Inactivation of Rho GTPases by p190 RhoGAP reduces human pancreatic cancer cell invasion and metastasis

Cancer Science

Volumn 97, Issue 9, 2006, Pages 848-853

  Kusama, Toshiyuki ^a,b,c   Mukai, Mutsuko ^a   Endo, Hiroko ^a   Ishikawa, Osamu ^a   Tatsuta, Masaharu ^a   Nakamura, Hiroyuki ^a   Inoue, Masahiro ^a  

^a OSAKA MEDICAL CENTER FOR CANCER AND CARDIOVASCULAR DISEASES   (Japan)

^b Shinko Hospital   (Japan)

^c UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR; PROTEIN P190; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; RHOB GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; CANCER CELL; CARBOXY TERMINAL SEQUENCE; CELL INVASION; CHIMERA; CONTROLLED STUDY; ENZYME INHIBITION; GENE INACTIVATION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LIVER METASTASIS; METASTASIS POTENTIAL; PANCREAS CANCER; PRIORITY JOURNAL; PROTEIN DOMAIN;

ANIMALS; BLOTTING, WESTERN; CARRIER PROTEINS; CELL LINE, TUMOR; CHIMERA; DNA-BINDING PROTEINS; ENZYME ACTIVATION; EPIDERMAL GROWTH FACTOR; GTPASE-ACTIVATING PROTEINS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMANS; MICE; MITOGEN-ACTIVATED PROTEIN KINASES; NEOPLASM INVASIVENESS; NEOPLASM TRANSPLANTATION; NEOPLASMS, EXPERIMENTAL; PANCREATIC NEOPLASMS; REPRESSOR PROTEINS; RHO GTP-BINDING PROTEINS; TRANSFECTION;

EID: 33746648699     PISSN: 13479032     EISSN: 13497006     Source Type: Journal    
DOI: 10.1111/j.1349-7006.2006.00242.x     Document Type: Article

References (25)

1. Hall A. Rho GTPases and the actin cytoskeleton. Science 1998; 279: 509-14.
2. Etienne-Manneville S, Hall A. Rho GTPases in cell biology. Nature 2002; 420: 692-35.
3. Olofsson B. Rho guanine dissociation inhibitors: Pivotal molecules in cellular signalling. Cell Signal 1999; 11: 545-54.
4. Ridley AJ, Self AJ, Kasmi F et al. rho family GTPase activating proteins p190, bcr and rhoGAP show distinct specificities in vitro and in vivo. EMBO J 1993; 12: 5151-60.
5. Wang L, Yang L, Luo Y, Zheng Y. A novel strategy for specifically down-regulating individual Rho GTPase activity in tumor cells. J Biol Chem 2003; 278: 44617-25.
6. Kusama T, Mukai M, Iwasaki T et al. Inhibition of epidermal growth factor-induced RhoA translocation and invasion of human pancreatic cancer cells by 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors. Cancer Res 2001; 61: 4885-91.
7. Kusama T, Mukai M, Iwasaki T et al. 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors reduce human pancreatic cancer cell invasion and metastasis. Gastroenterology 2002; 122: 308-17.
8. Kusama T, Mukai M, Ayaki M et al. Inhibition of lysophosphatidic acid-induced RhoA activation and tumor cell invasion by 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors. Int J Oncol 2003; 23: 1173-8.
9. Kusama T, Mukai M, Tatsuta M, Matsumoto Y, Nakamura H, Inoue M. Selective inhibition of cancer cell invasion by a geranylgeranyltransferase-I inhibitor. Clin Exp Metastasis 2003; 20: 561-7.
10. Watanabe R, Kinoshita T, Masaki R, Yamamoto A, Takeda J, Inoue N. PIG-A and PIG-H, which participate in glycosylphosphatidylinositol anchor biosynthesis, form a protein complex in the endoplasmic reticulum. J Biol Chem 1996; 271: 26868-75.
11. Hamanaka Y, Mukai M, Shimamura M et al. Suppression of PI3K/mTOR pathway rescues LLC cells from cell death induced by hypoxia. Biochem Biophys Res Commun 2005; 330: 318-26.
12. Akagi T, Sasai K, Hanafusa H. Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation. Proc Natl Acad Sci USA 2003; 100: 13567-72.
13. Ren X-D, Kiosses WB, Schwartz MA. Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J 1999; 18: 578-85.
14. Zhang B, Zheng Y. Regulation of RhoA GTP hydrolysis by the GTPase-activating proteins p190, p50RhoGAP, Bcr, and 3BP-1. Biochemistry 1998; 37: 5249-57.
15. Settleman J, Narasimhan V, Foster LC, Weinberg RA. Molecular cloning of cDNAs encoding the GAP-associated protein p190: Implications for a signaling pathway from ras to the nucleus. Cell 1992; 69: 539-49.
16. Adamson P, Paterson HF, Hall A. Intracellular localization of the P21rho proteins. J Cell Biol 1992; 119: 617-27.
17. Michaelson D, Silletti J, Murphy G, D'Eustachio P, Rush M, Philips MR. Differential localization of Rho GTPases in live cells: Regulation by hypervariable regions and RhoGDI binding. J Cell Biol 2001; 152: 111-26.
18. Fidler IJ. The pathogenesis of cancer metastasis: The 'seed and soil' hypothesis revisited. Nat Rev Cancer 2003; 3: 453-8.
19. Olson MF, Ashworth A, Hall A. An essential role for Rho, Rac, and Cdc42 GTPases in cell cycle progression through G1. Science 1995; 269: 1270-2.
20. Noguchi Y, Nakamura S, Yasuda T et al. Newly synthesized Rho A, not Ras, is isoprenylated and translocated to membranes coincident with progression of the G1 to S phase of growth-stimulated rat FRTL-5 cells. J Biol Chem 1998; 273: 3649-53.
21. Wang DZ, Nur-E-Kamal MS, Tikoo A, Montague W, Maruta H. The GTPase and Rho GAP domains of p190, a tumor suppressor protein that binds the M(r) 120,000 Ras GAP, independently function as anti-Ras tumor suppressors. Cancer Res 1997; 57: 2478-84.
22. Suwa H, Ohshio G, Imamura T et al. Overexpression of the rhoC gene correlates with progression of ductal adenocarcinoma of the pancreas. Br J Cancer 1998; 77: 147-52.
23. Fritz G, Just I, Kaina B. Rho GTPases are over-expressed in human tumors. Int J Cancer 1999; 81: 682-7.
24. Kleer CG, van Golen KL, Zhang Y, Wu ZF, Rubin MA, Merajver SD. Characterization of RhoC expression in benign and malignant breast disease: A potential new marker for small breast carcinomas with metastatic ability. Am J Pathol 2002; 160: 579-84.
25. Elson CE, Peffley DM, Hentosh P, Mo H. Isoprenoid-mediated inhibition of mevalonate synthesis: Potential application to cancer. Proc Soc Exp Biol Med 1999; 221: 294-311.