Conformational changes of the Tet repressor induced by tetracycline trapping

Journal of Molecular Biology

Volumn 279, Issue 2, 1998, Pages 439-447

  Orth, Peter ^a   Cordes, Frank ^a   Schnappinger, Dirk ^b   Hillen, Wolfgang ^b   Saenger, Wolfram ^a   Hinrichs, Winfried ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b Lehrst F Mikrobiol Biochem G   (Germany)

Author keywords

Antibiotic resistance; Inducer binding; Tet repressor; Tetracycline; X ray crystallography

Indexed keywords

DNA; REPRESSOR PROTEIN; TETRACYCLINE;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONFORMATIONAL TRANSITION; GENE EXPRESSION REGULATION; HYDROGEN BOND; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; REPRESSOR GENE; X RAY CRYSTALLOGRAPHY;

EID: 0032486128     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.1775     Document Type: Article

References (26)

1. Berens Ch., Schnappinger D., Hillen W. The role of the variable region in Tet repressor for inducibility by tetracycline. J. Biol. Chem. 272:1997;6936-6942
2. Brent R., Ptashne M. Mechanism of action of the lexA gene product. Proc. Natl Acad. Sci. USA. 78:1981;4204-4208
3. Brünger A.T. The free R-value a novel statistical quantity for assessing the accuracy of crystal structures. Nature. 355:1992;472-475
4. Brünger A.T. X-PLOR Manual, Version 3.8: A System for X-ray Crystallography and NMR. 1996;Yale University Press, New Haven, CT
5. The CCP4 suite programms for protein crystallography. Acta Crystallog. sect D. 50:1994;760-763
6. Engh R.A., Huber R. Accurate Bond and Angle Parameters for X-ray Protein-Structure Refinement. Acta Crystallog. sect A. 47:1991;392-400
7. Ettner N., Müller G., Berens Ch., Backes H., Schnappinger D., Schreppel T., Pfleiderer K., Hillen W. Fast large scale purification of tetracycline repressor variants from overproducing Escherichia coli strains. J. Chromatog. sect. A. 742:1996;95-105
8. Evans S.V. SETOR: Hardware-lighted Three-dimensional Solid Model Representations of Macromolecules. 1993;University of British Columbia, Vancouver, Canada
9. Gilson M.K., Sharp K.A., Honig B.H. Calculating the electrostatic potential of molecules in solution method and error accessment. J. Comput. Chem. 9:1987;327-335
10. Hehre W.J., Burke L.D., Shusterman A.J. A SPARTAN Tutorial Version 3. 0. 1993;Wavefunction, Inc, Irvine, CA
11. Hillen W., Berens Ch. Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu. Rev. Microbiol. 48:1994;345-369
12. Hinrichs W., Kisker C., Duvel M., Müller A., Tovar K., Hillen W., Saenger W. Antibiotic resistance structure of the Tet repressor-tetracycline complex and mechanism of induction. Science. 264:1994;418-420
13. Jogun K.H., Stezowski J.J. Coordination and conformational aspects of oxytetracycline metal ion complexation. J. Am. Chem. Soc. 98:1976;6018-6026
14. Jones T.A. A graphics model building and refinement system for macromolecules. J. Appl. Crystallog. 11:1978;268-272
15. 2+reveals mechanism of antibiotic resistance. J. Mol. Biol. 247:1995;260-280
16. Laskowski R.A., MacArthur M.W., Moss D.S., Thornton J.M. PROCHECK a program to check the stereochemical quality of protein structures. J. Appl. Crystallog. 26:1993;283-291
17. Leslie A.G.W. MOSFLM. Joint CCP4 and ESF-EACMB Newsletter on Protein Crystallography No. 26. 1992;Daresbury Laboratory, Warrington, UK
18. Luzzati V. Traitement statistique des erreurs dans la détermination des structures cristallines. Acta Crystallog. 5:1952;802-810
19. Maniatis T., Fritsch E.F., Sambrook J. Molecular Cloning: A Laboratory Manual. 1982;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
20. Müller G., Hecht B., Helbl V., Hinrichs W., Saenger W., Hillen W. Characterisation of non-inducible Tet repressor mutants suggests conformational changes necessary for induction. Nature Struct. Biol. 2:1995;693-703
21. Nicholls A.J. GRASP Manual. 1993;Columbia University, New York
22. Schnappinger D., Hillen W. Tetracyclines antibiotic action, uptake, and resistance mechanisms. Arch. Microbiol. 165:1996;359-369
23. Schnappinger D., Schubert P., Pfleiderer K., Hillen W. Determinants of protein-protein recognition by four helix bundles changing the dimerization specifity of Tet repressor. EMBO J. 17:1998;535-543
24. Takahashi M., Altschmied L., Hillen W. Kinetic and equilibrium characterization of the Tet-repressor-tetracycline complex by fluorescence measurements; evidence for divalent ion requirements and energy transfer. J. Mol. Biol. 187:1986;341-348
25. van Gunsteren W.F., et al. Biomolecular Simulation: The GROMOS Manual and User Guide. 1996;vdf Hochschulverlag AG, ETH Zürich
26. Yamaguchi A., Udagawa T., Sawai T. Transport of divalent cations with tetracycline as mediated by the transposon Tn 10-encoded tetracycline resistance protein. J. Biol. Chem. 265:1990;4089-4813