Biotin-ubiquitin tagging of mammalian proteins in Escherichia coli

Protein Expression and Purification

Volumn 30, Issue 1, 2003, Pages 140-149

  Wang, Tao ^a   Evdokimov, Evgenij ^a   Yiadom, Kwabena ^a   Yan, Zhengyin ^b   Chock, P Boon ^c   Yang, David C H ^a  

^a GEORGETOWN UNIVERSITY   (United States)

^b RW Johnson Pharmaceutical Research Institute   (United States)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; MAMMALIA;

EID: 0038010121     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(03)00098-6     Document Type: Article

References (45)

1. D. Finley, B. Bartel, A. Varshavsky, The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis, Nature 338 (1989) 394-401.
2. R. Barrio, A. del Arco, H.L. Cabrera, C. Arribas, Structure and expression of the Drosophila ubiquitin-80-amino-acid fusion-protein gene, Biochem. J. 302 (1994) 237-244.
3. L. Liu, D.S. Maillet, J.R. Frappier, K. d'Ailly, D.B. Walden, B.G. Atkinson, Characterization, chromosomal mapping, and expression of different ubiquitin fusion protein genes in tissues from control and heat-shocked maize seedlings, Biochem. Cell Biol. 74 (1996) 9-19.
4. A. Hershko, A. Ciechanover, The ubiquitin system, Annu. Rev. Biochem. 67 (1998) 425-479.
5. C.M. Pickart, Mechanisms underlying ubiquitination, Annu. Rev. Biochem. 70 (2001) 503-533.
6. A.M. Weissman, Ubiquitin and proteasomes: themes and variations on ubiquitylation, Nat. Rev. Mol. Cell Biol. 2 (2001) 169-178.
7. D. Hondred, J.M. Walker, D.E. Mathews, R.D. Vierstra, Use of ubiquitin fusions to augment protein expression in transgenic plants, Plant Physiol. 119 (1999) 713-724.
8. A.H. Christensen, P.H. Quail, Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants, Transgenic Res. 5 (1996) 213-218.
9. T.R. Butt, M.I. Khan, J. Marsh, D.J. Ecker, S.T. Crooke, Ubiquitin-metallothionein fusion protein expression in yeast. A genetic approach for analysis of ubiquitin functions, J. Biol. Chem. 263 (1988) 16364-16371.
10. D.J. Ecker, J.M. Stadel, T.R. Butt, J.A. Marsh, B.P. Monia, D.A. Powers, J.A. Gorman, P.E. Clark, F. Warren, A. Shatzman, S.T. Crooke, Increasing gene expression in yeast by fusion to ubiquitin, J. Biol. Chem. 264 (1989) 7715-7719.
11. A.L. Pilon, P. Yost, T.E. Chase, G.L. Lohnas, W.E. Bentley, High-level expression and efficient recovery of ubiquitin fusion proteins from Escherichia coli, Biotechnol. Prog. 12 (1996) 331-337.
12. B.W. Cherney, B. Chaudhry, K. Bhatia, T.R. Butt, M. Smulson, Expression and mutagenesis of human poly(ADP-ribose) polymerase as a ubiquitin fusion protein from Escherichia coli, Biochemistry 30 (1991) 10420-10427.
13. J.L. Wittliff, L.L. Wenz, J. Dong, Z. Nawaz, T.R. Butt, Expression and characterization of an active human estrogen receptor as a ubiquitin fusion protein from Escherichia coli, J. Biol. Chem. 265 (1990) 22016-22022.
14. T. Wang, P.B. Chock, D.C.H. Yang, Expression of biotinylated ubiquitin fusion proteins in E. coli, FASEB J. 14 (2000) a1531.
15. J.W. Tobias, A. Varshavsky, Cloning and functional analysis of the ubiquitin-specific protease gene UBP1 of Saccharomyces cerevisiae, J. Biol. Chem. 266 (1991) 12021-12028.
16. K.D. Wilkinson, K.M. Lee, S. Deshpande, P. Duerksen-Hughes, J.M. Boss, J. Pohl, The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase, Science 246 (1989) 670-673.
17. E.T. Yeh, L. Gong, T. Kamitani, Ubiquitin-like proteins: new wines in new bottles, Gene 248 (2000) 1-14.
18. S. Muller, C. Hoege, G. Pyrowolakis, S. Jentsch, SUMO, ubiquitin's mysterious cousin, Nat. Rev. Mol. Cell Biol. 2 (2001) 202-210.
19. M. Hochstrasser, SP-RING for SUMO: new functions bloom for a ubiquitin-like protein, Cell 107 (2001) 5-8.
20. V.G. Wilson, D. Rangasamy, Intracellular targeting of proteins by sumoylation, Exp. Cell Res. 271 (2001) 57-65.
21. J.S. Seeler, A. Dejean, SUMO: of branched proteins and nuclear bodies, Oncogene 20 (2001) 7243-7249.
22. S. Sachdev, L. Bruhn, H. Sieber, A. Pichler, F. Melchior, R. Grosschedl, PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies, Genes Dev. 15 (2001) 3088-3103.
23. P.J. Schatz, Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli, Biotechnology (N Y) 11 (1993) 1138-1143.
