Tiny droplets make a big splash

Nature Methods

Volumn 3, Issue 2, 2006, Pages 71-

  Eisenstein, Michael ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ARYLDIALKYLPHOSPHATASE 1; BETA GALACTOSIDASE; ESCHERICHIA COLI PROTEIN; WATER OIL CREAM;

CELL COMPARTMENTALIZATION; CELL FUNCTION; DIRECTED MOLECULAR EVOLUTION; EMULSION; ENZYME ACTIVITY; ENZYME ISOLATION; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC SCREENING; GENETIC TRANSCRIPTION; HIGH THROUGHPUT SCREENING; MICROFLUIDICS; MOLECULAR SIZE; NONHUMAN; NOTE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA TRANSLATION; WHOLE CELL;

4-BUTYROLACTONE; ARYLDIALKYLPHOSPHATASE; BACTERIA; BETA-GALACTOSIDASE; DIRECTED MOLECULAR EVOLUTION; EMULSIONS; ENZYMES; ESCHERICHIA COLI PROTEINS; FLOW CYTOMETRY; GENE EXPRESSION; GENE LIBRARY; MICROFLUIDICS; MINERAL OIL; WATER;

ESCHERICHIA COLI;

EID: 31744445498     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth0206-71     Document Type: Note

References (2)

1. Aharoni, A. et al. High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. Chem. Biol. 12, 1281-1289 (2005).
2. Mastrobattista, E. et al. High-throughput screening of enzyme libraries: in vitro evolution of a β-galactosidase by fluorescence-activated cell sorting. Chem. Biol. 12, 1291-1300 (2005).