Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage

RNA

Volumn 13, Issue 4, 2007, Pages 521-535

  Niranjanakumari, Somashekarappa ^a   Day Storms, Jeremy J ^a,c   Ahmed, Mahiuddin ^a,d   Hsieh, John ^a   Zahler, Nathan H ^a   Venters, Ronald A ^b   Fierke, Carol A ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

^b DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Southeastern University   (United States)

^d STONY BROOK UNIVERSITY   (United States)

Author keywords

Cross linking; Hydroxyl radical cleavage; Ribozyme; RNase P; Structure; Transient kinetics

Indexed keywords

5 IODOACETAMIDO 1,10 O PHENANTHROLINE; AZIDOPHENACYL BROMIDE; BROMINE DERIVATIVE; CYSTEINE; HOLOENZYME; PHENANTHROLINE DERIVATIVE; RIBONUCLEASE P; TRANSFER RNA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BINDING AFFINITY; CROSS LINKING; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN RNA BINDING; PROTEIN VARIANT;

AMINO ACID SUBSTITUTION; BACILLUS SUBTILIS; BASE SEQUENCE; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CROSS-LINKING REAGENTS; CYSTEINE; ESCHERICHIA COLI; HOLOENZYMES; HYDROXYL RADICAL; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; RIBONUCLEASE P; RNA, BACTERIAL; RNA, CATALYTIC;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33947716757     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.308707     Document Type: Article

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