Tilt-series and electron microscope alignment for the correction of the non-perpendicularity of beam and tilt-axis

Journal of Structural Biology

Volumn 154, Issue 2, 2006, Pages 195-205

  Díez, Daniel Castaño ^a   Seybert, Anja ^a   Frangakis, Achilleas S ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Alignment; Electron tomography; Image processing; Three dimensional marker model

Indexed keywords

ARTICLE; ARTIFACT REDUCTION; ELECTRON MICROSCOPE; ELECTRON MICROSCOPY; GEOMETRY; IMAGE PROCESSING; IMAGE QUALITY; IMAGE RECONSTRUCTION; MATHEMATICAL ANALYSIS; MODEL; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, ELECTRON; TOMOGRAPHY;

EID: 33646074519     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2005.12.009     Document Type: Article

References (14)

1. Baumeister W. Electron tomography: towards visualizing the molecular organization of the cytoplasm. Curr. Opin. Struct. Biol. 12 (2002) 679-684
2. Bohm J., Frangakis A.S., Hegerl R., Nickell S., Typke D., and Baumeister W. Toward detecting and identifying macromolecules in a cellular context: template matching applied to electron tomograms. Proc. Natl. Acad. Sci. USA 97 (2000) 14245-14250
3. Dennis J.E., and Schnabel R.B. Numerical Methods for Unsconstrained Optimization and Nonlinear Equations (1983), Englewood Cliffs, New Jersey
4. FEI., 2000. tecnai documentation. FEI company.
5. Hegerl R. The EM program package: a platform for image processing in biological electron microscopy. J. Struct. Biol. 116 (1996) 30-34
6. Hegerl, R., 1999. Conical reconstruction of single-axis tilt-series.
7. Hoppe W., and Typke D. Three-dimensional reconstruction of aperiodic objects in electron microscopy. In: Hoppe W., and Mason R. (Eds). Advances in Structure Research by Diffraction Methods. Unconventional Electron Microscopy for Molecular Structure Determination vol. 7 (1979), Vieweg, Braunschweig 137-190
8. Lanzavecchia S., Cantele F., Bellon P.L., Zampighi L., Kreman M., Wright E., and Zampighi G.A. Conical tomography of freeze-fracture replicas: a method for the study of integral membrane proteins inserted in phospholipid bilayers. J. Struct. Biol. 149 (2005) 87-98
9. Lawrence M.C. Least-squares method of alignment using markers. In: Frank J. (Ed). Electron Tomography: Three-dimensional Imaging with the Transmission Electron Microscope (1992), Plenum Press, New York and London 197-204
10. Marsh B.J., Mastronarde D.N., Buttle K.F., Howell K.E., and McIntosh J.R. Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography. Proc. Natl. Acad. Sci. USA 98 (2001) 2399-2406
11. Medalia O., Weber I., Frangakis A.S., Nicastro D., Gerisch G., and Baumeister W. Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298 (2002) 1209-1213
12. Penczek P., Marko M., Buttle K., and Frank J. Double-tilt electron tomography. Ultramicroscopy 60 (1995) 393-410
13. Typke, D., Hegerl, R., Nickell, S., Boehm, J. and Frangakis, A.S., 1999. Measurements of the perpendicularity if the beam-direction and tilt-axis in the CM300.
14. Zheng Q.S., Braunfeld M.B., Sedat J.W., and Agard D.A. An improved strategy for automated electron microscopic tomography. J. Struct. Biol. 147 (2004) 91-101