An in vitro method for selective detection of free monomeric ubiquitin by using a C-terminally biotinylated form of ubiquitin

International Journal of Biochemistry and Cell Biology

Volumn 39, Issue 2, 2007, Pages 319-326

  Staszczak, Magdalena ^a  

^a MARIA CURIE SK ODOWSKA UNIVERSITY   (Poland)

Author keywords

Biotinylation; Ubiquitin; Ubiquitin detection

Indexed keywords

AVIDIN; CELL EXTRACT; HORSERADISH PEROXIDASE; LIGASE; MONOMER; POLYUBIQUITIN; PROTEASOME; STREPTAVIDIN; UBIQUITIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; CELL FREE SYSTEM; IN VITRO STUDY; NONHUMAN; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; UBIQUITINATION; WESTERN BLOTTING;

ANIMALS; AVIDIN; BIOTINYLATION; BLOTTING, WESTERN; CELL-FREE SYSTEM; CHROMATOGRAPHY, AFFINITY; PEPTIDES; POLYUBIQUITIN; RABBITS; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY; UBIQUITIN; UBIQUITINS;

ARMORACIA RUSTICANA;

EID: 33845219523     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biocel.2006.08.013     Document Type: Article

References (30)

1. Asher G., Lotem J., Sachs L., et al. Mdm-2 and ubiquitin-independent p53 proteasomal degradation regulated by NQO1. Proceedings of the National Academy of Sciences of the United States of America 99 (2002) 13125-13130
2. Attaix D., Combaret L., Pouch M.-N., et al. Cellular control of ubiquitin-proteasome-dependent proteolysis. Journal of Animal Science 80 E. Suppl. 2 (2002) E56-E63
3. Baumeister W., and Zwickl P. The 26S proteasome: A supramolecular assembly designed for controlled proteolysis. In: Ciechanover A.J., and Masucci M.G. (Eds). The ubiquitin-proteasome proteolytic system: From classical biochemistry to human diseases, recent advances in human biology Vol 9 (2002), World Scientific, River Edge, NJ 67-80
4. Beckett D., Kovaleva E., and Schatz P.J. A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation. Protein Science 8 (1999) 921-929
5. Bloom J., and Pagano M. Experimental tests to definitively determine ubiquitinylation of a substrate. Methods in Enzymology 399 (2005) 249-266
6. Chapman-Smith A., and Cronan Jr. J.E. The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity. Trends in Biochemical Sciences 24 (1999) 359-363
7. Chen I., Howarth M., Lin W., and Ting A.Y. Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature Methods 2 (2005) 99-104
8. Chen Z., and Pickart C.M. A 25-kilodalton ubiquitin carrier protein (E2) catalyzes multi-ubiquitin chain synthesis via lysine 48 of ubiquitin. Journal of Biological Chemistry 265 (1990) 21835-21842
9. Ciechanover A., Hod Y., and Hershko A. A heat-stable component of an ATP-dependent proteolytic system from reticulocytes. Biochemical and Biophysical Research Communications 81 (1978) 1100-1105
10. Corsi D., Galluzzi L., Crinelli R., et al. Ubiquitin is conjugated to the cytoskeletal protein α-spectrin in mature erythrocytes. Journal of Biological Chemistry 270 (1995) 8928-8935
11. Dantuma N.P., Groothuis T.A.M., Salomons F.A., et al. A dynamic ubiquitin equilibrium couples proteasomal activity to chromatin remodeling. Journal of Cell Biology 173 (2006) 19-26
12. Fujimuro M., Sawada H., and Yokosawa H. Production and characterization of monoclonal antibodies specific to multiubiquitinated chains of polyubiquitinated proteins. FEBS Letters 349 (1994) 173-180
13. Glickman M.H., and Ciechanover A. The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiological Reviews 82 (2002) 373-428
14. Haas A.L., and Bright P.M. The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates. Journal of Biological Chemistry 260 (1985) 12464-12473
15. Hershko A., and Ciechanover A. The ubiquitin system. Annual Review of Biochemistry 67 (1998) 425-479
16. Hershko A., Heller H., Elias S., et al. Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown. Journal of Biological Chemistry 258 (1983) 8206-8214
17. Lam Y.A., DeMartino G.N., Pickart C.M., et al. Specificity of the ubiquitin isopeptidase in the PA700 regulatory complex of 26S proteasomes. Journal of Biological Chemistry 272 (1997) 28438-28446
18. Mimnaugh E.G., Bonvini P., and Neckers L. The measurement of ubiquitin and ubiquitinated proteins. Electrophoresis 20 (1999) 418-428
19. Mimnaugh E.G., and Neckers L.M. Immunoblotting methods for the study of protein ubiquitination. Methods in Molecular Biology 194 (2002) 179-203
20. Perroy J., Pontier S., Charest P.G., et al. Real-time monitoring of ubiquitination in living cells by BRET. Nature Methods 1 (2004) 203-208
21. Pickart C.M., and Cohen R.E. Proteasomes and their kin: Proteases in the machine age. Nature Reviews Molecular Cell Biology 5 (2004) 177-187
22. Piotrowski J., Beal R., Hoffman L., et al. Inhibition of the 26 S proteasome by polyubiquitin chains synthesized to have defined lengths. Journal of Biological Chemistry 272 (1997) 23712-23721
23. Pouny Y., Weitzman C., and Kaback H.R. In vitro biotinylation provides quantitative recovery of highly purified active lactose permease in a single step. Biochemistry 37 (1998) 15713-15719
24. Takada K., Hibi N., Tsukada Y., et al. Ability of ubiquitin radioimmunoassay to discriminate between monoubiquitin and multi-ubiquitin chains. Biochimica et Biophysica Acta 1290 (1996) 282-288
25. 1-antitrypsin Z in the endoplasmic reticulum. American Journal of Physiology - Gastrointestinal and Liver Physiology 278 (2000) G39-G48
26. Wang T., Evdokimov E., Yiadom K., et al. Biotin-ubiquitin tagging of mammalian proteins in Escherichia coli. Protein Expression and Purification 30 (2003) 140-149
27. Whitby F.G., Xia G., Pickart C.M., and Hill C.P. Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes. Journal of Biological Chemistry 273 (1998) 34983-34991
28. Wilkinson K. Ubiquitination and deubiquitination: targeting of proteins for degradation by the proteasome. Seminars in Cell and Developmental Biology 11 (2000) 141-148
29. Wilkinson K.D., and Hochstrasser M. The deubiquitinating enzymes. In: Peters J.-M., Harris J.R., and Finley D. (Eds). Ubiquitin and the biology of the cell (1998), Plenum Press, New York 99-125
30. Xu Y., and Beckett D. Biotinyl-5′-adenylate synthesis catalyzed by Escherichia coli repressor of biotin biosynthesis. Methods in Enzymology 279 (1999) 405-421