The calcium channel α2δ-2 subunit partitions with CaV2.1 into lipid rafts in cerebellum: Implications for localization and function

Journal of Neuroscience

Volumn 26, Issue 34, 2006, Pages 8748-8757

  Davies, Anthony ^a   Douglas, Leon ^a   Hendrich, Jan ^a   Wratten, Jack ^a   Van Minh, Alexandra Tran ^a   Foucault, Isabelle ^a   Koch, Dietlind ^a   Pratt, Wendy S ^a   Saibil, Helen R ^b   Dolphin, Annette C ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

2 ; Calcium; Cerebellum; Channel; Cholesterol; Gabapentin; Lipid raft

Indexed keywords

CALCIUM CHANNEL; CHOLESTEROL; GABAPENTIN; GLYCOSYLATED PROTEIN; LIGAND; MEMBRANE PROTEIN; STOMATIN; VOLTAGE GATED CALCIUM CHANNEL; 4 AMINOBUTYRIC ACID; ALANINE; AMINE; ARGININE; CACNA2D1 PROTEIN, MOUSE; CALCIUM CHANNEL BLOCKING AGENT; CALCIUM CHANNEL N TYPE; CYCLOHEXANECARBOXYLIC ACID DERIVATIVE; NERVE PROTEIN; VOLTAGE DEPENDENT CALCIUM CHANNEL (P Q TYPE); VOLTAGE-DEPENDENT CALCIUM CHANNEL (P-Q TYPE);

ALPHA CHAIN; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CALCIUM CURRENT; CELL MEMBRANE; CEREBELLUM; CONTROLLED STUDY; ELECTROPHYSIOLOGY; IMMUNOBLOTTING; IMMUNOPRECIPITATION; LIPID RAFT; MOLECULAR BIOLOGY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; ANIMAL; CELL LINE; DRUG ANTAGONISM; DRUG EFFECT; ELECTRIC CONDUCTIVITY; GENETICS; METABOLISM; MUTATION; PHYSIOLOGY; PURKINJE CELL; TISSUE DISTRIBUTION;

ALANINE; AMINES; ANIMALS; ARGININE; CALCIUM CHANNEL BLOCKERS; CALCIUM CHANNELS; CALCIUM CHANNELS, N-TYPE; CELL LINE; CEREBELLUM; CHOLESTEROL; CYCLOHEXANECARBOXYLIC ACIDS; ELECTRIC CONDUCTIVITY; GAMMA-AMINOBUTYRIC ACID; IMMUNOPRECIPITATION; MEMBRANE MICRODOMAINS; MICE; MUTATION; NERVE TISSUE PROTEINS; PURKINJE CELLS; TISSUE DISTRIBUTION;

EID: 33748275476     PISSN: 02706474     EISSN: 02706474     Source Type: Journal    
DOI: 10.1523/JNEUROSCI.2764-06.2006     Document Type: Article

References (51)

