000) 1069-1076
14. Redmond S., et al. RNAit: an automated web-based tool for the selection of RNAi targets in Trypanosoma brucei. Mol. Biochem. Parasitol. 128 (2003) 115-118
15. Subramaniam C., et al. Chromosome-wide analysis of gene function by RNA interference in the African trypanosome. Eukaryot. Cell 5 (2006) 1539-1549
16. Decottignies A., et al. Schizosaccharomyces pombe essential genes: a pilot study. Genome Res. 13 (2003) 399-406
17. Ullu E., et al. RNA interference in protozoan parasites. Cell. Microbiol. 6 (2004) 509-519
18. Beverley S.M. Protozomics: trypanosomatid parasite genetics comes of age. Nat. Rev. Genet. 4 (2003) 11-19
19. DaRocha W.D., et al. Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi. Mol. Biochem. Parasitol. 133 (2004) 175-186
20. Catalanotto C., et al. Redundancy of the two dicer genes in transgene-induced posttranscriptional gene silencing in Neurospora crassa. Mol. Cell. Biol. 24 (2004) 2536-2545
21. Alsford S., et al. Multiplex analysis of RNA interference defects in Trypanosoma brucei. Mol. Biochem. Parasitol. 139 (2005) 129-132
22. Beverley S.M., et al. Putting the Leishmania genome to work: functional genomics by transposon trapping and expression profiling. Philos. Trans. R. Soc. Lond. B Biol. Sci. 357 (2002) 47-53
23. Kelly J.M. Trypanosomatid shuttle vectors: new tools for the functional dissection of parasite genomes. Parasitol. Today 11 (1995) 447-451
24. Wirtz E., et al. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 99 (1999) 89-101
25. Clayton C.E. Genetic manipulation of kinetoplastida. Parasitol. Today 15 (1999) 372-378
26. Motyka S.A., and Englund P.T. RNA interference for analysis of gene function in trypanosomatids. Curr. Opin. Microbiol. 7 (2004) 362-368