In vitro biotinylation provides quantitative recovery of highly purified active lactose permease in a single step

Biochemistry

Volumn 37, Issue 45, 1998, Pages 15713-15719

  Pouny, Yehonathan ^a   Weitzman, Cindy ^a   Ronald Kaback, H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; AVIDIN; CARBOXYLYASE; LACTOSE PERMEASE; LIGASE; MEMBRANE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; BIOTINYLATION; CHEMICAL MODIFICATION; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN;

ANILINO NAPHTHALENESULFONATES; BINDING SITES; BIOLOGICAL TRANSPORT; BIOTINYLATION; CHROMATOGRAPHY, AFFINITY; ENZYME ACTIVATION; ESCHERICHIA COLI PROTEINS; LIGANDS; LIPOSOMES; MEMBRANE TRANSPORT PROTEINS; MONOSACCHARIDE TRANSPORT PROTEINS; SPECTROMETRY, FLUORESCENCE; SYMPORTERS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; NEGIBACTERIA;

EID: 0032506039     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi981519z     Document Type: Article

References (13)

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