1. Barclay J, Rees M (2000) Genomic organization of the mouse and human α2δ2 voltage-dependent calcium channel subunit genes. Mamm Genome 11:1142-1144.
2. Barclay J, Balaguero N, Mione M, Ackerman SL, Letts VA, Brodbeck J, Canti C, Meir A, Page KM, Kusumi K, PerezReyes E, Lander ES, Frankel WN, Gardiner RM, Dolphin AC, Rees M (2001) Ducky mouse phenotype of epilepsy and ataxia is associated with mutations in the Cacna2d2 gene and decreased calcium channel current in cerebellar Purkinje cells. J Neurosci 21:6095-6104.
3. 2δ-2 protein with abnormal function. J Biol Chem 277:7684-7693.
4. 3H]leucine. Anal Biochem 255:236-243.
5. 2+ channel gamma subunit genes on chromosome 19q13.43: evolution and expression profile of the gamma subunit gene family. Genomics 71:339-350.
6. o with calcium channels by the calcium channel β-subunit in rat neurones. J Physiol (Lond) 485:365-372.
7. 2δ subunits: structure, functions and target site for drugs. Curr Neuropharmacol 1:209-217.
8. 2+ channels. Proc Natl Acad Sci USA 102:11230-11235.
9. 2+ channels. Annu Rev Cell Dev Biol 16:521-555.
10. Chalfie M, Au M (1989) Genetic control of differentiation of the Caenorhabditis elegans touch receptor neurons. Science 243:1027-1033.
11. Christian AE, Haynes MP, Phillips MC, Rothblat GH (1997) Use of cyclodextrins for manipulating cellular cholesterol content. J Lipid Res 38:2264-2272.
12. 2δ (alpha2delta) subunit mRNA-containing cells in the rat central nervous system and the dorsal root ganglia. J Comp Neurol 491:246-269.
13. De Jongh KS, Warner C, Catterall WA (1990) Subunits of purified calcium channels. α2 and δ are encoded by the same gene. J Biol Chem 265:14738-14741.
14. 2δ calcium channel subunit. Br J Pharmacol 120:833-840.
15. 2 subunits of a DHP-sensitive calcium channel. Science 241:1661-1664.
16. Ertel EA, Campbell KP, Harpold MM, Hofmann F, Mori Y, Perez-Reyes E, Schwartz A, Snutch TP, Tanabe T, Birnbaumer L, Tsien RW, Catterall WA (2000) Nomenclature of voltage-gated calcium channels. Neuron 25:533-535.
17. 2+ entry requires localization in cholesterol-rich domains. J Biol Chem 275:26530-26537.
18. 2+ channel α2δ subunit. J Neurosci 17:6884-6891.
19. Foster LJ, De Hoog CL, Mann M (2003) Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors. Proc Natl Acad Sci USA 100:5813-5818.
20. Glebov OO, Bright NA, Nichols BJ (2006) Flotillin-1 defines a clathrinin-dependent endocytic pathway in mammalian cells. Nat Cell Biol 8:46-54.
21. 2δ subunit subtypes. J Membr Biol 184:35-43.
22. Goodman MB, Ernstrom GG, Chelur DS, O'Hagan R, Yao CA, Chalfie M (2002) MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation. Nature 415:1039-1042.
23. 2δ subunit in current stimulation and subunit interaction. Neuron 16:431-440.
24. 1A- containing high-voltage-activated calcium channels. Biophys J 76:1384-1400.
25. 2δ-2 subunit. Eur J Neurosci 12:1217-1226.
26. 2-subunit and the associated δ peptides. J Biol Chem 266:3287-3293.
27. Klugbauer N, Lacinova L, Marais E, Hobom M, Hofmann F (1999) Molecular diversity of the calcium channel α2-δ subunit. J Neurosci 19:684-691.
28. Klugbauer N, Marais E, Hofmann F (2003) Calcium channel alpha2delta subunits: differential expression, function, and drug binding. J Bioenerg Biomembr 35:639-647.
29. Langhorst MF, Reuter A, Stuermer CA (2005) Scaffolding microdomains and beyond: the function of reggie/flotillin proteins. Cell Mol Life Sci 62:2228-2240.
30. Marais E, Klugbauer N, Hofmann F (2001) Calcium channel alpha(2)delta subunits: structure and gabapentin binding. Mol Pharmacol 59:1243-1248.
31. Mori Y, Friedrich T, Kim M-S, Mikami A, Nakai J, Ruth P, Bosse E, Hofmann F, Flockerzi V, Furuichi T, Mikoshiba K, Imoto K, Tanabe T, Numa S (1991) Primary structure and functional expression from complementary DNA of a brain calcium channel. Nature 350:398-402.
32. Morrow IC, Parton RG (2005) Flotillins and the PHB domain protein family: rafts, worms and anaesthetics. Traffic 6:725-740.
33. V2.2 calcium channel expression. EMBO J 21:1514-1523.
34. Owczarek CM, Treutlein HR, Portbury KJ, Gulluyan LM, Kola I, Hertzog PJ (2001) A novel member of the STOMATIN/EPB72/mec-2 family, stomatin-like 2 (STOML2), is ubiquitously expressed and localizes to HSA chromosome 9p13.1. Cytogenet Cell Genet 92:196-203.
35. Page KM, Heblich F, Davies A, Butcher AJ, Leroy J, Bertaso F, Pratt WS, Dolphin AC (2004) Dominant-negative calcium channel suppression by truncated constructs involves a kinase implicated in the unfolded protein response. J Neurosci 24:5400-5409.
36. Price MP, Thompson RJ, Eshcol JO, Wemmie JA, Benson CJ (2004) Stomatin modulates gating of acid-sensing ion channels. J Biol Chem 279:53886-53891.
37. Rivera-Milla E, Stuermer CA, Malaga-Trillo E (2006) Ancient origin of reggie (flotillin), reggie-like, and other lipid-raft proteins: convergent evolution of the SPFH domain. Cell Mol Life Sci 63:343-357.
38. 1A subunit of brain calcium channels. J Biol Chem 270:21234-21242.
39. 1A subunits of brain calcium channels. J Cell Biol 134:511-528.
40. Scherer PE, Okamoto T, Chun M, Nishimoto I, Lodish HF, Lisanti MP (1996) Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc Natl Acad Sci USA 93:131-135.
41. 2+ current enhancement by α2/δ and β subunits in Xenopus oocytes: contribution of changes in channel gating and α1 protein level. J Physiol (Lond) 489:55-62.
42. Sutton KG, Snutch TP (2001) Gabapentin: a novel analgesic targeting voltage-gated calcium channels. Drug Dev Res 54:167-172.
43. Sutton KG, Martin DJ, Pinnock RD, Lee K, Scott RH (2002) Gabapentin inhibits high-threshold calcium channel currents in cultured rat dorsal root ganglion neurones. Br J Pharmacol 135:257-265.
44. Taverna E, Saba E, Rowe J, Francolini M, Clementi F, Rosa P (2004) Role of lipid microdomains in P/Q-type calcium channel (Cav2.1) clustering and function in presynaptic membranes. J Biol Chem 279:5127-5134.
45. Taylor CP (2004) The biology and pharmacology of calcium channel alpha2-delta proteins Pfizer Satellite Symposium to the 2003 Society for Neuroscience Meeting. Sheraton New Orleans Hotel, New Orleans, LA November 10, 2003. CNS Drug Rev 10:183-188.
46. Toselli M, Biella G, Taglietti V, Cazzaniga E, Parenti M (2005) Caveolin-1 expression and membrane cholesterol content modulate N-type calcium channel activity in NG108-15 cells. Biophys J 89:2443-2457.
47. 2+ influx and synaptic transmission in rat hippocampus and neocortex. Eur J Pharmacol 449:221-228.
48. 2+ channels by gabapentin. Cell Mol Neurobiol 22:185-190.
49. 2+ channels. Trends Neurosci 21:148-154.
50. 2δ for gabapentin binding. Biochem J 342:313-320.
51. Whittaker CA, Hynes RO (2002) Distribution and evolution of von Willebrand/integrin A domains: widely dispersed domains with roles in cell adhesion and elsewhere. Mol Biol Cell 13:3369-3387.