Real-time monitoring of ubiquitination in living cells by BRET

Nature Methods

Volumn 1, Issue 3, 2004, Pages 203-208

  Perroy, Julie ^a,b   Pontier, Stephanie ^a   Charest, Pascale G ^a   Aubry, Muriel ^a   Bouvier, Michel ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b INSTITUT DE GÉNOMIQUE FONCTIONNELLE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BETA ARRESTIN; G PROTEIN COUPLED RECEPTOR; PHOTOPROTEIN; RETINA S ANTIGEN; UBIQUITIN;

ARTICLE; CELL LINE; COMPARATIVE STUDY; COMPUTER SYSTEM; EVALUATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; KIDNEY; KINETICS; LUMINESCENCE; METABOLIC CLEARANCE RATE; METABOLISM; METHODOLOGY; PHYSIOLOGY; PRENATAL DEVELOPMENT; PROTEIN ANALYSIS; PROTEIN PROCESSING; VALIDATION STUDY;

ARRESTINS; CELL LINE; COMPUTER SYSTEMS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; KIDNEY; KINETICS; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; METABOLIC CLEARANCE RATE; PROTEIN INTERACTION MAPPING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECEPTORS, G-PROTEIN-COUPLED; UBIQUITINS;

EID: 12344276574     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth722     Document Type: Article

References (22)

1. Ciechanover, A., Orian, A. & Schwartz, A.L. The ubiquitin-mediated proteolytic pathway: Mode of action and clinical implications. J. Cell Biochem. Suppl. 34, 40–51 (2000).
2. Passmore, L.A. & Barford, D. Getting into position: The catalytic mechanisms of protein ubiquitylation. Biochem. J. 379 (Part 3), 513–525 (2004).
3. Hochstrasser, M. Ubiquitin-dependent protein degradation. Annu. Rev. Genet. 30, 405–439 (1996).
4. Schnell, J.D. & Hicke, L. Non-traditional functions of ubiquitin and ubiquitinbinding proteins. J. Biol. Chem. 278, 35857–35860 (2003).
5. Eidne, K.A., Kroeger, K.M. & Hanyaloglu, A.C. Applications of novel resonance energy transfer techniques to study dynamic hormone receptor interactions in living cells. Trends Endocrinol. Metab. 13, 415–421 (2002).
6. Wojcikiewicz, R.J. Regulated ubiquitination of proteins in GPCR-initiated signaling pathways. Trends Pharmacol. Sci. 25, 35–41 (2004).
7. Shenoy, S.K., McDonald, P.H., Kohout, T.A. & Lefkowitz, R.J. Regulation of receptor fate by ubiquitination of activated beta 2-adrenergic receptor and beta-arrestin. Science 294, 1307–1313 (2001).
8. Oakley, R.H., Laporte, S.A., Holt, J.A., Barak, L.S. & Caron, M.G. Association of beta-arrestin with G protein-coupled receptors during clathrin-mediated endocytosis dictates the profile of receptor resensitization. J. Biol. Chem. 274, 32248–32257 (1999).
9. Oakley, R.H., Laporte, S.A., Holt, J.A., Caron, M.G. & Barak, L.S. Differential affinities of visual arrestin, beta-arrestin 1, and beta-arrestin 2 for G proteincoupled receptors delineate two major classes of receptors. J. Biol. Chem. 275, 17201–17210 (2000).
10. Shenoy, S.K. & Lefkowitz, R.J. Trafficking patterns of beta-arrestin and G proteincoupled receptors determined by the kinetics of beta-arrestin deubiquitination. J. Biol. Chem. 278, 14498–14506 (2003).
11. Patrick, G.N., Bingol, B., Weld, H.A. & Schuman, E.M. Ubiquitin-mediated proteasome activity is required for agonist-induced endocytosis of GluRs. Curr. Biol. 13, 2073–2081 (2003).
12. Mercier, J.F., Salahpour, A., Angers, S., Breit, A. & Bouvier, M. Quantitative assessment of beta 1- and beta 2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer. J. Biol. Chem. 277, 44925–44931 (2002).
13. Lavoie, C. et al. Beta 1/beta 2-adrenergic receptor heterodimerization regulates beta 2-adrenergic receptor internalization and ERK signaling efficacy. J. Biol. Chem. 277, 35402–35410 (2002).
14. Jensen, A.A., Hansen, J.L., Sheikh, S.P. & Brauner-Osborne, H. Probing intermolecular protein-protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET). Eur. J. Biochem. 269, 5076–5087 (2002).
15. Angers, S. et al. Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc. Natl. Acad. Sci. USA 97, 3684–3689 (2000).
16. Xu, Y., Piston, D.W. & Johnson, C.H. A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins. Proc. Natl. Acad. Sci. USA 96, 151–156 (1999).
17. Haas, A.L., Murphy, K.E. & Bright, P.M. The inactivation of ubiquitin accounts for the inability to demonstrate ATP, ubiquitin-dependent proteolysis in liver extracts. J. Biol. Chem. 260, 4694–4703 (1985).
18. Wilkinson, K.D. & Audhya, T.K. Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly. J. Biol. Chem. 256, 9235–9241 (1981).
19. Perroy, J., Adam, L., Qanbar, R., Chenier, S. & Bouvier, M. Phosphorylation-independent desensitization of GABA(B) receptor by GRK4. EMBO J. 22, 3816–3824 (2003).
20. Morello, J.P. et al. Pharmacological chaperones rescue cell-surface expression and function of misfolded V2 vasopressin receptor mutants. J. Clin. Invest. 105, 887–895 (2000).
21. Terrillon, S. et al. Oxytocin and vasopressin V1a and V2 receptors form constitutive homo- and hetero-dimers during biosynthesis. Mol. Endocrinol. 17, 677–691 (2003).
22. Mellon, P., Parker, V., Gluzman, Y. & Maniatis, T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell 27, 279–288 (1981).