-
1
-
-
0031854986
-
Man-made cell-like compartments for molecular evolution
-
Tawfik D.S., and Griffiths A.D. Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 16 (1998) 652-656
-
(1998)
Nat. Biotechnol.
, vol.16
, pp. 652-656
-
-
Tawfik, D.S.1
Griffiths, A.D.2
-
2
-
-
23044447796
-
New genotype-phenotype linkages for directed evolution of functional proteins
-
Leemhuis H., et al. New genotype-phenotype linkages for directed evolution of functional proteins. Curr. Opin. Struct. Biol. 15 (2005) 472-478
-
(2005)
Curr. Opin. Struct. Biol.
, vol.15
, pp. 472-478
-
-
Leemhuis, H.1
-
3
-
-
1842530257
-
Flow cytometry: a new method to investigate the properties of water-in-oil-in-water emulsions
-
Hai M., et al. Flow cytometry: a new method to investigate the properties of water-in-oil-in-water emulsions. Langmuir 20 (2004) 2081-2085
-
(2004)
Langmuir
, vol.20
, pp. 2081-2085
-
-
Hai, M.1
-
4
-
-
33744930710
-
Electric control of droplets in microfluidic devices
-
Link D.R., et al. Electric control of droplets in microfluidic devices. Angew. Chem. Int. Ed. Engl. 45 (2006) 2556-2560
-
(2006)
Angew. Chem. Int. Ed. Engl.
, vol.45
, pp. 2556-2560
-
-
Link, D.R.1
-
5
-
-
33745423084
-
Overview: methods and applications for droplet compartmentalization of biology
-
Leamon J.H., et al. Overview: methods and applications for droplet compartmentalization of biology. Nat. Methods 3 (2006) 541-543
-
(2006)
Nat. Methods
, vol.3
, pp. 541-543
-
-
Leamon, J.H.1
-
6
-
-
25844472658
-
Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization
-
Levy M., et al. Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization. RNA 11 (2005) 1555-1562
-
(2005)
RNA
, vol.11
, pp. 1555-1562
-
-
Levy, M.1
-
7
-
-
28844466075
-
High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments
-
Aharoni A., et al. High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. Chem. Biol. 12 (2005) 1281-1289
-
(2005)
Chem. Biol.
, vol.12
, pp. 1281-1289
-
-
Aharoni, A.1
-
8
-
-
28044468397
-
Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization
-
Agresti J.J., et al. Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization. Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 16170-16175
-
(2005)
Proc. Natl. Acad. Sci. U. S. A.
, vol.102
, pp. 16170-16175
-
-
Agresti, J.J.1
-
9
-
-
2542525398
-
Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution
-
Ghadessy F.J., et al. Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution. Nat. Biotechnol. 22 (2004) 755-759
-
(2004)
Nat. Biotechnol.
, vol.22
, pp. 755-759
-
-
Ghadessy, F.J.1
-
10
-
-
27644566231
-
Cell-free selection of zinc finger DNA-binding proteins using in vitro compartmentalization
-
Sepp A., and Choo Y. Cell-free selection of zinc finger DNA-binding proteins using in vitro compartmentalization. J. Mol. Biol. 354 (2005) 212-219
-
(2005)
J. Mol. Biol.
, vol.354
, pp. 212-219
-
-
Sepp, A.1
Choo, Y.2
-
11
-
-
11844273937
-
Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery
-
Bernath K., et al. Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery. J. Mol. Biol. 345 (2005) 1015-1026
-
(2005)
J. Mol. Biol.
, vol.345
, pp. 1015-1026
-
-
Bernath, K.1
-
12
-
-
27144511241
-
Engineering novel binding proteins from nonimmunoglobulin domains
-
Binz H.K., et al. Engineering novel binding proteins from nonimmunoglobulin domains. Nat. Biotechnol. 23 (2005) 1257-1268
-
(2005)
Nat. Biotechnol.
, vol.23
, pp. 1257-1268
-
-
Binz, H.K.1
-
13
-
-
0037235949
-
Engineered antibodies
-
Hudson P.J., and Souriau C. Engineered antibodies. Nat. Med. 9 (2003) 129-134
-
(2003)
Nat. Med.
