Calfection: A novel gene transfer method for suspension cells

Biochimica et Biophysica Acta - Gene Structure and Expression

Volumn 1676, Issue 2, 2004, Pages 155-161

  Lindell, Jeanette ^a   Girard, Philippe ^a   Müller, Natalie ^a   Jordan, Martin ^a   Wurm, Florian ^a  

^a EPFL   (Switzerland)

Author keywords

293 cell; BHK; Calcium; CHO; Large scale; Mammalian cell; Suspension; Transient transfection

Indexed keywords

CALCIUM CHLORIDE; PLASMID DNA;

ARTICLE; BIOREACTOR; CONTROLLED STUDY; DILUTION; EVALUATION; GENE TARGETING; GENE TRANSFER; HUMAN; HUMAN CELL; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN EXPRESSION; ROOM TEMPERATURE; SUSPENSION CELL CULTURE;

MAMMALIA;

EID: 1642554446     PISSN: 01674781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbaexp.2003.11.016     Document Type: Article

References (21)

1. Wiethoff C.M., Middaugh C.R.J. Barriers to nonviral gene delivery. Pharm. Sci. 92:2003;203-217.
2. Hong K., Sherley J., Lauffenburger D.A. Methylation of episomal plasmids as a barrier to transient gene expression via a synthetic delivery vector. Biomol. Eng. 18:2001;185-192.
3. Chan C.K., Jans D.A. Using nuclear targeting signals to enhance non-viral gene transfer. Immunol. Cell Biol. 80:2002;119-130.
4. Lechardeur D., Lukacs G.L. Intracellular barriers to non-viral gene transfer. Curr. Gene Ther. 2:2002;183-194.
5. Thomas M., Klibanov A.M. Non-viral gene therapy: polycation-mediated DNA delivery. Appl. Microbiol. Biotechnol. 62:2003;27-34.
6. Kircheis R., Wightman L., Wagner E. Design and gene delivery activity of modified polyethylenimines. Adv. Drug Deliv. Rev. 53:2001;341-358.
7. Graham F.L., van der Eb A.J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 52:1973;456-467.
8. Jordan M., Schallhorn A., Wurm F.M. Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acids Res. 24:1996;596-601.
9. Seelos C. A critical parameter determining the aging of DNA-calcium-phosphate precipitates. Anal. Biochem. 245:1997;109-111.
10. Brown M.D., Schatzlein A.G., Uchegbu I.F. Gene delivery with synthetic (non-viral) carriers. Int. J. Pharm. 229:2001;1-21.
11. Girard P., Derouazi M., Baumgartner G., Bourgeois M., Jordan M., Jacko B., Wurm F.M. 100-liter transient transfection. Cytotechnology. 38:2002;15-21.
12. Miescher S., Zahn-Zabal M., DeJesus M., Moudry R., Fisch I., Vogel M., Kobr M., Imboden M.A., Kragten E., Bichler J., Mermod N., Stadler B.M., Amstutz H., Wurm F.M. CHO expression of a novel human recombinant IgG1 anti-RhD antibody isolated by phage display. Br. J. Haematol. 111:2000;157-166.
13. Girard P., Jordan M., Tsao M., Wurm F.M. Small-scale bioreactor system for process development and optimization. Biotechnol. Bioeng. 7:2001;117-119.
14. Kim H.H., Lee W.S., Yang J.M., Shin S. Basic peptide system for efficient delivery of foreign genes. Biochim. Biophys. Acta. 1640:2003;129-136.
15. Wurm F., Bernard A. Large-scale transient expression in mammalian cells for recombinant protein production. Curr. Opin. Biotechnol. 10:1999;156-159.
16. Lam A.M., Cullis P.R. Calcium enhances the transfection potency of plasmid DNA-cationic liposome complexes. Biochim. Biophys. Acta. 1463:2000;279-290.
17. Haberland A., Knaus T., Zaitsev S.V., Stahn R., Mistry A.R., Coutelle C., Haller H., Bottger M. Calcium ions as efficient cofactor of polycation-mediated gene transfer. Biochim. Biophys. Acta. 1445:1999;21-30.
18. Walters R., Welsh M. Mechanism by which calcium phosphate coprecipitation enhances adenovirus-mediated gene transfer. Gene Ther. 6:1999;1845-1850.
19. Knoll D.A., Fried M.G., Bloomfield V.A. Structure and Expression. Sarma R.H., Sarma M.H. DNA and its Drug Complexes. 1998;123-145 Adenine Press, New York.
20. Jordan M., Köhne C., Wurm F. Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: control of physicochemical parameters allows transfection in stirred media. Cytotechnology. 26:1998;39-47.
21. Kejnovsky E., Kypr J. Millimolar concentrations of zinc and other metal cations cause sedimentation of DNA. Nucleic Acids Res. 26:1998;5295-5299.