Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes

RNA

Volumn 13, Issue 1, 2007, Pages 22-29

  Kim, Kevin ^a   Lee, Young Sik ^b   Carthew, Richard W ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b Korea University   (South Korea)

Author keywords

Ago2; Drosophila; RISC assembly; RNAi; siRNA

Indexed keywords

ARGONAUTE2 ENZYME; COMPLEMENTARY RNA; DOUBLE STRANDED RNA; ENDONUCLEASE; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; CONTROLLED STUDY; DROSOPHILA; ENZYME ACTIVITY; ENZYME ISOLATION; GENE IDENTIFICATION; GENE SILENCING; INSECT GENETICS; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE;

ALLELES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; DROSOPHILA; DROSOPHILA PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNA-INDUCED SILENCING COMPLEX;

EID: 33845890037     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.283207     Document Type: Article

References (22)

1. Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., and Mello, C.C. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811.
2. Lee, Y.S. and Carthew, R.W. 2003. Making a better RNAi vector for Drosophila: Use of intron spacers. Methods 30: 322-329.
3. Lee, Y.S., Nakahara, K., Pham, J.W., Kim, K., He, Z., Sontheimer, E.J., and Carthew, R.W. 2004. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 117: 69-81.
4. Leuschner, P.J., Ameres, S.L., Kueng, S., and Martinez, J. 2006. Cleavage of the siRNA passenger strand during RISC assembly in human cells. EMBO Rep. 7: 314-320.
5. Liu, Q., Rand, T.A., Kalidas, S., Du, F., Kim, H.E., Smith, D.P., and Wang, X. 2003. R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway. Science 301: 1921-1925.
6. Liu, J., Carmell, M.A., Rivas, F.V., Marsden, C.G., Thomson, J.M., Song, J.J., Hammond, S.M., Joshua-Tor, L., and Hannon, G.J. 2004. Argonaute2 is the catalytic engine of mammalian RNAi. Science 305: 1437-1441.
7. Matranga, C., Tomari, Y., Shin, C., Bartel, D.P., and Zamore, P.D. 2005. Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. Cell 123: 607-620.
8. Meister, G. and Tuschl, T. 2004. Mechanisms of gene silencing by double-stranded RNA. Nature 431: 343-349.
9. Newsome, T.P., Asling, B., and Dickson, B.J. 2000. Analysis of Drosophila photoreceptor axon guidance in eye-specific mosaics. Development 127: 851-860.
10. Nowotny, M., Gaidamakov, S.A., Crouch, R.J., and Yang, W. 2005. Crystal structures of RNase H bound to an RNA/DNA hybrid: Substrate specificity and metal-dependent catalysis. Cell 121: 1005-1016.
11. Nykanen, A., Haley, B., and Zamore, P.D. 2001. ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell 107: 309-321.
12. Okamura, K., Ishizuka, A., Siomi, H., and Siomi, M.C. 2004. Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. Genes & Dev. 18: 1655-1666.
13. Parker, J.S., Roe, S.M., and Barford, D. 2004. Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity. EMBO J. 23: 4727-4737.
14. Pham, J.W. and Sontheimer, E.J. 2005. Molecular requirements for RNA-induced silencing complex assembly in the Drosophila RNA interference pathway. J. Biol. Chem. 280: 39278-39283.
15. Pham, J.W., Pellino, J.L., Lee, Y.S., Carthew, R.W., and Sontheimer, E.J. 2004. A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila. Cell 117: 83-94.
16. Rand, T.A., Petersen, S., Du, F., and Wang, X. 2005. Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation. Cell 123: 621-629.
17. Rivas, F.V., Tolia, N.H., Song, J.J., Aragon, J.P., Liu, J., Hannon, G.J., and Joshua-Tor, L. 2005. Purified Argonaute2 and an siRNA form recombinant human RISC. Nat. Struct. Mol. Biol. 12: 340-349.
18. Song, J.J., Smith, S.K., Hannon, G.J., and Joshua-Tor, L. 2004. Crystal structure of Argonaute and its implications for RISC slicer activity. Science 305: 1434-1437.
19. Stowers, R.S. and Schwarz, T.L. 1999. A genetic method for generating Drosophila eyes composed exclusively of mitotic clones of a single genotype. Genetics 152: 1631-1639.
20. Tomari, Y., Du, T., Haley, B., Schwarz, D.S., Bennett, R., Cook, H.A., Koppetsch, B.S., Theurkauf, W.E., and Zamore, P.D. 2004a. RISC assembly defects in the Drosophila RNAi mutant armitage. Cell 116: 831-841.
21. Tomari, Y., Matranga, C., Haley, B., Martinez, N., and Zamore, P.D. 2004b. A protein sensor for siRNA asymmetry. Science 306: 1377-1380.
22. Yang, W. and Steitz, T.A. 1995. Recombining the structures of HIV integrase, RuvC and RNase H. Structure 3: 131-134.