Experimental tests to definitively determine ubiquitylation of a substrate

Methods in Enzymology

Volumn 399, Issue , 2005, Pages 249-266

  Bloom, Joanna ^a   Pagano, Michele ^a  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CONJUGATION; CONTROLLED STUDY; EMBRYO; ENZYME SUBSTRATE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; UBIQUITINATION; WESTERN BLOTTING;

EID: 28844468834     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)99017-4     Document Type: Review

References (26)

1. Aviel S., Winberg G., Massucci M., and Ciechanover A. Degradation of the Epstein-Barr virus latent membrane protein 1 (LMP1) by the ubiquitin-proteasome pathway. Targeting via ubiquitination of the N-terminal residue J. Biol. Chem. 275 2000 23491 23499
2. Bashir T., Dorrello N.V., Amador V., Guardavaccaro D., and Pagano M. Control of the SCF(Skp2-Cks1) ubiquitin ligase by the APC/C(Cdh1) ubiquitin ligase Nature 428 2004 190 193
3. Ben-Saadon R., Fajerman I., Ziv T., Hellman U., Schwartz A.L., and Ciechanover A. The tumor suppressor protein p16INK4a and the human papillomavirus oncoprotein E7-58 are naturally occurring lysine-less proteins that are degraded by the ubiquitin system: Direct evidence for ubiquitination at the N-terminal residue J. Biol. Chem. 279 2004 41414 41421
4. Bloom J., Amador V., Bartolini F., DeMartino G., and Pagano M. Proteasome-mediated degradation of p21 via N-terminal ubiquitinylation Cell 115 2003 71 82
5. Breitschopf K., Bengal E., Ziv T., Admon A., and Ciechanover A. A novel site for ubiquitination: The N-terminal residue, and not internal lysines of MyoD, is essential for conjugation and degradation of the protein EMBO J. 17 1998 5964 5973
6. Carrano A.C., Eytan E., Hershko A., and Pagano M. SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27 Nat. Cell Biol. 1 1999 193 199
7. Cope G.A., and Deshaies R.J. COP9 signalosome: A multifunctional regulator of SCF and other cullin-based ubiquitin ligases Cell 114 2004 663 671
8. Coulombe P., Rodier G., Bonneil E., Thibault P., and Meloche S. N-terminal ubiquitination of extracellular signal-regulated kinase 3 and p21 directs their degradation by the proteasome Mol. Cell Biol. 24 2004 6140 6150
9. Ganoth D., Bornstein G., Ko T.K., Larsen B., Tyers M., Pagano M., and Hershko A. The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27 Nat. Cell Biol. 3 2001 321 324
10. Hershko A. Ubiquitin-mediated protein degradation J. Biol. Chem. 263 1988 15237 15240
11. Hershko A., and Ciechanover A. The ubiquitin system Annu. Rev. Biochem. 67 1998 425 479
12. Ikeda M., Ikeda A., and Longnecker R. Lysine-independent ubiquitination of Epstein-Barr virus LMP2A Virology 300 2002 153 159
13. Kuo M.L., den Besten W., Bertwistle D., Roussel M.F., and Sherr C.J. N-terminal polyubiquitination and degradation of the Arf tumor suppressor Genes Dev. 18 2004 1862 1874
14. Montagnoli A., Fiore F., Eytan E., Carrano A.C., Draetta G.F., Hershko A., and Pagano M. Ubiquitination of p27 is regulated by Cdk-dependent phosphorylation and trimeric complex formation Genes Dev. 13 1999 1181 1189
15. Moren A., Hellman U., Inada Y., Imamura T., Heldin C.H., and Moustakas A. Differential ubiquitination defines the functional status of the tumor suppressor Smad4 J. Biol. Chem. 278 2003 33571 33582
16. Nishimoto T., Takahashi T., and Basilico C. A temperature-sensitive mutation affecting S-phase progression can lead to accumulation of cells with a G2 DNA content Somatic Cell Genet. 6 1980 465 476
17. Pagano M., Tam S.W., Theodoras A.M., Beer-Romero P., Del Sal S., Chau V., Yew P.R., Draetta G.F., and Rolfe M. Role of the ubiquitin-proteasome pathway in regulating abundance of the cyclin-dependent kinase inhibitor p27 Science 269 1995 682 685
18. Pan Z.Q., Kentsis A., Dias D.C., Yamoah K., and Wu K. Nedd8 on cullin: Building an expressway to protein destruction Oncogene 23 2004 1985 1997
19. Pickart C.M. Mechanisms underlying ubiquitination Annu. Rev. Biochem. 70 2001 503 533
20. Pickart C.M. Back to the future with ubiquitin Cell 116 2004 181 190
21. Reinstein E., Scheffner M., Oren M., Ciechanover A., and Schwartz A. Degradation of the E7 human papillomavirus oncoprotein by the ubiquitin-proteasome system: Targeting via ubiquitination of the N-terminal residue Oncogene 19 2000 5944 5950
22. Rolfe M. Stable and transient transfections in cell cycle studies M. Pagano "Cell cycle: Materials and methods" 1995 Springer-Verlag New York 87 92
23. Seeler J.S., and Dejean A. Nuclear and unclear functions of SUMO Nat. Rev. Mol. Cell Biol. 4 2003 690 699
24. Treier M., Staszewski L.M., and Bohmann D. Ubiquitin-dependent c-Jun degradation in vivo is mediated by the delta domain Cell 78 1994 787 798
25. Wei W., Ayad N.G., Wan Y., Zhang G.J., Kirschner M.W., and Kaelin W.G. Jr. Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase-promoting complex Nature 428 2004 194 198
26. Yaron A., Hatzubai A., Davis M., Lavon I., Amit S., Manning A.M., Andersen J.S., Mann M., Mercurio F., and Ben-Neriah Y. Identification of the receptor component of the IkappaBalpha-ubiquitin ligase Nature 396 1998 590 594