Enhancing the efficiency of cell-free protein synthesis through the polymerase-chain-reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues

Analytical Biochemistry

Volumn 351, Issue 2, 2006, Pages 187-192

  Son, Jeong Mi ^a   Ahn, Jin Ho ^a   Hwang, Mi Yeon ^a   Park, Chang Gil ^b   Choi, Cha Yong ^a   Kim, Dong Myung ^b  

^a Seoul National University   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

Cell free protein synthesis; Chloramphenicol acetyltransferase; Downstream box; Factor Xa; Translation enhancer

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CELLS; CYTOLOGY; RECOMBINANT PROTEINS;

AMINO ACID RESIDUES; CELL FREE PROTEIN SYNTHESIS; CHLORAMPHENICOL ACETYLTRANSFERASE; DOWNSTREAM BOX; FACTOR XA; PEPTIDE CLEAVAGES; RAPID GENERATIONS; REACTION CONDITIONS;

POLYMERASE CHAIN REACTION;

AMINO ACID; BLOOD CLOTTING FACTOR 10A; CHLORAMPHENICOL ACETYLTRANSFERASE; DETERGENT; OLIGONUCLEOTIDE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL FREE SYSTEM; ENHANCER REGION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

EID: 33645286487     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.11.047     Document Type: Article

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