Analysis of ligand binding to a ribose biosensor using site-directed mutagenesis and fluorescence spectroscopy

Protein Science

Volumn 16, Issue 3, 2007, Pages 362-368

  Vercillo, Natalie C ^a   Herald, Kaitlin J ^a   Fox, John M ^a   Der, Bryan S ^a   Dattelbaum, Jonathan D ^a  

^a UNIVERSITY OF RICHMOND   (United States)

Author keywords

Biosensor; Fluorescence; Periplasmic binding proteins; Ribose binding protein

Indexed keywords

ALANINE; BINDING PROTEIN; FLUORESCENT DYE; RIBOSE; SCAFFOLD PROTEIN;

ARTICLE; BIOSENSOR; CONCENTRATION (PARAMETERS); CONJUGATE; COVALENT BOND; DISSOCIATION CONSTANT; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; MELTING POINT; MUTATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN STABILITY; RESIDUE ANALYSIS; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; STRUCTURAL HOMOLOGY; TEMPERATURE; WILD TYPE;

ALANINE; BINDING SITES; BIOSENSING TECHNIQUES; CIRCULAR DICHROISM; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; LIGANDS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PERIPLASMIC BINDING PROTEINS; PROTEIN BINDING; RIBOSE; SPECTROMETRY, FLUORESCENCE;

ESCHERICHIA COLI;

EID: 33847282451     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.062595707     Document Type: Article

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