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CTF determination and correction in electron cryotomography
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This paper describes an algorithm to determine and correct the defocus gradient across tilted images, as will be necessary to reach higher resolution when macromolecular complexes are extracted and averaged from tomograms.
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Fernandez J.J., Li S., and Crowther R.A. CTF determination and correction in electron cryotomography. Ultramicroscopy 106 (2006) 587-596. This paper describes an algorithm to determine and correct the defocus gradient across tilted images, as will be necessary to reach higher resolution when macromolecular complexes are extracted and averaged from tomograms.
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The first report of tomographic analysis of cryosections. Although cutting artifacts and other technical challenges remain, the results are encouraging.
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Hsieh C.-E., Leith A., Mannella C.A., Frank J., and Marko M. Towards high-resolution three-dimensional imaging of native mammalian tissue: Electron tomography of frozen-hydrated rat liver sections. J Struct Biol 153 (2006) 1-13. The first report of tomographic analysis of cryosections. Although cutting artifacts and other technical challenges remain, the results are encouraging.
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50
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Focused ion beam milling of vitreous water: prospects for an alternative to cryo-ultramicrotomy of frozen-hydrated biological samples
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The authors describe an innovative method to prepare thin sections of frozen-hydrated materials through ion-beam milling. The idea looks promising, and could enable regions of larger prokaryotic cells (such as E. coli and B. subtilis) or biofilms (and of course eukaryotic cells!) to be reconstructed at the highest ECT resolutions possible.
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Marko M., Hsieh C., Moberlychan W., Mannalla C.A., and Frank J. Focused ion beam milling of vitreous water: prospects for an alternative to cryo-ultramicrotomy of frozen-hydrated biological samples. J Microsc 222 (2006) 42-47. The authors describe an innovative method to prepare thin sections of frozen-hydrated materials through ion-beam milling. The idea looks promising, and could enable regions of larger prokaryotic cells (such as E. coli and B. subtilis) or biofilms (and of course eukaryotic cells!) to be reconstructed at the highest ECT resolutions possible.
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Tong J., Arslan I., and Midgley P. A novel dual-axis iterative algorithm for electron tomography. J Struct Biol 153 (2006) 55-63
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53
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Tilt-series and electron microscope alignment for the correction of the non-perpendicularity of beam and tilt-axis
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Diez D.C., Seybert A., and Frangakis A.S. Tilt-series and electron microscope alignment for the correction of the non-perpendicularity of beam and tilt-axis. J Struct Biol 154 (2006) 195-205
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Castano Diez D., Müller H., and Frangakis A.S. Implementation and performance evaluation of reconstruction algorithms on graphics processors. J Struct Biol 157 (2007) 288-295
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Kerr R.A., Levine H., Sejnowski T.J., and Rappel W.-J. Division accuracy in a stochastic model of Min oscillations in Escherichia coli. Proc Natl Acad Sci USA 103 (2006) 347-352
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