Rationally designed fluorescently labeled sulfate-binding protein mutants: Evaluation in the development of a sensing system for sulfate

Biotechnology and Bioengineering

Volumn 78, Issue 5, 2002, Pages 517-526

  Shrestha, Suresh ^a   Salins, Lyndon L E ^a   Ensor, C Mark ^a   Daunert, Sylvia ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Conformational change; Escherichia coli; Fluorescence; Sensing system; Sulfate binding protein (SBP)

Indexed keywords

CONFORMATIONS; CRYSTAL STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE; NEGATIVE IONS; PROBES; PROTEINS; X RAY ANALYSIS;

FLUORESCENT PROBES;

MUTAGENESIS;

5 [[[(2 IODOACETYL)AMINO]ETHYL]AMINO]NAPHTHALENE 1 SULFONIC ACID; ACRYLODAN; ANION; BINDING PROTEIN; CYSTEINE; FLUORESCENT DYE; MUTANT PROTEIN; N [2 (1 MALEIMIDYL)ETHYL] 7 (DIETHYLAMINO)COUMARIN 3 CARBOXAMIDE; SERINE; SULFATE; SULFATE BINDING PROTEIN; THIOL GROUP; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; DIAGNOSTIC AGENT; ESCHERICHIA COLI PROTEIN; PERIPLASMIC BINDING PROTEIN; SBP PROTEIN, E COLI; SULFATE BINDING PROTEIN, BACTERIA; SULFATE-BINDING PROTEIN, BACTERIA;

ARTICLE; BINDING SITE; BIOSENSOR; CALIBRATION; CHEMICAL LABELING; CONCENTRATION RESPONSE; CONFORMATIONAL TRANSITION; CONJUGATION; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; LIGAND BINDING; MOLECULAR RECOGNITION; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY; BIOSYNTHESIS; CELL CULTURE; CHEMICAL STRUCTURE; CHEMISTRY; EVALUATION; GENETICS; METABOLISM; METHODOLOGY; NASA DISCIPLINE ENVIRONMENTAL HEALTH; NON-NASA CENTER; PROTEIN CONFORMATION; SENSITIVITY AND SPECIFICITY; SPECTROFLUOROMETRY;

BACTERIAL PROTEINS; CELLS, CULTURED; ESCHERICHIA COLI; FLUORESCENT DYES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PERIPLASMIC BINDING PROTEINS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; SULFATES; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; ESCHERICHIA COLI PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

NASA DISCIPLINE ENVIRONMENTAL HEALTH; NON-NASA CENTER;

EID: 0037023932     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10221     Document Type: Article

References (48)

1. Bacterial periplasmic transport system: Structure mechanism, and evolution
2. Regional and global distributions and lifetimes of sulfate aerosol from Mexico City and southeast China
3. Interaction between soluble P-selectin and soluble P-selectin glycoprotein ligand. 1. Equilibrium binding analysis
4. Fluorescence and dynamics in protein
5. Evidence for a sulfate-binding site external to the cell membrane of Salmonella typhimurium
6. Osmotic-shock-sensitive transport systems
7. Engineering the maltose binding protein for reagentless fluorescence sensing
8. Hydrogen sulfide
9. Intestinal effects of sulfate in drinking water on normal human subjects
10. Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase
11. Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate
12. Sulfate-binding protein dislikes protonated oxyacids, a molecular explanation
13. Engineered interdomain disulfide in the periplasmic receptor for sulfate transport reduces flexibility
14. Interdomain salt bridges modulate ligand-induced domain motion of the sulfate receptor protein for active transport
15. Sulfate-selective PVC membrane electrodes based on a derivative of imidazole as a neutral carrier
16. Estimation of the sulfate content of hydrothermal vent bacterial polysaccharides by Fourier transform infrared spectroscopy
17. A dynamical investigation of acrylodan-labeled mutant phosphate-binding protein
18. Determination of sulfate ion by potentiometric back-titration using sodium tetrakis (4-fluorophenyl) borate as a titrant and a titrant-sensitive electrode
19. Ion chromatography method and validation for the determination of sulfate and sulfamate ions in topiramate drug substance and finished product
20. Hydrogen sulfide poisoning: Clarification of some controversial issues
21. Determination of sulfur oxyanions by ion chromatography on a silica ODS column with terapropylammonium salt as an ion-pairing reagent
22. Sulphate determination after its reduction to hydrogen sulphide and volatile separation by molecular absorption spectrometry
23. Determination of sulfate in brackish waters by laser Raman spectroscopy
24. A microbial method using whole cells of Thiobacillus thiooxidans for measuring sulphate in waters
25. Embryonic mortality and abnormalities of aquatic birds: Apparent impacts of selenium from irrigation drain water
26. Genetics of sulfate transport by Salmonella typhimurium
27. Sulphate sequestered in the sulphate-binding protein of Salmonella typhimurium is bound solely by hydrogen bonds
28. The 2 Å resolution structure of the sulfate-binding protein involved in active transport in Salmonella typhimurium
29. Alternative indirect method for sulfate determination in natural samples
30. Atomic basis of the exquisite specificity of phosphate and sulfate transport receptors
31. Atomic structure and specificity of bacterial periplasmic receptors for active transport and chemotaxis: Variation of common themes
32. Stabilization of charges on isolated ionic groups sequestered in proteins by polarized peptide units
33. Periplasmic binding protein structure and function refined x-ray structures of the leucine/isoleucine/valine-binding protein and its complex with leucine
34. Optical sensing systems based on biomolecular recognition of recombinant proteins
35. A novel reagentless sensing sysem for measuring glucose based on the galactose/glucose binding protein
36. Sulfate and thiosulfate transport in Escherichia coli K-12: Nucleotide sequence and expression of the cysTWAM gene cluster
37. Sulfate and thiosulfate transport in Escherichia coli K-12: Evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins
38. Determination of sulphate in natural waters and industrial effluents by sequential injection analysis
39. Determination of picomolar concentrations of metal ions using fluorescence anisotropy: Biosensing with a "reagentless" enzyme transducer
40. Glucose sensor for low-cost lifetime-based sensing using a genetically engineered protein
41. Multicommutation in flow analysis. 6. Binary sampling for wide concentration range turbidimetric determination of sulphate in plant digests
42. Rational design of a calcium sensing system based on induced conformational changes of calmodulin
43. Effects of sulfate on selenate uptake and toxicity in the green alga Selenastrum capricomutum
44. Periplasmic binding protein based biosensors 1. Preliminary study of maltose binding protein as sensing element for maltose biosensor