Remedial strategies in structural proteomics: Expression, purification, and crystallization of the Vav1/Rac1 complex

Protein Expression and Purification

Volumn 53, Issue 1, 2007, Pages 51-62

  Brooun, Alexei ^a   Foster, Scott A ^a   Chrencik, Jill E ^a   Chien, Ellen Y T ^a   Kolatkar, Anand R ^a   Streiff, Markus ^b   Ramage, Paul ^b   Widmer, Hans ^b   Weckbecker, Gisbert ^b   Kuhn, Peter ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Cysteine rich segment; Guanine nucleotide exchange factor; Protein protein interaction; Structural proteomics; Surface mutagenesis

Indexed keywords

COMPLEMENTARY DNA; CYSTEINE; GLUTATHIONE TRANSFERASE; GUANINE NUCLEOTIDE EXCHANGE FACTOR; PROTEIN; RAC1 PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VAV PROTEIN; VAV1 PROTEIN, HUMAN; ZINC;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CRYSTALLIZATION; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTAGENESIS; NANOTECHNOLOGY; NUCLEAR MAGNETIC RESONANCE; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; PROTEOMICS;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; CRYSTALLIZATION; CYSTEINE; DNA, COMPLEMENTARY; GLUTATHIONE TRANSFERASE; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HYDROLYSIS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NANOTECHNOLOGY; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; PROTEOMICS; PROTO-ONCOGENE PROTEINS C-VAV; RAC1 GTP-BINDING PROTEIN; RECOMBINANT PROTEINS; ZINC;

EID: 33847157829     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.10.027     Document Type: Article

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