24. P.A. Smith, B.C. Tripp, E.A. DiBlasio-Smith, Z. Lu, E.R. LaVallie, J.M. McCoy, A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli, Nucleic Acids Res. 26 (1998) 1414-1420.
25. T. Wang, C.M. Simbulan-Rosenthal, Z. Yan, M. Smulson, P.B. Chock, D.C. Yang, Expression of in vivo biotinylated ubiquitin in E. coli and ubiquitinylation of poly(ADP-ribose) polymerase, 2003, submitted.
26. S.H. Baek, K.S. Choi, Y.J. Yoo, J.M. Cho, R.T. Baker, K. Tanaka, C.H. Chung, Molecular cloning of a novel ubiquitin-specific protease, UBP41, with isopeptidase activity in chick skeletal muscle, J. Biol. Chem. 272 (1997) 25560-25565.
27. S.H. Baek, K.C. Park, J.I. Lee, K.I. Kim, Y.J. Yoo, K. Tanaka, R.T. Baker, C.H. Chung, A novel family of ubiquitin-specific proteases in chick skeletal muscle with distinct N- and C-terminal extensions, Biochem. J. 334 (1998) 677-684.
28. A. Gewies, S. Grimm, UBP41 is a proapoptotic ubiquitin-specific protease, Cancer Res. 63 (2003) 682-688.
29. M.L. Hermida-Matsumoto, P.B. Chock, T. Curran, D.C. Yang, Ubiquitinylation of transcription factors c-Jun and c-Fos using reconstituted ubiquitinylating enzymes, J. Biol. Chem. 271 (1996) 4930-4936.
30. A. Hershko, H. Heller, S. Elias, A. Ciechanover, Components of ubiquitin protein ligase system: resolution, affinity purification, and role in protein breakdown, J. Biol. Chem. 258 (1983) 8206-8214.
31. R. Hammamieh, D.C. Yang, Magnesium ion-mediated binding to tRNA by an amino-terminal peptide of a class II tRNA synthetase, J. Biol. Chem. 276 (2001) 428-433.
32. G.W. Lee, F. Melchior, M.J. Matunis, R. Mahajan, Q. Tian, P. Anderson, Modification of Ran GTPase-activating protein by the small ubiquitin-related modifier SUMO-1 requires Ubc9, an E2-type ubiquitin-conjugating enzyme homologue, J. Biol. Chem. 273 (1998) 6503-6507.
33. Z. Yan, G.W. Caldwell, P.A. McDonell, W.J. Jones, A. August, J.A. Masucci, Mass spectrometric determination of a novel modification of the N-terminus of histidine-tagged proteins expressed in bacteria, Biochem. Biophys. Res. Commun. 259 (1999) 271-282.
34. C. Escalante, D.C. Yang, Expression of human aspartyl-tRNA synthetase in Escherichia coli. Functional analysis of the N-terminal putative amphiphilic helix, J. Biol. Chem. 268 (1993) 6014-6023.
35. B. Sun, K. Jeyaseelan, M.C. Chung, T.S. Teo, Rabbit ubiquitin-activating enzyme El: cDNA cloning, sequence and expression, Gene 196 (1997) 19-23.
36. K.D. Wilkinson, Ubiquitination and deubiquitination: targeting of proteins for degradation by the proteasome, Semin. Cell Dev. Biol. 11 (2000) 141-148.
37. A. Varshavsky, Ubiquitin fusion technique and its descendants, Methods Enzymol. 327 (2000) 578-593.
38. J.E. Cronan Jr., Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins, J. Biol. Chem. 265 (1990) 10327-10333.
39. R.T. Baker, Protein expression using ubiquitin fusion and cleavage, Curr. Opin. Biotechnol. 7 (1996) 541-546.
40. J.H. Park, E.A. Choi, E.W. Cho, K.S. Hahm, K.L. Kim, Maltose binding protein (MBP) fusion proteins with low or no affinity to amylose resins can be single-step purified using a novel anti-MBP monoclonal antibody, Mol. Cells 8 (1998) 709-716.
41. A. Chapman-Smith, D.L. Turner, J.E. Cronan Jr., T.W. Morris, J.C. Wallace, Expression, biotinylation and purification of a biotin-domain peptide from the biotin carboxyl carrier protein of Escherichia coli acetyl-CoA carhoxylase, Biochem J. 302 (Part 3) (1994) 881-887.
42. N.G. Hentz, L.G. Bachas, Fluorophore-linked assays for high-performance liquid chromatography postcolumn reaction detection of biotin and biocytin, Methods Enzymol. 279 (1997) 275-286.
43. M.J. Dunn, Detection of proteins on blots using the avidin-biotin system, Methods Mol. Biol. 32 (1994) 227-232.
44. H.I. Guy, D.R. Evans, Cloning and expression of the mammalian multifunctional protein CAD in Escherichia coli. Characterization of the recombinant protein and a deletion mutant lacking the major interdomain linker, J. Biol. Chem. 269 (1994) 23808-23816.
45. J.O. Koopmann, J. Blackburn, High affinity capture surface for matrix-assisted laser desorption/ionization compatible protein microarrays, Rapid Commun. Mass Spectrum 17 (2003) 455-462.