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For review articles on the use of combinatorial receptor libraries in supramolecular chemistry, see: a
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For review articles on the use of combinatorial receptor libraries in supramolecular chemistry, see: a) N. Srinivasan, J. D. Kilburn, Curr. Opin. Chem. Biol. 2004, 8, 305-310;
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For some examples of the use of combinatorial libraries in drug discovery, see: a
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For some examples of the use of combinatorial libraries in drug discovery, see: a) J. Buchardt, C. Bruun Schiodt, C. Krog-Jensen, J.-M. Delaisse, N. T. Foged, M. Meldal, J. Comb. Chem. 2000, 2, 624-638;
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In some cases it was found, for example, that the tags attached to the substrate for the library screening actually interfere with the on-bead binding. For one illustrative example, see
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In some cases it was found, for example, that the tags attached to the substrate for the library screening actually interfere with the on-bead binding. For one illustrative example, see: H. Wennemers, W. C. Still, Tetrahedron Lett. 1994, 35, 6413-6416.
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For reviews on the encoding and deconvolution of combinatorial libraries, see: a
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For reviews on the encoding and deconvolution of combinatorial libraries, see: a) R. L. Affleck, Curr. Opin. Chem. Biol. 2001, 5, 257-263;
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For a recent example of the limitations imposed by the need of a tagging strand ,see
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For a recent example of the limitations imposed by the need of a tagging strand ,see: J. Shepherd, T. Gale, K. B. Jensen, J. D. Kilburn, Chem. Eur. J. 2006, 12, 713-720.
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b) J. Hochlowski, D. Whittern, J. Pan, R. Swenson, Drugs Future 1999, 24, 539-554;
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In some cases, compounds on a solid support have been directly detected if special diagnostic or marker vibrational bands from individual characteristic functional groups that do not normally contribute to the matrix were present within the compound: a D. E. Pivonka, J. Comb. Chem. 2000, 2, 33-38;
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In some cases, compounds on a solid support have been directly detected if special diagnostic or marker vibrational bands from individual characteristic functional groups that do not normally contribute to the matrix were present within the compound: a) D. E. Pivonka, J. Comb. Chem. 2000, 2, 33-38;
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For a review on the use of optical spectroscopy in combinatorial chemistry, see
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For a review on the use of optical spectroscopy in combinatorial chemistry, see: H.-U. Gremlich, Biotechnol. Bioeng. 1998, 61, 179-187.
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So far only solid-phase-bound nanoparticles have been used for the detection of analytes in solution technique of on-bead injection, M. J. A. Canada, A. R. Medina, J. Frank, B. Lendl, Analyst 2002, 127, 1365-1369
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So far only solid-phase-bound nanoparticles have been used for the detection of analytes in solution (technique of on-bead injection): M. J. A. Canada, A. R. Medina, J. Frank, B. Lendl, Analyst 2002, 127, 1365-1369.
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Angew1
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This number is based on the following approximation: the laser focus is approximated by a cylindrical Vfocus, π r2 h) with a radius r (lateral) and a height h (axial, In our experiment, values of r, 2 μm and h, 20 μm are used, which represent an upper limit. The of a single bead (Vbead, 4/3 π r3) is calculated with a radius of r, 50 μm The absolute amount of compound 1 in the laser focus region is therefore given by Vfocus/V bead multiplied by the total absolute amount of receptor on an entire single bead (100 pm, which was approximately 50 femtomole. For the conventional Raman spectrum Figure 3, lower spectrum, a 20 mM solution was placed in a cubic cuvette of 5-mm height, and a laser of approximately 1-mm radius was used for probing an absolute number of about 300 nmole of 1
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7 smaller than in solution.
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A statistical analysis of 36 spectra showed that after baseline correction and normalization the mean relative standard deviation (RSD) was only 10% (for more details see the Supporting Information).
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A statistical analysis of 36 spectra showed that after baseline correction and normalization the mean relative standard deviation (RSD) was only 10% (for more details see the Supporting Information).
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As the experimental time in mapping approaches for Raman microspectroscopy critically depends on the number of spatially resolved measurements, very short acquisition times per individual Raman or SERS spectrum become crucial to an efficient implementation: S. Schlücker, M. D. Schaeberle, S. W. Huffman, I. W. Levin, Anal. Chem. 2003, 75, 4312-4318
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As the experimental time in mapping approaches for Raman microspectroscopy critically depends on the number of spatially resolved measurements, very short acquisition times per individual Raman or SERS spectrum become crucial to an efficient implementation: S. Schlücker, M. D. Schaeberle, S. W. Huffman, I. W. Levin, Anal. Chem. 2003, 75, 4312-4318.
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