Design and evolution of new catalytic activity with an existing protein scaffold

Science

Volumn 311, Issue 5760, 2006, Pages 535-538

  Park, Hee Sung ^a   Nam, Sung Hun ^a   Lee, Jin Kak ^b,e   Yoon, Chang No ^b   Mannervik, Bengt ^c   Benkovic, Stephen J ^d   Kim, Hak Sung ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Institute of Science and Technology   (South Korea)

^c UPPSALA UNIVERSITY   (Sweden)

^d PENNSYLVANIA STATE UNIVERSITY   (United States)

^e Nanormics Inc   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYME KINETICS; ESCHERICHIA COLI; HYDROLYSIS;

GLYOXALASE II; METALLOHYDROLASE SCAFFOLD; PROTEIN ENGINEERING;

PROTEINS;

BETA LACTAMASE; CEFOTAXIME; GLYOXALASE; HYDROLASE;

ENZYME;

ANIMAL CELL; ARTICLE; BACTERIAL GROWTH; CATALYSIS; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; EVOLUTION; GENE DELETION; GENE INSERTION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BETA-LACTAMASES; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CEFOTAXIME; DIRECTED MOLECULAR EVOLUTION; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; HUMANS; HYDROPHOBICITY; IRON; KINETICS; METALS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; THIOLESTER HYDROLASES; ZINC;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 31544477181     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1118953     Document Type: Article

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19. Materials and methods are available as supporting material on Science Online.
20. We thank R. Sterner, M. Meyer, and F. Arnold for helpful comments, and J. H. Kim and S. C. Lee for technical assistance. Supported by National Research Laboratory program and Microbial Genomics and Application Center of Ministry of Science and Technology, Nano-Medical Project of Ministry of Health and Welfare, R & D program of Fusion Strategies for Advanced Technologies of Ministry of Commerce, Industry and Energy, and Brain Korea 21 of Ministry of Education and Human Resources Development Korea.