, vol.9
, pp. 129-134
-
-
Hudson, P.J.1
Souriau, C.2
-
14
-
-
13844250741
-
High-throughput screens and selections of enzyme-encoding genes
-
Aharoni A., et al. High-throughput screens and selections of enzyme-encoding genes. Curr. Opin. Chem. Biol. 9 (2005) 210-216
-
(2005)
Curr. Opin. Chem. Biol.
, vol.9
, pp. 210-216
-
-
Aharoni, A.1
-
15
-
-
33745339106
-
Directed evolution by in vitro compartmentalization
-
Miller O.J., et al. Directed evolution by in vitro compartmentalization. Nat. Methods 3 (2006) 561-570
-
(2006)
Nat. Methods
, vol.3
, pp. 561-570
-
-
Miller, O.J.1
-
16
-
-
0346728679
-
In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence-activated cell sorting
-
Bernath K., et al. In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence-activated cell sorting. Anal. Biochem. 325 (2004) 151-157
-
(2004)
Anal. Biochem.
, vol.325
, pp. 151-157
-
-
Bernath, K.1
-
17
-
-
0141905882
-
DNA display for in vitro selection of diverse peptide libraries
-
Yonezawa M., et al. DNA display for in vitro selection of diverse peptide libraries. Nucleic Acids Res. 31 (2003) e118
-
(2003)
Nucleic Acids Res.
, vol.31
-
-
Yonezawa, M.1
-
18
-
-
0035836707
-
Directed evolution of polymerase function by compartmentalized self-replication
-
Ghadessy F.J., et al. Directed evolution of polymerase function by compartmentalized self-replication. Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 4552-4557
-
(2001)
Proc. Natl. Acad. Sci. U. S. A.
, vol.98
, pp. 4552-4557
-
-
Ghadessy, F.J.1
-
19
-
-
2942654776
-
A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system
-
Ghadessy F.J., and Holliger P. A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system. Protein Eng. Des. Sel. 17 (2004) 201-204
-
(2004)
Protein Eng. Des. Sel.
, vol.17
, pp. 201-204
-
-
Ghadessy, F.J.1
Holliger, P.2
-
20
-
-
23044504786
-
Protein synthesis by pure translation systems
-
Shimizu Y., et al. Protein synthesis by pure translation systems. Methods 36 (2005) 299-304
-
(2005)
Methods
, vol.36
, pp. 299-304
-
-
Shimizu, Y.1
-
21
-
-
28844487473
-
High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions
-
Mastrobattista E., et al. High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions. Chem. Biol. 12 (2005) 1291-1300
-
(2005)
Chem. Biol.
, vol.12
, pp. 1291-1300
-
-
Mastrobattista, E.1
-
22
-
-
20544449855
-
Why high error-rate random mutagenesis libraries are enriched in functional and improved proteins
-
Drummond D.A., et al. Why high error-rate random mutagenesis libraries are enriched in functional and improved proteins. J. Mol. Biol. 350 (2005) 806-816
-
(2005)
J. Mol. Biol.
, vol.350
, pp. 806-816
-
-
Drummond, D.A.1
-
23
-
-
33745351130
-
Amplification of complex gene libraries by emulsion PCR
-
Williams R., et al. Amplification of complex gene libraries by emulsion PCR. Nat. Methods 3 (2006) 545-550
-
(2006)
Nat. Methods
, vol.3
, pp. 545-550
-
-
Williams, R.1
-
24
-
-
31744445498
-
Tiny droplets make a big splash
-
Eisenstein M. Tiny droplets make a big splash. Nat. Methods 3 (2006) 71
-
(2006)
Nat. Methods
, vol.3
, pp. 71
-
-
Eisenstein, M.1
-
25
-
-
0037069324
-
A cell-free protein synthesis system for high-throughput proteomics
-
Sawasaki T., et al. A cell-free protein synthesis system for high-throughput proteomics. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 14652-14657
-
(2002)
Proc. Natl. Acad. Sci. U. S. A.
, vol.99
, pp. 14652-14657
-
-
Sawasaki, T.1
-
26
-
-
16344377955
-
Streamlining Escherichia coli s30 extract preparation for economical cell-free protein synthesis
-
Liu D.V., et al. Streamlining Escherichia coli s30 extract preparation for economical cell-free protein synthesis. Biotechnol. Prog. 21 (2005) 460-465
-
(2005)
Biotechnol. Prog.
, vol.21
, pp. 460-465
-
-
Liu, D.V.1
-
27
-
-
3543116722
-
High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system
-
Endo Y., and Sawasaki T. High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system. J. Struct. Funct. Genomics 5 (2004) 45-57
-
(2004)
J. Struct. Funct. Genomics
, vol.5
, pp. 45-57
-
-
Endo, Y.1
Sawasaki, T.2
-
28
-
-
4644269746
-
High-throughput cell-free systems for synthesis of functionally active proteins
-
Spirin A.S. High-throughput cell-free systems for synthesis of functionally active proteins. Trends Biotechnol. 22 (2004) 538-545
-
(2004)
Trends Biotechnol.
, vol.22
, pp. 538-545
-
-
Spirin, A.S.1
-
29
-
-
0037305599
-
Protein expression systems for structural genomics and proteomics
-
Yokoyama S. Protein expression systems for structural genomics and proteomics. Curr. Opin. Chem. Biol. 7 (2003) 39-43
-
(2003)
Curr. Opin. Chem. Biol.
, vol.7
, pp. 39-43
-
-
Yokoyama, S.1
-
30
-
-
33645286487
-
Enhancing the efficiency of cell-free protein synthesis through the polymerase chain reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues
-
Son J.M., et al. Enhancing the efficiency of cell-free protein synthesis through the polymerase chain reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues. Anal. Biochem. 351 (2006) 187-192
-
(2006)
Anal. Biochem.
, vol.351
, pp. 187-192
-
-
Son, J.M.1
-
31
-
-
33745196264
-
Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system
-
Kim T.W., et al. Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system. J. Biotechnol. 124 (2006) 373-380
-
(2006)
J. Biotechnol.
, vol.124
, pp. 373-380
-
-
Kim, T.W.1
-
32
-
-
1142269592
-
Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis
-
Jewett M.C., and Swartz J.R. Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis. Biotechnol. Prog. 20 (2004) 102-109
-
(2004)
Biotechnol. Prog.
, vol.20
, pp. 102-109
-
-
Jewett, M.C.1
Swartz, J.R.2
-
33
-
-
1542720448
-
Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis
-
Jewett M.C., and Swartz J.R. Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis. Biotechnol. Bioeng. 86 (2004) 19-26
-
(2004)
Biotechnol. Bioeng.
, vol.86
, pp. 19-26
-
-
Jewett, M.C.1
Swartz, J.R.2
-
34
-
-
4644231711
-
Cell-free protein synthesis with prokaryotic combined transcription-translation
-
Swartz J.R., et al. Cell-free protein synthesis with prokaryotic combined transcription-translation. Methods Mol. Biol. 267 (2004) 169-182
-
(2004)
Methods Mol. Biol.
, vol.267
, pp. 169-182
-
-
Swartz, J.R.1
-
35
-
-
27144431943
-
Selecting and screening recombinant antibody libraries
-
Hoogenboom H.R. Selecting and screening recombinant antibody libraries. Nat. Biotechnol. 23 (2005) 1105-1116
-
(2005)
Nat. Biotechnol.
, vol.23
, pp. 1105-1116
-
-
Hoogenboom, H.R.1
-
36
-
-
33645024046
-
Exploiting directed evolution for the discovery of biologicals
-
Douthwaite J., and Jermutus L. Exploiting directed evolution for the discovery of biologicals. Curr. Opin. Drug Discov. Devel 9 (2006) 269-275
-
(2006)
Curr. Opin. Drug Discov. Devel
, vol.9
, pp. 269-275
-
-
Douthwaite, J.1
Jermutus, L.2
-
37
-
-
23644452792
-
Engineered proteins as specific binding reagents
-
Binz H.K., and Pluckthun A. Engineered proteins as specific binding reagents. Curr. Opin. Biotechnol. 16 (2005) 459-469
-
(2005)
Curr. Opin. Biotechnol.
, vol.16
, pp. 459-469
-
-
Binz, H.K.1
Pluckthun, A.2
-
38
-
-
29344448254
-
A new generation of protein display scaffolds for molecular recognition
-
Hosse R.J., et al. A new generation of protein display scaffolds for molecular recognition. Protein Sci. 15 (2006) 14-27
-
(2006)
Protein Sci.
, vol.15
, pp. 14-27
-
-
Hosse, R.J.1
-
39
-
-
18044391361
-
Covalent DNA display as a novel tool for directed evolution of proteins in vitro
-
Bertschinger J., and Neri D. Covalent DNA display as a novel tool for directed evolution of proteins in vitro. Protein Eng. Des. Sel. 17 (2004) 699-707
-
(2004)
Protein Eng. Des. Sel.
, vol.17
, pp. 699-707
-
-
Bertschinger, J.1
Neri, D.2
-
40
-
-
18744362702
-
Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry
-
Sepp A., et al. Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry. FEBS Lett. 532 (2002) 455-458
-
(2002)
FEBS Lett.
, vol.532
, pp. 455-458
-
-
Sepp, A.1
-
41
-
-
0032772534
-
STABLE: protein-DNA fusion system for screening of combinatorial protein libraries in vitro
-
Doi N., and Yanagawa H. STABLE: protein-DNA fusion system for screening of combinatorial protein libraries in vitro. FEBS Lett. 457 (1999) 227-230
-
(1999)
FEBS Lett.
, vol.457
, pp. 227-230
-
-
Doi, N.1
Yanagawa, H.2
-
42
-
-
33747130345
-
Miniaturising the laboratory in emulsion droplets
-
Griffiths A.D., and Tawfik D.S. Miniaturising the laboratory in emulsion droplets. Trends Biotechnol. 24 (2006) 395-402
-
(2006)
Trends Biotechnol.
, vol.24
, pp. 395-402
-
-
Griffiths, A.D.1
Tawfik, D.S.2
-
43
-
-
27744485401
-
Adding amino acids to the genetic repertoire
-
Xie J., and Schultz P.G. Adding amino acids to the genetic repertoire. Curr. Opin. Chem. Biol. 9 (2005) 548-554
-
(2005)
Curr. Opin. Chem. Biol.
, vol.9
, pp. 548-554
-
-
Xie, J.1
Schultz, P.G.2
-
44
-
-
31544483652
-
Biochemistry. Loop grafting and the origins of enzyme species
-
Tawfik D.S. Biochemistry. Loop grafting and the origins of enzyme species. Science 311 (2006) 475-476
-
(2006)
Science
, vol.311
, pp. 475-476
-
-
Tawfik, D.S.1
-
45
-
-
11244281311
-
The 'evolvability' of promiscuous protein functions
-
Aharoni A., et al. The 'evolvability' of promiscuous protein functions. Nat. Genet. 37 (2005) 73-76
-
(2005)
Nat. Genet.
, vol.37
, pp. 73-76
-
-
Aharoni, A.1
-
46
-
-
31544477181
-
Design and evolution of new catalytic activity with an existing protein scaffold
-
Park H.S., et al. Design and evolution of new catalytic activity with an existing protein scaffold. Science 311 (2006) 535-538
-
(2006)
Science
, vol.311
, pp. 535-538
-
-
Park, H.S.1
-
47
-
-
33644986778
-
Frame shuffling: a novel method for in vitro protein evolution
-
Kashiwagi K., et al. Frame shuffling: a novel method for in vitro protein evolution. Protein Eng. Des. Sel. 19 (2006) 135-140
-
(2006)
Protein Eng. Des. Sel.
, vol.19
, pp. 135-140
-
-
Kashiwagi, K.1
-
48
-
-
33744512606
-
Directed evolution of enzymes and biosynthetic pathways
-
Johannes T.W., and Zhao H. Directed evolution of enzymes and biosynthetic pathways. Curr. Opin. Microbiol. 9 (2006) 261-267
-
(2006)
Curr. Opin. Microbiol.
, vol.9
, pp. 261-267
-
-
Johannes, T.W.1
Zhao, H.2
|